Izindaba
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Ithini indlela yokuhlanza yohlelo lokuhlanza amaprotheni
Iyiphi indlela yokuhlanza yohlelo lokuhlanza amaprotheni? Kuyadingeka ukwazi ukulandelana kwekhodi ye-DNA yephrotheni ehlanziwe, ukubona ukuthi yimaphi amaseli noma izicubu ezivezwe ngokweqile kufuzo oluqondiwe, kanye nokuklama ama-primer ofuzo ukuze akhulise i-arf yocezu lwe-DNA oluqondiwe. Lokhu kunjalo...Funda kabanzi -
Indlela ye-microextraction yesigaba esiqinile
I-SPME inezinhlobo ezintathu eziyisisekelo zokukhipha: I-Direct Ectraction SPME, i-Headspace SPME kanye ne-SPME evikelwe ulwelwesi. 1) Isizinda esiqondile Endleleni yokukhipha okuqondile, i-quartz fiber ehlanganiswe nesigaba esimile ifakwa ngokuqondile ku-matrix yesampula, futhi izingxenye eziqondiwe ziyi...Funda kabanzi -
Isizinda Sesigaba Esiqinile: Ukwehlukana Kuyisisekelo salokhu kulungiselelwa!
I-SPE isineminyaka ikhona, futhi ngesizathu esihle. Uma ososayensi befuna ukususa izingxenye ezingemuva kumasampuli abo, babhekene nenselele yokwenza lokho ngaphandle kokunciphisa ikhono labo lokunquma ngokunembayo nangokunembile ubukhona nenani lenhlanganisela yezinto ezithakaselayo...Funda kabanzi -
Izinyathelo zokuphepha zensimbi yesigaba esiqinile
Ukukhishwa kwesigaba esiqinile kuyisampula yobuchwepheshe bokwelashwa obakhiwe eminyakeni yamuva nje. Ithuthukiswa kusukela ekuhlanganisweni kokukhipha okuqinile okuwuketshezi kanye nekholomu ye-chromatography yoketshezi. Isetshenziselwa ikakhulukazi ukuhlukaniswa kwesampula, ukuhlanzwa kanye nokuhlushwa. Uma kuqhathaniswa noketshezi-uketshezi lwendabuko ...Funda kabanzi -
Indlela yokubona ukuthi ibhodlela lengilazi lifanelekile yini
Amabhodlela e-Glass ahlukaniswe ekulawuleni nasekubumbeni ngokwezindlela zokukhiqiza. Amabhodlela engilazi alawulwayo abhekisela emabhodleleni engilazi akhiqizwa amashubhu engilazi. Amabhodlela engilazi alawulwayo abonakala ngomthamo omncane, izindonga ezilula nezincane, futhi kulula ukuthwala. Impahla yenziwe nge-borosilicate g...Funda kabanzi -
Umbiko Wocwaningo Ngesikali Semakethe Se-Protein Expression
Ukwenziwa kanye nokulawulwa kwamaprotheni kuncike ezidingweni zokusebenza kwamaseli. Umklamo wamaprotheni ugcinwa ku-DNA, esetshenziswa njengesifanekiso sokukhiqizwa kwe-RNA yesithunywa ngenqubo elawulwa kakhulu yokubhala. I-protein expression inqubo lapho amaprotheni eguqulwa khona ...Funda kabanzi -
Izinyathelo zokufaka nokulungisa iphutha zedivayisi yokukhipha isigaba esiqinile
I-Solid phase extraction (SPE) inqubo yokukhipha ngokomzimba ehlanganisa izigaba eziwuketshezi neziqinile. Enqubweni yokukhipha, amandla e-adsorption okuqinile kuya ku-analyte makhulu kunesampula yotshwala kamama. Uma isampula idlula kukholomu ye-SPE, i-analyte ikhangiswa ku ...Funda kabanzi -
Indlela yokuhlanza ibhodlela lesampula le-chromatographic
Ibhodlela lesampula liyisitsha sokuhlaziya insimbi yento okufanele ihlaziywe, futhi ukuhlanzeka kwayo kuthinta ngokuqondile umphumela wokuhlaziya. Lesi sihloko sifinyeza izindlela ezahlukahlukene zokuhlanza isampula yebhodlela le-chromatographic, futhi ihlose ukuhlinzeka ngereferensi enengqondo yawo wonke umuntu. Lezi...Funda kabanzi
