Ithini indlela yokuhlanzauhlelo lokuhlanza amaprotheni? Kuyadingeka ukwazi ukulandelana kwekhodi ye-DNA yephrotheni ehlanziwe, ukubona ukuthi yimaphi amaseli noma izicubu ezivezwe ngokweqile kufuzo oluqondiwe, kanye nokuklama ama-primer ofuzo ukuze akhulise i-arf yocezu lwe-DNA oluqondiwe. Lokhu okubizwa ngokuthi ukutholwa kwezingcezu zofuzo eziqondiwe.

Ukwakhiwa kwevektha yenkulumo: Kuvektha ye-prokaryotic noma ye-eukaryotic enezici zofuzo olutholiwe ngokwalo, inkinga enkulu yalesi sinyathelo ukwakha i-plasmid kanye nofuzo lwentshisekelo, kanye nesistimu yokusho. Isikhathi sokukhuluma se-prokaryotic sifushane, izindleko ziphansi, futhi inani elikhulu lokukhuluma libalulekile; isakhi sofuzo asivezwa ku-E. coli, futhi inkinga yenzeka ekusebenziseni i-codon. Ngokucabangela umsebenzi ongcono nokuhlanzwa kwamaprotheni, abacwaningi bakhetha ukuveza imvubelo ye-Pichi. Ukuvezwa okuyimpumelelo kokuthuthukisa i-codon kubalulekile.

Ithini indlela yokuhlanza yohlelo lokuhlanza amaprotheni:

1. Imvula.

2. I-Electrophoresis: Iphrotheni eshajiwe iphakeme noma iphansi kunephoyinti layo le-isoelectric futhi ingathuthelwa ku-electrode eyinegethivu noma ku-electrode ephozithivu yenkambu kagesi. Sekela ifilimu i-electrophoresis, i-electrophoresis, njll.

3. I-Dialysis: Indlela esebenzisa izikhwama ezimbili ze-dialysis ukuhlukanisa ama-molecule amakhulu kumaprotheni kanye nezinhlanganisela ezincane ze-organic molecule.

4. I-Chromatography: I-ion exchange chromatography isebenzisa izakhiwo zamahhala zamaphrotheni. Ngaphansi kwe-pH ethile, izindleko nezakhiwo zamaprotheni zihlukile, futhi zingahlukaniswa nge-ion exchange chromatography. Ku-anion exchange chromatography, amaprotheni anamandla aphansi angemahle akhishwa kuqala. Izisefo zamamolekyuli, ezaziwa nangokuthi ukuhlunga kwejeli. Amaprotheni amancane angena kuma-pores futhi ahlale kuwo isikhathi eside. Amaprotheni amakhulu awakwazi ukungena kuma-pores futhi aphume ngokuqondile.

5. Ithini indlela yokuhlanza yeuhlelo lokuhlanza amaprotheni? I-Ultracentrifugation: Ukuhlanzwa kwamaprotheni kungasetshenziswa ukucacisa isisindo samangqamuzana futhi kungasetshenziswa njengamaprotheni. Ukwakheka kwamaprotheni ane- densities ehlukene kuyahlukaniswa.