Ochratoxin Affinity Chromatography

Umgaqo wokucoca wokufunyanwa kwe-ochratoxin ikholamu ekhethekileyo yimpendulo yomzimba yokuzikhusela phakathi kwe-antigen antibody. I-antibody ye-monoclonal equlethe i-ochratoxin igxininiswe kwinkxaso yesigaba esiluqilima sekholamu yokubhaqa, isicatshulwa sesampulu esiqulethe i-ochratoxin yasetyenziselwa ukukhangela ikholamu ekhethekileyo nge-ochratoxin, Ibophelela kwi-antibody, yenze i-antigen antibody complex, emva koko iyihlambe ngamanzi. ukuya kwizinto ekujoliswe kuzo. Okokugqibela, eluting kunye ne-eluent, qokelela i-eluting fluid kwaye usebenzise i-HPLC ukubona imixholo ye-ochratoxin.


Iinkcukacha zeMveliso

Iithegi zeMveliso

I-Ochratoxin yi-metabolite yesibini eyenziwe yi-fungi ye-aspergillus kunye ne-penicillium, eyona nto i-toxin enamandla yezintso kunye ne-toxin yesibindi kwaye isetyenziswa ngokubanzi kwizidlo ezahlukeneyo. Uvavanyo lwezilwanyana lubonisa ukuba ityhefu ebukhali okanye engapheliyo inokwenzeka emva kokutya ukutya okungcoliswe yityhefu. Ukutya kunye nokutya okuthintela ukosuleleka kwe-ochratoxin A ngokuthe ngqo okanye ngokungathanga ngqo kwikhonkco lokutya lomntu, Kubaluleke kakhulu ukomeleza ukubonwa kwe-ochratoxin A.

I-B&M ye-ochratoxin yokufumanisa uluhlu olukhethekileyo lwekholamu ikakhulu luvavanyo olukhethekileyo lwe-ochratoxin yovavanyo lobudlelwane bomzimba. Ikholomu inokukhetha ngokukhetha i-ochratoxin kwisisombululo sesampuli, ngoko ke umphumo wokuhlanjululwa kwekholomu unokujoliswa, kwaye isampuli inokuvavanywa ngokuthe ngqo yi-HPLC emva kokuba ikholamu ihlanjululwe.

Isicelo:
Uziinkozo; isondlo; ukutya; iziselo, njl.
Usetyenziso oluqhelekileyo:
Isetyenziselwa ukucocwa kwe-ochratoxin kwisampulu
yobunzima obuphantsi bematrix kunye neemfuno ezinyiniweyo. Iyasetyenziswa
yokucoca i-ochratoxin kwisampulu ephantsi
i-matrix complex kunye neemfuno ezilinganiselweyo.Ubungakanani
uhlalutyo lwe-TLC/HPLC/GC/lc-ms/EIA;
Inokuvavanywa ngokobungakanani be-ochratoxin kwiinkozo,
ukutya, umgubo, ibhiya, iwayini kunye nesiselo.

Ulwazi lweodolo

Ii-sorbents

Ifom

Inkcazo

IiPC/pk

Ikati.No

ICartridge yokufumanisa i-OTA Ikhatriji 1mL

25

OTA-IAC0001
ICartridge yokufumanisa i-OTA   3mL

20

OTA-IAC0003
Ikholamu engenanto ye-affinity chromatography   1mL, amaqhekeza amabini e-hydrophilic Frits

100

ACC001
Ikholamu engenanto ye-affinity chromatography   3mL, amaqhekeza amabini e-hydrophilic Frits

50

ACC003

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