ukubuka konke:

Ikholomu epakishwe ngaphambili ye-G-25 iyikholomu yokuhlanza i-desalting ene-dextran njengendawo yokuhlunga ijeli. Izinto ezihlukanisiwe zihlukaniswa ngokwesisindo sengqamuzana ngesisefo semolekyuli yesakhiwo senethiwekhi ye-dextran kukholamu epakishwe kusengaphambili. Ngesikhathi sokuhlukaniswa, ama-molecule amakhulu kunosayizi we-pore wejeli avinjelwa ngaphandle kwesigaba sejeli, futhi afuduke eduze kwegebe phakathi kwezinhlayiya zejeli ngesivinini sokufuduka okusheshayo, futhi akhishwe kuqala. Ama-molecule aphakathi nendawo angena ingxenye yangaphakathi yesigaba sejeli, futhi isivinini se-elution singesesibili; kuyilapho izinto ezincane zamangqamuzana zonke zingena kujeli futhi zithola ukumelana okukhulu, ngakho-ke isiphetho siyasuswa.e

Ikholomu epakishwe kusengaphambili ye-Biomai Life Sciences G-25 inikeza izicaciso ezinhlanu zemikhiqizo: 1, 3, 5, 6, kanye no-12ml, lapho u-1ml no-5ml kusesimweni samakholomu apakishwe ngaphambili we-medium-pressure chromatography, angasebenzisa ngokugcwele okuphakathi. -isistimu yokuhlanza isigaba sokucindezela uketshezi. Izinzuzo, ukususa usawoti ngokushesha kanye nokuhlanzwa kwama-biomacromolecules.

Izici:

★Ukucaciswa okuhlukahlukene: 1/3/6/12mL isesimweni sesirinji, 1/5ml isesimweni sekholomu ye-medium-pressure chromatography;
★Ukumelana nokucindezela okuphezulu: ikholomu yengcindezi ephakathi nendawo epakishwe kusengaphambili ingakwazi ukumelana nokucindezela okufika ku-0.6 MPa (6 ibha, 87 psi);
★Kulula ukusebenzisa: I-Luer interface, ingasetshenziswa ochungechungeni ukwandisa ukulayishwa kwesampula, ingabuye ixhunywe kumasirinji namaphampu e-peristaltic, futhi ingaxhunyaniswa ngokuqondile nezinhlelo zokuhlanza isigaba se-liquid ezifana ne-ÄKTA, i-Agilent, i-Shimadzu, i-Waters, njll. .;
★Uhla olubanzi lwezinhlelo zokusebenza: ukuhlanzwa kwama-nucleic acid, amasosha omzimba, amaprotheni anelebula, ukususa amaprotheni;