Amakholomu&Plates alayishwe ngaphambilini we-Ni-NTA

①Imingcele yomkhiqizo enemininingwane

Isigaba somkhiqizo: Ikholomu/ipuleti le-Ni-NTA lesampula yokulungiselela isampula ye-antibody kanye nokuhlanzwa kwamaprotheni

Material:PP+Agarose ijeli

Umthamo wekholomu: 1ml, 3ml, 5ml, 6ml, 12ml no-2ml 96-hole desalination kanye namapuleti okuhlanza

Umsebenzi: ukukhishwa kwesigaba esiqinile se-protein polypeptide antibody, ukuhlunga, i-adsorption, ukuhlukaniswa, ukukhishwa, ukuhlanzwa kanye nokugxila kwamasampuli okuhlosiwe.

Imininingwane: 0.5ml/1ml, 1ml/3ml, 1.5ml/3ml, 1.5ml/5ml, 2.5ml/5ml, 2ml/6ml, 3ml/6ml, 4ml/12ml, 6ml/14ml,×96, 0.8ml×96

Ukupakishwa: izingcezu ezingama-25 / 1ml, izingcezu ezingama-20 / 3ml, izingcezu ezingama-30 / 6ml, izingcezu ezingama-20 / 12ml, izingcezu ezi-5 / ibhokisi lekholomu ye-chromatography ephakathi, ucezu olu-1 / iphakethe le-96-hole desalination kanye nepuleti yokuhlanza

Izinto zokupakisha: isikhwama se-aluminium foil noma isikhwama se-opaque se-aluminium foil (uma uthanda)

Ibhokisi lokupakisha: ibhokisi lelebula elingathathi hlangothi noma ibhokisi lombala le-BML (kuyakhetheka)

I-LOGO ngokwezifiso: yebo

imodi yokuhlinzeka: OEM/ODM

②Incazelo yomkhiqizo

Ikholomu ye-BM yesayensi yezempilo ye-Ni-NTA yahlanzwa nge-protein affinity chromatography ne-agarose njengendlela exhumanisayo. , inani elikhulu lokuhlanzwa, ukuhlanzwa okulula kwamaprotheni adingekayo.

I-Nickel chloride kukholomu ye-Ni ingaboshelwa ephrotheni ngelebula yakhe (ye-histone). Ngemva kokubopha, iphrotheni eqondiwe yemvelo ingatholwa nge-imidazole elution.Imingcele yokupakisha yekholomu ye-Ni kusayensi yempilo yamamayela ayikhulu.

I-Substrate: 4% noma 6% i-agarose exhumene;

Isilinganiso sosayizi wezinhlayiyana: 90um(45-165um);Isixazululo sesitoreji: isixazululo se-ethanol esingu-20%;

Ukuzinza kwe-PH: 2-12;

Ama-denaturants amelana: 6M i-guanidine hydrochloride ne-8M urea;

Ukumelana nokucindezela: <0.3mpa, 0.1mpa kunconywa;

I-Adsorption: > 20mg(27kDa) amaprotheni / ml;

Izimo zokugcina: 2-8℃.

Ikholomu ye-BM elayishwe kusengaphambili ihlinzeka ngemikhiqizo yezicaciso ezingu-5, okuhlanganisa u-1, 3, 5, 6 kanye no-12ml. I-1ml kanye no-5ml amakholomu alayishwe kusengaphambili we-chromatography yokucindezela okuphakathi, engasebenzisa ngokugcwele izinzuzo zesistimu yokuhlanza uketshezi lwe-medium pressure ukuze kukhishwe usawoti emanzini ngokushesha nangempumelelo kanye nokuhlanzwa kwama-macromolecule ebhayoloji.

③Izici zomkhiqizo:

★imininingwane ehlukahlukene: 1/3/6/12ml yesirinji, 1/5ml yekholomu ye-chromatography ephakathi;

★ingcindezi ephezulu yokubekezelelana: i-medium pressure chromatography efakwe ngaphambili ingcindezi yokubekezelela ikholomu kuze kufike ku-0.6mpa (6 bar, 87 psi);

★kulula ukuyisebenzisa: i-ruer interface, ingasetshenziswa ochungechungeni ukwandisa ukulayisha isampula, ingabuye ixhunywe ku-syringe kanye nepompo ye-peristaltic, ingabuye ixhunywe ngokuqondile i-KTA, i-Agilent, i-Shimadzu, i-Waters nezinye izinhlelo zokuhlanza isigaba samanzi;

★esetshenziswa kabanzi: i-nucleic acid ehlanjululwe, amasosha omzimba, amaprotheni anelebula, ukuchithwa kwamaprotheni.

Order Ulwazi

Incazelo yombala wenombolo yekati (ml) ukupakisha

PNI001-1 obomvu/oluhlaza okotshani NI medium pressure chromatography ikholomu efakwe ngaphambili 1 5 / ibhokisi

I-PNI001-2 ithubhu esobala/ikhava ebomvu uhlobo lwesirinji ye-NI elayishwe ngaphambili ikholomu 1 25 / ibhokisi

PNI003-1 ishubhu esobala/ikhava ebomvu NI uhlobo lwesirinji elayishwe ngaphambilini ikholomu 3 20 / ibhokisi

PNI005-1 obomvu/oluhlaza okotshani NI medium chromatography ikholomu efakwe ngaphambili 5 5 / ibhokisi

I-PNI006-1 ishubhu esobala/ikepisi elibomvu uhlobo lwesirinji ye-NI elayishwe ngaphambili ikholomu 6 30 / ibhokisi

I-PNI012-1 ithubhu esobala/ikhava ebomvu uhlobo lwesirinji ye-NI elayishwe ngaphambili ikholomu 12 20 / ibhokisi

I-BM Life Science, lonke uchungechunge lwamashubhu ekholomu ye-SPE enziwe ngomjovo we-polypropylene wezinga lezokwelapha;Ipuleti lesisefo lifakwe i-ultra-pure ultra-high molecular weight polyethylene.sorbents athengwe emhlabeni wonke, futhi ngokuhlolwa okugunyaziwe, ikhwalithi ithembekile;10,000 ukukhiqizwa kweshabhu lokusebenzela elihlanzekile, inqubo yokukhiqiza esezingeni, ukuphathwa kwe-ERP, ukulandeleka kwekhwalithi yomkhiqizo; ukucaciswa kwemikhiqizo ehlukahlukene ukuze kuhlangatshezwane nezidingo zamakhasimende; yonke imikhiqizo yethu yenziwe ngendlela oyifisayo ukuze amakhasimende akwazi ukujabulela isevisi yeqophelo eliphezulu.

Izici zemikhiqizo yochungechunge lwe-BM Life Science SPE

Izinzuzo zobuchwepheshe:

★Olunye ucwaningo lwe-SPE sorbents kanye nokuthuthukiswa nobuchwepheshe bokukhiqiza obunamalungelo azimele empahla yengqondo, ukulayishwa kwe-SPE (ukusabalalisa impuphu, ukulayisha, ukupakishwa) ku-othomathikhi ngokugcwele

★Ngokuthembela ezinzuzweni eziyingqayizivele zemboni yokubumba egxilile yesikhunta se-CNC endaweni yomfula i-pearl river delta, ukuhlanganiswa kanye nokusetshenziswa kahle kwezinsiza kuphindaphinde kabili umthamo wokukhiqiza wamapayipi ekholomu ye-SPE, kwehliswe ngesigamu izindleko zokukhiqiza isikhunta nokubumba umjovo, futhi kwathuthukisa kakhulu izinga lomkhiqizo

★Inkampani inobuchwepheshe obukhethekile bokusabalalisa impushana eyingqayizivele, ukusatshalaliswa kwempushana ngokuphelele ngendlela ezenzakalelayo, eqoqwayo, ehlanjululwayo yokuqinisekisa ukuthi iqoqo lomkhiqizo lizinze kakhudlwana.

★Amakhulu ezigidi ze-R&D namandla okukhiqiza amapuleti esisefo kanye nomgogodla wokuhlunga kwehlise izindleko zokukhiqiza ze-SPE zaya ezingeni eliphansi kakhulu.

★Ipuleti lesisefo le-SPE lizimele ngokuphelele, ububanzi balo, ukujiya, usayizi wokuvula kungakhethwa futhi kuhlanganiswe ngokuthanda.

★inkampani ibheka ukubaluleka okukhulu ekusungulweni kwezobuchwepheshe kanye nokwenza ngcono okuqhubekayo, ikakhulukazi i-Tip SPE, izisefo zephaneli yamahhala yokufaka i-SPE, ipuleti le-96-384-hole SPE elinamalungelo obunikazi obuhlakani obuzimele, elivala igebe eChina futhi lifinyelele ezingeni lomhlaba wonke, libonisa izinzuzo eziyingqayizivele ze-B&M Life Science emkhakheni we-SPE.

Izinzuzo zomkhiqizo:

★Kulula ukusebenza, ngaphansi kwesenzo samandla adonsela phansi emvelo kungafinyelela ibanga elihle kakhulu lesivinini kanye nokuphindaphinda okuhle

★Ingonga izindleko zemishini nezinto ezisetshenziswayo kakhulu ngaphandle kwe-SPE kanye nemishini yokuhlanza

★i-sorbent ehlanzekile ngaphandle kokuphazamiseka okuvela kungemuva elingenalutho

★izinga eliphezulu lokugaywa kabusha kwe-10~100ppm belisebangeni elingcono kakhulu lika-95%~105%

★Ngomthamo omkhulu we-adsorption, iphakeme kunezinye izinhlobo zekholomu ye-SPE e-China

★ikhwalithi yomkhiqizo ezinzile, ukukhiqiza kabusha okuhle, layisha ukuchezuka okujwayelekile okuhlobene (RSD) <5%

★Ayesabi ikholomu eyomile.Ukugeleza okomile nokumanzi kuyafana phakathi kwebanga lephutha, ukuchezuka okujwayelekile okuhlobene (RSD) <0.05%

★imikhiqizo yethu isezingeni elifanayo lekhwalithi njenge-Waters / Agilent/ Supelco nezinye izinkampani

★Izinga lokusebenza kwezindleko zemikhiqizo yethu liphakathi kwezingcono kakhulu emhlabeni

Isicelo:

★Kusetshenziswa kakhulu enhlabathini;Amafutha;uketshezi lomzimba (i-plasma/umchamo, njll.);Ukudla nokunye

Ukuzibophezela kwekhwalithi:

★Qinisekisa ukuthi wonke umkhiqizo ufanelekile, sebenzisa izindinganiso eziqinile zokulawula ikhwalithi, sebenzisa ukuhlolwa kwenqwaba

★Qinisekisa ukuthi umkhiqizo ngamunye awunakho ukuphazamiseka okungenalutho, izinga lokubuyisela lingcono kunemithetho kazwelonke, ukuze ufinyelele izinga eliphezulu lemikhiqizo efanayo.

Izithembiso zokusebenza:

★Nikeza ngosekelo lwezobuchwepheshe oluchwepheshile mahhala

Ukuhlelwa kwekholomu ye-SPE

Uchungechunge lwe-polymer matrix SPE

Nge-polymer eyindilinga njenge-adsorbent, usayizi wezinhlayiyana ze-sorbent ufana kakhulu, izinga lokugeleza kwekholomu ye-SPE lizinze kakhulu, okuyikholomu ye-polymer yakudala elabhorethri, lonke uchungechunge lwemikhiqizo lusetshenziswe kabanzi ekuhlolweni kokudla. Imikhiqizo ilingana ne-Waters' HLBS, MAX's kanye ne-MCX's.

I-silica gel matrix uchungechunge SPE

Ikholomu ye-SPE ye-silica gel matrix yakudala, i-amorphous/spherical adsorbent, esebenza ngezindleko eziphakeme kanye nolwazi oluhle lomsebenzisi olunikezwa ikhasimende elithembayo.Imikhiqizo isetshenziswa kakhulu ekuhlaziyeni amakhemikhali.

Ukukhishwa kwethiphu/ukuhlanzwa/ukugxiliswa kwe-SPE

iyithuluzi lokubhobhoza lomkhonto elisebenza ngokusetshenziswa okusetshenziselwa ukukhishwa/ukuhlanzwa okunothisa amasampula ebhayoloji noma i-Tip of SPE, inhloso yomkhiqizo we-pipetting top spear top engeza i-C4 / C18 / i-silicon powder/magnetic beads/ProtingA (G) i-agarose gel sorbent, esetshenziselwa primer/genomic DNA plasmid PCR imikhiqizo/peptide protein/antibodies to target product/filter/extraction/desalting/purification/enothisa.

96/384-imbobo plate plate uchungechunge SPE

Uchungechunge lwepuleti le-96/384-well SPE lwenzelwe ngokukhethekile ukwelapha isampula ephezulu.Qedela inqubo yokucubungula ngaphambilini yesampula ngesihlungi se-orifice senkampani noma indawo yokusebenza ezenzakalelayo.

Uchungechunge lokutholwa okukhethekile SPE

Ikholomu yokutholwa kwedayi ekhethekile ye-Azo: khetha isigcwalisi se-diatomite esihlanzekile kakhulu;Ubuchwepheshe bokulawula izinga lokugeleza kwepuleti elikhethekile, ngokuhlolwa kwezikhungo ezigunyaziwe zasekhaya nezangaphandle, ukunikezwa kwe-OEM.

Ngaphezu kwalokho, kukhona ikhabhoni emnyama eyi-graphitized, i-alumina engathathi hlangothi ene-asidi ye-alkali, igobolondo likakhukhunathi ikhabhoni ecushiwe, ikholomu yokutholwa koju, ikholomu yokususwa kombala kwesikhumba, ikholomu yokutholwa kwe-plasticizer……ikholomu ye-SPE, eminye imikhiqizo ye-SPE sicela ubuze.

Izinyathelo (thatha Ithiphu SPE njengesibonelo) :

Umsebenzi wehluke kancane ngokuya ngendlela yokugcinwa kwe-sorbent ene-adsorption yemikhiqizo eqondiwe noma ukungcola.

1. I-sorbent ikhangisa umkhiqizo okuhlosiwe, isuse ukungcola futhi ibaleke umkhiqizo okuhlosiwe

Isimiso:

ukulayisha ukuhlanzwa kwesampula, ukuwasha okwenqatshwa, i-lution

Lolu hlobo lomsebenzi wokukhipha isigaba esiqinile ngokuvamile lunezinyathelo ezine:

(1) ukwenza kusebenze - ukususwa kokungcola ku-Tip SPE kanye nokukhiqizwa kwendawo ethile encibilikayo;

(2) isampula yomthwalo - ncibilikisa isampula ku-solvent ethile, sebenzisa i-pipette ukuhogela i-Tip SPE futhi ugcine izingxenye kuThiphu;

(3) ukukhishwa kwe-leaching - ukukhishwa okuphezulu kokungcola neminye imikhiqizo okungahlosiwe;

(4) i-elution - ukukhishwa kanye nokuqoqwa komkhiqizo okuhlosiwe ngevolumu encane ye-solvent.

2. I-sorbent ikhangisa ukungcola futhi isuse umkhiqizo ohlosiwe

Isimiso:

Adsorb ukungcola kusampula futhi ukhiphe umkhiqizo oqondiwe

Lolu hlobo lokukhishwa kwesigaba esiqinile ngokuvamile lunezinyathelo ezintathu:

(1). Ukwenza kusebenze — susa ukungcola ku-Tip SPE bese ukhiqiza indawo ethile encibilikayo.

(2) isampula yokulayisha — phefumula i-Tip SPE nge-pipette bese uyiqhumisa kancane. Ngalesi sikhathi, izinhlanganisela eziningi eziqondiwe zizoshaywa umoya ngesixazululo sesisekelo sesampula, futhi ukungcola kuzogcinwa kokuthi Ithiphu. Ngakho-ke, izinyathelo kufanele ziqalwe ukuqoqa.

(3) I-Elution - ukukhipha izakhi ngomthamo omncane we-solvent kanye nokuqoqwa, kuhlanganisa uketshezi oluqoqwe.

Ukucaciswa okwengeziwe noma ukwenza ngezifiso, wamukelekilewonke amakhasimende amasha namadala ukubuza, ukuxoxa ngokubambisana, ukufuna intuthuko efanayo!