Fumonisin Affinity Chromatography

Umgaqo wokucoca wekholamu ekhethekileyo yokubona i-Fumonisin yimpendulo yokuzivikela phakathi kwe-antigens. Ii-antibodies ze-Monoclonal eziqulethe uvavanyo lwe-Fumonisins zilungiswe kwinkxaso yesigaba esiqinileyo sekholamu yokufumanisa. Isampulu yesampula equlethe i-Fumonisin isetyenziselwa ukukhangela ikholamu ekhethekileyo ye-Fumonisins, enokuthi idityaniswe ne-antibody ukuze yenze i-antigen antibody complex, ehlanjwe ngamanzi ukuya kwizinto ezijoliswe kuzo. Okokugqibela, eluting kunye eluent, qokelela eluent, sebenzisa HPLC ukubona umxholo Fumonisin.


Iinkcukacha zeMveliso

Iithegi zeMveliso

I-Fumonisins yityhefu yomngundo eveliswa ziscythe, ngoku kukho izinto eziphuma kwi-28 ezaziwayo, Esinye sezifundo eziqhelekileyo kunye nezinzulu yi-FB1. Kufunyaniswe ukuba umbona kunye neemveliso zawo, ezifana nokutya kwezilwanyana, zingcoliseka lula yi-FB1. I-FB1 yeyona inamandla kwiiFumonisins kwaye inefuthe eliyingozi kwizilwanyana ezininzi. Uphononongo lubonise ukuba ihashe elimhlophe le-FB1 linokubangela ukuthamba kwesifo sobuchopho, i-swine pulmonary edema syndrome, ukongeza, isenokubangela umhlaza wommizo kunye nomhlaza wesibindi, umhlaza wesisu kunye nezinye izifo, ukufuywa kwezilwanyana kunye nempilo yabantu. ikwabalulekile.

Uluhlu oluphambili lwe-B & M yokufumanisa i-toxin ikholomu ekhethekileyo yokufumanisa i-immuno-affinity kunye nekholamu ekhethekileyo ye-FB1 (FB1) .Le kholamu inokukhetha ngokukhetha i-vomatoxin B1 (FB1) kwisisombululo sesampuli, ngoko ke umphumo wokuhlanjululwa kwekholomu unokujoliswa. , kwaye isampuli inokuvavanywa ngokuthe ngqo yi-HPLC emva kokuba ikholamu ihlanjululwe.

Isicelo:
Umbona; isondlo; ioli etyiwayo, njl.
Usetyenziso oluqhelekileyo:
Isetyenziselwa ukuhlanjululwa kweFumonisin
i-substrates entsonkothileyo kunye nomda ophantsi. Uhlalutyo lobungakanani
ye-TLC/HPLC/GC/LC-MS/EIA;
Inokulinganisa ngokomgangatho nangobungakanani
isixa esishiyekileyo seFumonisin kumbona, ukutya, ioli etyiwayo
kunye nezinye iisampuli

Ulwazi lweodolo

Ii-sorbents

Ifom

Inkcazo

IiPC/pk

Ikati.No

Ikhatriji yokubona iFumonisin Ikhatriji 1mL

25

FB-IAC0001
Ikhatriji yokubona iFumonisin   3mL

20

FB-IAC0003
Ikholamu engenanto ye-affinity chromatography   1mL, amaqhekeza amabini e-hydrophilic Frits

100

ACC001
Ikholamu engenanto ye-affinity chromatography   3mL, amaqhekeza amabini e-hydrophilic Frits

50

ACC003

  • Ngaphambili:
  • Okulandelayo:

  • Bhala umyalezo wakho apha kwaye uwuthumele kuthi