Likholomo le Lipoleiti tse Kentsoeng ke Ni-NTA

①Lintlha tse qaqileng tsa lihlahisoa tsa lihlahisoa

Sehlopha sa lihlahisoa: Kholomo/plate ea Ni-NTA bakeng sa ho lokisoa ha sampole ea li-antibody tsa protein peptide le tlhoekiso ea protheine

lintho tse bonahalang: PP + Agarose gel

Bolumo ea kholomo: 1ml, 3ml, 5ml, 6ml, 12ml le 2ml 96-hole desalination le lipoleiti tsa tlhoekiso

Mosebetsi: ho ntšoa ha karolo e tiileng ea protheine ea polypeptide antibody, filtration, adsorption, karohano, ho ntšoa, tlhoekiso le khatello ea lisampole tse shebiloeng.

Litlhaloso: 0.5ml/1ml, 1ml/3ml, 1.5ml/3ml, 1.5ml/5ml, 2.5ml/5ml, 2ml/6ml, 3ml/6ml, 4ml/12ml, 6ml/12ml,×96, 0.8ml×96

Ho paka: likotoana tse 25 / 1ml, likotoana tse 20 / 3ml, likotoana tse 30 / 6ml, likotoana tse 20 / 12ml, likotoana tse 5 / lebokose la kholomo ea chromatography e mahareng, sengoathoana se le seng / pakete ea desalination ea masoba a 96 le poleiti ea tlhoekiso.

Lintho tsa ho paka: mokotla oa foil oa aluminium kapa mokotla oa aluminium opaque (ka boikhethelo)

Lebokose la liphutheloana: lebokose la laibole le sa nke lehlakore kapa lebokose la mebala la BML (ho ikhethela)

Tloaelo ea Logo: ho joalo

mokhoa oa phepelo: OEM/ODM

②Tlhaloso ea Sehlahisoa

BM life science Ni-NTA column was purified by protein affinity chromatography with agarose as crosslinking medium.Molao-motheo oa tlhoekiso ke ho hloekisa protheine e lebisitsoeng ka tag ka ho sebelisa thepa e itseng e tlamang pakeng tsa li-ion tsa nickel chelated ho NTA le tag.Mokhoa ona o bonolo ho o sebetsa. , palo e kholo ea tlhoekiso, ho bonolo ho hloekisa liprotheine tse hlokahalang.

Nickel chloride e ho Nillum ea Ni e ka tlamahanngoa le protheine ka leibole ea hae ea (histone). Ka mor'a ho tlama, protheine ea sepheo sa tlhaho e ka fumanoa ka imidazole elution.Packing parameters of Ni column in hundred miles life science.

Substrate: 4% kapa 6% cross-linked agarose;

Karolelano ea boholo ba likaroloana: 90um (45-165um); Tharollo ea polokelo: 20% tharollo ea ethanol;

PH botsitso: 2-12;

Li-denaturants tse hanyetsanang: 6M guanidine hydrochloride le 8M urea;

Ho mamella khatello: <0.3mpa, 0.1mpa e khothalletsoa;

Adsorption:> 20mg (27kDa) protheine / ml;

Maemo a polokelo: 2-8℃.

BM life science Ni kholomo e jarollotsoeng e fana ka lihlahisoa tsa lintlha tse 5, ho kenyeletsoa 1, 3, 5, 6 le 12ml. 1ml le 5ml ke litšiea tse kentsoeng esale pele tsa chromatography ea khatello e mahareng, e ka sebelisang ka botlalo melemo ea sistimi ea tlhoekiso ea mokelikeli o mahareng bakeng sa ho tlosa letsoai le letsoai le tlhoekiso ea li-macromolecules tsa tlhaho.

③Likarolo tsa sehlahisoa:

★lintlha tse fapaneng: 1/3/6/12ml bakeng sa sering, 1/5ml bakeng sa kholomo e mahareng ea chromatography;

★khatello e phahameng ea mamello: khatello e bohareng ea chromatography e kentsoeng pele ho mamello ea kholomo ho fihlela ho 0.6mpa (6 bar, 87 psi);

★e bonolo ho e sebelisa: ruer interface, e ka sebelisoa letotong ho eketsa sampuli ea ho jara, e ka boela ea kopanngoa le syringe le pompo ea peristaltic, e ka boela ea kopanngoa ka ho toba KTA, Agilent, Shimadzu, Waters le mekhoa e meng ea ho hloekisa mohato oa metsi;

★e sebelisoang haholo: nucleic acid e hloekisitsoeng, li-antibodies, protheine e ngotsoeng, ho tlosoa ha letsoai la protheine.

Order Information

Cat No. color description specification (ml) packing

PNI001-1 e khubelu / e tala NI e mahareng khatello ea chromatography e kentsoeng pele kholomo ea 1 5 / lebokose

PNI001-2 pepeneneng tube / sekoahelo se khubelu NI mofuta oa seringe preloaded kholomo 1 25 / lebokose

PNI003-1 pepeneneng tube / sekoahelo se khubelu NI mofuta oa syringe pele ho jara kholomo 3 20 / lebokose

PNI005-1 e khubelu / e tala NI medium chromatography e kentsoeng pele kholomo ea 5 5 / lebokose

PNI006-1 pepeneneng tube / sekoahelo se khubelu NI mofuta oa seringe pele ho laeloa kholomo 6 30 / lebokose

PNI012-1 pepeneneng tube / sekoahelo se khubelu NI mofuta oa syringe pele ho jara kholomo 12 20 / lebokose

BM Life Science, letoto lohle la li-tubes tsa SPE li entsoe ka ente ea polypropylene ea boemo ba bongaka; poleiti ea sieve e tšeloa ka polyethylene.sorbents ea boima ba limolek'hule e phahameng haholo e rekiloeng lefatšeng ka bophara, 'me ka tlhahlobo e matla, boleng boa tšepahala;10,000 tlhahiso ea "workshop" e hloekileng, ts'ebetso e tloaelehileng ea tlhahiso, taolo ea ERP, ts'ebetso ea boleng ba sehlahisoa; litlhaloso tse fapaneng tsa lihlahisoa ho fihlela litlhoko tsa bareki; lihlahisoa tsohle tsa rona li etselitsoe ho thusa bareki ho natefeloa ke tšebeletso ea boleng bo holimo.

Likarolo tsa lihlahisoa tsa letoto la BM Life Science SPE

Melemo ea tekheniki:

★Lipatlisiso tse ling tsa li-sorbents tsa SPE le nts'etsopele le theknoloji ea tlhahiso e nang le litokelo tse ikemetseng tsa thepa ea mahlale, ho kenya SPE (kabo ea phofshoana, ho kenya, ho paka) ho iketselitse ka botlalo.

★Ho ipapisitse le melemo e ikhethang ea indasteri ea ho etsa ente ea hlobo ea CNC sebakeng sa noka ea perela, kopanyo le ts'ebeliso e nepahetseng ea lisebelisoa li ekelitse matla a tlhahiso ea liphaephe tsa likholomo tsa SPE habeli, ho fokotsa litšenyehelo tsa ho etsa hlobo le ho bopa ente, mme ho ntlafalitse haholo boleng ba sehlahisoa

★Khamphani e na le theknoloji e ikhethang ea kabo ea phofshoana, kabo ea phofo ka botlalo ka mokhoa o ikemetseng, o ka qhekelloang, o scalable ho netefatsa hore batch ea sehlahisoa e tsitsitse haholoanyane.

★Makholo a limilione a R&D le bokhoni ba tlhahiso bakeng sa lipoleiti tsa sieve le sefa mantlha li fokolitse theko ea tlhahiso ea SPE ho ea boemong bo tlase.

★SPE sieve poleiti e ikemetse ka ho feletseng, bophara ba eona, botenya, boholo ba lesoba le ka khethoa 'me la aroloa ka thato.

★Khampani e nka bohlokoa bo boholo ho ntlafatso ea theknoloji le ntlafatso e tsoelang pele, haholo-holo Tip SPE, li-sieves mahala panel inlaying SPE, 96-384-hole plate SPE e nang le litokelo tse ikemetseng tsa thepa ea mahlale, e tlatsang lekhalo Chaena le ho fihla boemong ba maemo a lefats'e, ho bonahatsa. melemo e ikhethang ea B&M Life Science lebaleng la SPE.

Melemo ea sehlahisoa:

★Ho bonolo ho sebetsa, tlas'a ts'ebetso ea matla a khoheli a tlhaho a ka finyella mefuta e mengata ea lebelo le ho ikatisa hantle

★E ka boloka litšenyehelo tsa lisebelisoa le lisebelisoa haholo ntle le SPE le lisebelisoa tsa vacuum

★sorbent e hloekileng ntle le tšitiso ea bokamorao bo se nang letho

★sekhahla se phahameng sa 10 ~ 100ppm se ne se le maemong a matle ka ho fetesisa a 95% ~ 105%

★Ka matla a maholo a papatso, e phahametse mefuta e meng ea likholomo tsa SPE naheng ea China

★boleng bo tsitsitseng ba sehlahisoa, ho ikatisa hantle, ho kheloha ha maemo ho lekanyelitsoeng (RSD) <5%

★Ha e tšabe kholomo e omileng. Phallo e omileng le e metsi e ts'oana ka har'a mefuta ea liphoso, ho kheloha ho tloaelehileng (RSD) <0.05%

★lihlahisoa tsa rona li ka ho feletseng boemong ba boleng bo lekanang le metsi /Agilent/ Supelco le lik'hamphani tse ling

★Sekhahla sa ts'ebetso ea litšenyehelo tsa lihlahisoa tsa rona ke se seng sa tse ntlehali lefatšeng

Kopo:

★E sebelisoa haholo mobung;Oli;Makeli a 'mele (plasma/moroto, joalo-joalo); Lijo joalo-joalo.

Boitlamo ba boleng:

★Netefatsa hore sehlahisoa se seng le se seng sea tšoaneleha, amohela litekanyetso tse thata tsa taolo ea boleng, etsa tlhahlobo ea batch

★Etsa bonnete ba hore sehlahisoa se seng le se seng ha se na tšitiso e se nang letho, tekanyo ea ho hlaphoheloa e molemo ho feta melao ea naha, ho fihla boemong bo phahameng ka ho fetisisa ba lihlahisoa tse tšoanang.

Boitlamo ba tšebetso:

★Fana ka tšehetso ea litsebi mahala mahala

Classification ea kholomo ea SPE

Polymer matrix letoto la SPE

Ka polymer e chitja e le adsorbent, boholo ba karolo ea sorbent bo tšoana ho feta, sekhahla sa phallo ea kholomo ea SPE se tsitsitse ho feta, e leng kholomo ea khale ea polymer ka laboratoring, letoto lohle la lihlahisoa le sebelisitsoe haholo tlhahlobong ea lijo. Lihlahisoa li lekana le Waters' HLBS, MAX's le MCX's.

Silica gel matrix letoto la SPE

Classic silica gel matrix SPE column, amorphous / spherical adsorbent, e nang le ts'ebetso e phahameng ea litšenyehelo le phihlelo e ntle ea mosebedisi ka tšepo ea bareki.Lihlahisoa li sebelisoa haholo ho hlahloba lik'hemik'hale.

Keletso ea ho ntša / tlhoekiso / ho tsepamisa mohopolo oa SPE

ke sesebelisoa sa phala ea lerumo se nang le ts'ebetso e kentsoeng bakeng sa disampole tsa ho ntša / tlhoekiso ea ntlafatso ea likokoana-hloko kapa Tip of SPE, sepheo sa sehlahisoa sa pipetting spear top eketsa C4 / C18 / silicon powder/magnetic beads/ProtingA (G) agarose gel sorbent, e sebelisetsoang primer/genomic DNA plasmid PCR products/peptide protein/antibodies to target product/filter/extraction/dessalting/purification/enrichment.

Letoto la lipoleiti tsa 96/384-hole SPE

The 96/384-well plate series SPE e ntlafalitsoeng ka ho khetheha bakeng sa mohlala o phahameng oa ho etsa pretreatment.Qetella mokhoa oa ho etsa mohlala pele ho ts'ebetso ka mochini oa orifice oa k'hamphani kapa mochine oa mosebetsi o itekanetseng.

Letoto le ikhethileng la ho lemoha SPE

Kholomo e ikhethang ea ho daea ea Azo: khetha setlala sa diatomite sa Ultra-pure; Theknoloji e khethehileng ea taolo ea sekhahla sa phallo ea sieve-plate, ka tlhahlobo ea litsi tsa naha le kantle ho naha, phepelo ea OEM.

Ho phaella moo, ho na le graphitized carbon black, acid alkali neutral alumina, coconut shell activated carbon, honey discovery column, leather decolorization column, plasticizer discover column……SPE column, more SPE products please ask.

Mehato (nka mohlala oa Tip SPE) :

Ts'ebetso e fapane hanyane ho latela mokhoa oa ho boloka sorbent ka adsorption ea lihlahisoa tse lebisitsoeng kapa litšila.

1. The sorbent adsorbs sehlahisoa se lebisitsoeng, ho tlosa litšila le ho qoba sehlahisoa se lebisitsoeng

Molao-motheo:

ho kenya ho hloekisoa ha mohlala, ho lahla ho hlatsoa, ​​​​lution

Mofuta ona oa ts'ebetso e tiileng ea ho ntša mokhahlelo ka kakaretso o na le mehato e mene:

(1) activation - ho tlosoa ha litšila ho Tip SPE le ho hlahisa tikoloho e itseng ea solvent;

(2) sampuli ea mojaro - qhala sampole ka har'a solvent e itseng, sebelisa pipette ho hema Tip SPE le ho boloka likarolo ho Tip;

(3) leaching - boholo ba ho tlosa litšila le lihlahisoa tse ling tseo e seng tsa sepheo;

(4) elution - tlhahiso le pokello ea sehlahisoa se shebiloeng se nang le molumo o monyenyane oa solvent.

2. The sorbent adsorbs litšila le ho tlosa sehlahisoa se lebisitsoeng

Molao-motheo:

Adsorb litšila holim'a sampuli le ho leleka sehlahisoa se lebisitsoeng

Mofuta ona oa ts'ebetso e tiileng hangata o na le mehato e meraro:

(1). Kenya tshebetsong — tlosa ditshila ho Tip SPE mme o hlahise tikoloho e itseng ya motswako.

(2) loading sampole — inhale Tip SPE with pipette ebe butle-butle u e bululela kantle. Ka nako ena, boholo ba metsoako e shebiloeng e tla fefoloa ka tharollo ea motheo ea sampole, 'me litšila li tla bolokoa ho Tip. Ka hona, mehato e lokela ho qala ho bokella.

(3) Elution - ho hlahisa likarolo tse nang le molumo o monyenyane oa solvent le ho bokella, ho kopanya mokelikeli oa pokello.

Litlhaloso tse ling kapa litlhophiso tsa motho ka mong, rea u amohelabareki bohle ba bacha le ba khale ho botsa, ho buisana ka tšebelisano, batla nts'etsopele e tšoanang!