I-SPME inezithathu ezisisisekoutsaloiindlela: Direct Ectraction SPME, Headspace SPME kunye ne-membrane-ekhuselwe SPME.

1) Ukutsalwa ngokuthe ngqo

Kwindlela yokukhutshwa ngokuthe ngqo, i-quartz fiber igqunywe ngeutsaloIsigaba esimileyo sifakwa ngokuthe ngqo kwisampulu yematrix, kwaye amacandelo ekujoliswe kuwo akhutshelwa ngokuthe ngqo ukusuka kwisampulu yematriksi ukuya kwisigaba esimileyo sokutsalwa. Ngexesha lemisebenzi yaselabhoratri, iindlela zokuphazamiseka ziqhele ukusetyenziswa ukukhawulezisa ukusasazwa kwamacandelo ohlalutyo ukusuka kwisampulu yematriksi ukuya kumda wesigaba esimileyo sokutsalwa. Kwiisampuli zegesi, i-convection yendalo yegesi yanele ukukhawulezisa ukulingana kwamacandelo okuhlalutya phakathi kwezigaba ezimbini. Kodwa kwiisampulu zamanzi, isantya sokusasazwa kwamacandelo emanzini yi-3-4 yee-odolo zobukhulu obuphantsi kuneegesi, ngoko ke itekhnoloji yokuxuba esebenzayo iyafuneka ukufezekisa ukusasazwa ngokukhawuleza kwamacandelo kwisampulu. Iindlela zokuxuba ezisetyenziswa ngokuqhelekileyo ziquka: ukukhawuleza isantya sokuhamba kwesampuli, ukugubha intloko ye-fiber extraction okanye isitya sesampuli, i-rotor ivuselela kunye ne-ultrasound.

Kwelinye icala, obu buchule bokuxuba bukhawulezisa isantya sokusasazwa kwamacandelo kwisampulu yesampulu enkulu, kwaye kwelinye icala, yehlisa into ebizwa ngokuba “yindawo yelahleko” ebangelwa ngumaleko weshefu yokhuselo yefilim elulwelo eyenziwe phezu. udonga lwangaphandle lwesigaba sokumisa.

2) Ukukhutshwa kwe-Headspace

Kwimowudi yokutsalwa kwesithuba sentloko, inkqubo yokutsalwa inokwahlulwa ibe ngamanyathelo amabini:
1. Icandelo elihlalutyiweyo lisasaza kwaye lingena kwisigaba solwelo ukuya kwisigaba segesi;
2. Icandelo elihlalutyiweyo ligqithiselwa ukusuka kwisigaba segesi ukuya kwisigaba sokumisa.
Olu hlengahlengiso lunokuthintela inqanaba lokucholwa elimileyo ukuba lingangcoliswa zizinto ezinemolekyuli ephezulu kunye nezinto ezingaguquguqukiyo kwiisampulu zematriki ezithile (ezifana neemfihlo zabantu okanye umchamo). Kule nkqubo yokutsalwa, isantya sokutsalwa kwenyathelo lesi-2 sikhulu kakhulu kunesantya sokusasazwa kwenyathelo loku-1, ngoko ke inyathelo loku-1 liba linyathelo lolawulo lokutsalwa. Ke ngoko, amacandelo aguquguqukayo anesantya sokutsalwa esikhawulezayo kunamalungu aguqukayo. Enyanisweni, kumacandelo aguquguqukayo, phantsi kweemeko ezifanayo zokuxuba isampuli, ixesha elilinganayo lokukhutshwa kwe-headspace lifutshane kakhulu kunokukhutshwa ngokuthe ngqo.

I-3) Ukukhutshwa kwe-Membrane

Eyona njongo iphambili yokhuseleko inwebu SPME kukukhuselautsaloinqanaba elimileyo ukusuka kumonakalo xa uhlalutya iisampulu ezimdaka kakhulu. Xa kuthelekiswa ne-SPME ye-headspace extraction, le ndlela inenzuzo ngakumbi ekutsalweni kunye nokutyebisa amacandelo anzima-ukuguquguquka. Ukongezelela, ifilimu ekhuselayo eyenziwe ngezinto ezikhethekileyo inika iqondo elithile lokukhetha inkqubo yokukhutshwa.