kakaretso:

Kholomo e pakiloeng esale pele ea G-25 ke kholomo ea tlhoekiso ea desalting e nang le dextran joalo ka mokhoa oa ho tlhotla gel. Lintho tse arohaneng li arotsoe ho latela boima ba molek'hule ka sieve ea molek'hule ea sebopeho sa marang-rang sa dextran kholomong e pakiloeng pele. Nakong ea karohano, limolek'hule tse kholo ho feta boholo ba pore ea gel li koetsoe ka ntle ho karolo ea gel, 'me li falla hammoho le lekhalo pakeng tsa likaroloana tsa gel ka lebelo la ho falla ka potlako,' me li hlahisoa pele. Limolek'hule tsa boholo bo bohareng li kena ka hare ho karolo ea gel, 'me lebelo la elution ke la bobeli; ha lintho tse nyenyane tsa molek'hule kaofela li kena ka gel 'me li fumana khanyetso e kholo, kahoo qetello e e-luted.e

Kholomo e kentsoeng pele ea Biomai Life Sciences G-25 e fana ka lintlha tse hlano tsa lihlahisoa: 1, 3, 5, 6, le 12ml, tseo 1ml le 5ml li leng ka sebopeho sa likholomo tse kentsoeng pele tsa chromatography, tse ka sebelisang bohareng. -sistimi ea tlhoekiso ea mokelikeli oa khatello. Melemo, desalting ka potlako le tlhoekiso ea biomacromolecules.

Likaroloana:

★Litlhaloso tse fapaneng: 1/3/6/12mL e ka sebopeho sa seringe, 1/5ml e ka sebopeho sa kholomo ea chromatography ea khatello e mahareng;
★Ho hanyetsa khatello e phahameng: chromatography e nang le khatello e bohareng e ka mamella khatello e phahameng ho 0.6 MPa (6 bar, 87 psi);
★E bonolo ho e sebelisa: Luer interface, e ka sebelisoa letotong ho eketsa sampuli ea loading, e ka boela ea kopanngoa le li-syringe le lipompo tsa peristaltic, hape e ka kopanngoa ka ho toba le mekhoa ea ho hloekisa karolo ea metsi e kang ÄKTA, Agilent, Shimadzu, Waters, joalo-joalo. .;
★Mekhahlelo e mengata ea ts'ebeliso: tlhoekiso ea li-nucleic acid, li-antibodies, liprotheine tse ngotsoeng, ho tlosa letsoai la protheine;