Iindaba
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Yiyiphi indlela yokucoca inkqubo yeprotheni yokucoca
Yiyiphi indlela yokucoca inkqubo yeprotheyini yokucoca? Kuyimfuneko ukwazi ikhowudi ye-DNA yolandelelwano lweprotheyini ecocekileyo, ukubona ukuba zeziphi iiseli okanye izicubu ezichazwe ngokugqithisileyo kwijeni ekujoliswe kuyo, kunye nokuyila iiprimer zegene zokukhulisa i-arf yesiqwenga se-DNA ekujoliswe kuso. Oku kunjalo...Funda ngokugqithisileyo -
Indlela yesigaba esiqinileyo se-microextraction
I-SPME ineendlela ezintathu zokutsalwa ezisisiseko: Direct Ectraction SPME, Headspace SPME kunye ne-membrane-protected SPME. I-1) Ukukhutshwa ngokuthe ngqo kwindlela yokukhupha ngokuthe ngqo, i-quartz fiber ehlanganiswe nesigaba sokumisa i-extraction stationary ifakwe ngokuthe ngqo kwi-matrix yesampuli, kwaye amacandelo ekujoliswe kuwo ...Funda ngokugqithisileyo -
Ukutsalwa kweSigaba esiSiqilima: Ukwahlula siSiseko solu Lungiselelo!
I-SPE ibikho amashumi eminyaka, kwaye ngesizathu esihle. Xa izazinzulu zifuna ukususa amacandelo angasemva kwiisampulu zabo, bajongene nomngeni wokwenza oko ngaphandle kokunciphisa amandla abo okugqiba ngokuchanekileyo nangokuchanekileyo ubukho kunye nobungakanani bekhompawundi zabo ze-interes...Funda ngokugqithisileyo -
Izilumkiso zesixhobo sokutsalwa kwesigaba esiqinileyo
Ukutsalwa kwesigaba esiqinileyo yisampulu yetekhnoloji yonyango ephuhliswe kwiminyaka yakutshanje. Iphuhliswa kwindibaniselwano yotsalo olululwelo oluqinileyo kunye nekholamu yolwelo lwechromatography. Isetyenziselwa ikakhulu ukwahlukana kwesampulu, ukuhlanjululwa kunye nokugxila. Xa kuthelekiswa nolwelo lwemveli-ulwelo ...Funda ngokugqithisileyo -
Indlela yokuchonga ukuba ibhotile yeglasi ifanelekile
Iibhotile zeglasi zihlulwe kulawulo kunye nokubumba ngokweendlela zokuvelisa. Iibhotile zeglasi ezilawulwayo zibhekisa kwiibhotile zeglasi eziveliswe ngeetyhubhu zeglasi. Iibhotile zeglasi ezilawulwayo zibonakaliswa ngumthamo omncinci, iindonga ezilula kunye nezincinci, kwaye kulula ukuthwala. Izinto eziphathekayo zenziwe nge-borosilicate g...Funda ngokugqithisileyo -
Ingxelo yoPhando kwisikali seMarike yeProtein Expression
Ukwenziwa kunye nokulawulwa kweeprotheni kuxhomekeke kwiimfuno zokusebenza kweeseli. Uyilo lweprotheyini lugcinwe kwi-DNA, esetyenziswa njenge template yokuveliswa kwe-RNA yesithunywa ngenkqubo elawulwa kakhulu yokubhala. Iprotein expression yinkqubo apho iiprotheyini zimodif...Funda ngokugqithisileyo -
Ufakelo kunye nokulungiswa kwamanyathelo esixhobo sokutsalwa kwesigaba esiqinileyo
I-Solid phase extraction (SPE) yinkqubo yokukhutshwa komzimba equka izigaba zolwelo kunye neziqinileyo. Kwinkqubo yokukhutshwa, amandla e-adsorption ye-slid ukuya ku-analyte inkulu kune-sample mother yotywala. Xa isampuli idlula kwikholamu ye-SPE, umhlalutyi uyabhengezwa kwi ...Funda ngokugqithisileyo -
Uyicoca njani ibhotile yesampulu yechromatographic
Ibhotile yesampuli isitya sokuhlalutya isixhobo sezinto eziza kuhlalutywa, kwaye ukucoceka kwayo kuchaphazela ngqo umphumo wohlalutyo. Eli nqaku lishwankathela iindlela ezahlukeneyo zokucoca ibhotile yesampulu yechromatographic, kwaye lijonge ukubonelela ngereferensi enentsingiselo kuye wonke umntu. Ezi...Funda ngokugqithisileyo
