I-QuEChERS (Ishesha, Ilula, ingabizi, Iyasebenza, Imangelengele futhi Iphephile) emhlabeni jikelele eminyakeni yamuva nje ithuthukise amasu akamuva asheshayo okulungiselela amasampula okutholwa kwemikhiqizo yezolimo. Yasungulwa okokuqala ngu-M Anastassisdes womnyango wezolimo wase-United States (2003), futhi ngemva kokuqinisekisa nokuthuthukiswa okubanzi, indlela ye-QuEChERS yabekwa phambili ngokusemthethweni. Izindlela zokulungiselela ze-QuEChERS ze-SPE pesticide residues zithuthukisa inguqulo, ifana nomphumela wokuhlanzwa kwe-SPE, kodwa isinyathelo sokucubungula sifushane kakhudlwana, sinezici zokonga isikhathi, ukusebenza kahle okuphezulu, umnotho futhi kancane kancane samukelwe ukuhlaziya okuvamile kwabasebenzi. Ngokusebenzisa ikhithi ye-QuEChERS, izinyathelo ezimbalwa kuphela ezidingekayo ukuze kuqedelwe ukulungiswa kwesampula kokuhlaziywa kwezibulala-zinambuzane ezinezigaba eziningi nezinsalela eziningi.

Umkhiqizo wochungechunge lwe-BM – Q uyisinyathelo esingcono kakhulu se-Shenzhen BM Life Science esisekelwe ekuthuthukisweni kwemikhiqizo ecutshungulwa ngokushesha ye-QuEChERS, uchungechunge lwesampula lungasetshenziswa ukuthola izinsalela zezibulala-zinambuzane, ukutholwa kwezinsalela zemithi yezilwane, izithasiselo zokudla, ukutholwa kanye neminye imikhakha. Lolu hlobo lomkhiqizo lungasetshenziswa izinhlobo eziningi, eziningi ezahlukene ze-adsorbent, njenge-BM - GCB depigmentation, BM - PSA ukususa amaprotheni nama-organic acid, BM - NH2 ukususwa kwe-organic acid, BM - WCX purify alkaline substances, BM - C18 susa amafutha nezinye izinto eziphilayo, i-alumina isusa amafutha, i-anhydrous magnesium sulfate ukususwa komswakama. kungasiza ekukhipheni kangcono imithi.Izindlela ezihlukahlukene zokuhlanganisa zingathathwa ukuze zivumelane nezidingo zokwelashwa kwangaphambili ezihlukene.

Izici zochungechunge lwe-BM-Q:
★Izinga eliphezulu lokululama kwezidakamizwa:izinga lokululama elenelisayo kweziningi zezibulala-zinambuzane ezitholakala endaweni epholile.
★Ibanga elibanzi lezinhlelo zokusebenza:ukuze kuhlaziywe izinhlobonhlobo zamasampuli.
★Inqubo ibe lula:indlela ilula, izinyathelo zokusebenza zincane, futhi umphumela wezici zomuntu ngokunemba kwendlela ungancishiswa, kuyilapho ukusebenza kahle kwelabhorethri kungathuthukiswa futhi ukuhamba komsebenzi welabhorethri kungathuthukiswa.
★Imiphumela ithembekile:izinto zokusetshenziswa eziwumgogodla zivela kumphakeli ofanayo, futhi ukungaguquguquki nokuzinza kokuhlosiwe kunokwethenjelwa kakhulu.
★Ukuvikelwa kwemvelo komkhiqizo:ukusetshenziswa kwe-solvent kuncane, ukuvikelwa kwemvelo okuhlanzekile, ngemuva kokwengeza i-solvent, kungavala isitsha ngokushesha, kunciphise ukulimala ku-opharetha.

Incazelo yenqubo:

Ukucubungula isampula:

Ukuqinisekisa ukufana kwesampula.Ngaphambi kokusetshenziswa, amasampula aqinile njengezithelo nemifino kufanele aqoshwe futhi ahlanganiswe ne-homogenated, futhi isampula yemifino nezithelo ngokuvamile i-10-15g, kanye ne-homogenation yomshini we-high speed tissue homogenation (RPM 11000). r/min-24000 r/min).

Isinyathelo 1: ukukhishwa kwesampula:

Inani elilinganisiwe lesampula eliphukile likushubhu yokukhipha i-BM-Q, yengeza izinyibilikisi zemvelo ezifanele kushubhu ye-BM-Q yokukhipha, futhi i-homogenizing noma i-ultrasonic extraction isetshenziselwa ukuhlanza uketshezi.

Isinyathelo sesi-2: ukuhlanzwa

Ngokusho kwesampula, khetha ipayipi lokuhlanza le-BM-Q elifanele, bese wengeza okukhiphayo eshubhuni yokuhlanza, xukuza kahle futhi uhlanganise kahle, futhi ubeke i-centrifuge ukugxila koketshezi noma ukutholwa kwesampula okuqondile.Ngemva kokuhlanzwa kweshubhu elihlanzekile, i-matrix eyinkimbinkimbi ingakwazi qhubeka uhlanza uketshezi lokuhlanza ukuze uzuze umphumela omuhle wokuhlanza.

Amanothi:

Lapho isampula ijoyina umugqa wokuhlanza, i-anhydrous magnesium sulfate ingase ibe nomkhuhlane kanye nokuvela kwegesi, Iziphakamiso zegagasi lokudlidliza ngemva kwesikhathi esithile ngemva kokuvula ishubhu le-centrifuge elichithiwe libe yigagasi lokudlidliza kanye nokunye ukusebenza.

Uma amanzi aqukethe inani elikhulu lamanzi engezwa epayipini lokuhlanza eliqukethe i-magnesium sulfate, sicela wengeze i-solvent ngaphambi kokwengeza isampula ukuvimbela umphumela we-caking wesampula kanye ne-magnesium sulfate.

Sicela unake ukubekwa uphawu komkhiqizo.Lapho umkhiqizo usuvutshiwe, ungavalwa esikhwameni esivalwe kahle noma ugcinwe endaweni eyomile noma engenisa umoya.

Izinkinga ezikhona ngendlela yokuqala ye-QuEChERS:

Ezindaweni ezine-asidi (iwolintshi), izinga lokutholwa kwesibulala-zinambuzane esine-alkali liphansi;Ngisho ku-matrix engathathi hlangothi, izibulala-zinambuzane ezizwela i-alkali ziyancipha.

Izixazululo:

Enqubweni yokuhlaziya i-acidic medium (iwolintshi) ukujoyina ingxube ye-HAC ne-NaAc ekhishwe, ingagcina uhlelo lwesisombululo se-pH phakathi kuka-4 kuya ku-5, ukuze kuxazululwe inkinga yokubuyiswa kwe-alkaline pesticide kuyathinteka.

BM-QuEChERS Oda Ulwazi
     Indlela yokulungiselela isampula, ehlongozwe u-Anastassiades ngo-2003, isetshenziswe kakhulu ekutholeni ukudla, amasampula amanzi emvelo kanye nokungcola komhlabathi.Imikhiqizo yenkampani yethu iyasebenza emnyangweni wezolimo waseShayina, i-NY/T, i-AOAC kanye ne-eu EN.

Lolu chungechunge lwemikhiqizo lungakwazi ukubona ngokugcwele ukusatshalaliswa okuzenzakalelayo, ukulayishwa nokupakishwa kwempushana, kubone ukuzenzela, isikali nokukhiqizwa ngobuningi, futhi kwamukele ukwenza ngokwezifiso komuntu siqu.

Yamukela amakhasimende amasha namadala ukuze ubuze, uxoxisane ngokubambisana futhi ufune intuthuko efanayo!