I-Nucleic Acid Extraction System (I-Nucleic Acid Extraction System) iyinsimbi esebenzisa ama-reagents e-nucleic acid ahambisanayo ukuze iqedele ngokuzenzakalelayo ukukhipha isampula ye-nucleic acid. Isetshenziswa kakhulu ezikhungweni zokulawula izifo, ukuxilongwa kwezifo zasemtholampilo, ukuphepha kokumpontshelwa igazi, ukuhlonza i-forensic, ukuhlola amagciwane emvelo, ukuhlola ukuphepha kokudla, ukufuywa kwezilwane kanye nocwaningo lwebhayoloji yamangqamuzana neminye imikhakha.
1. Indlela yokumunca, eyaziwa nangokuthi indlela yepayipi, iwukukhipha i-nucleic acid ngokuvala ubuhlalu kazibuthe nokudlulisa uketshezi. Ngokuvamile, ukudluliswa kwenziwa ngokulawula ingalo yerobhothi ngohlelo lokusebenza. Inqubo yokukhipha imi kanje:

I-1) I-Lysis: Engeza isisombululo se-lysis kusampula, futhi uqaphele ukuxuba nokuphendula okugcwele kwesisombululo sokusabela ngokunyakaza komshini nokushisa, amaseli akhishwa, futhi i-nucleic acid ikhishwa.

2) I-Adsorption: Engeza ubuhlalu obuzibuthe kusampula ye-lysate, xuba kahle, futhi usebenzise ubuhlalu obuzibuthe ukuze ube nokuhambisana okuqinile kwama-nucleic acid ngaphansi kukasawoti omningi ne-pH ephansi ukuze ukhangise ama-nucleic acid. Ngaphansi kwesenzo sensimu yamagnetic yangaphandle, ubuhlalu kazibuthe buhlukaniswa nesixazululo. , sebenzisa ithiphu ukuze ukhiphe uketshezi futhi ululahle ethangini likadoti, futhi ulahle ithiphu.

3) Ukugeza: Khipha indawo kazibuthe yangaphandle, faka ithiphu elisha bese wengeza isigcinalwazi sokuwasha, xuba kahle ukuze ususe ukungcola, futhi ukhiphe uketshezi ngaphansi kwesenzo senkundla kazibuthe yangaphandle.

4) I-Elution: Susa inkambu kazibuthe yangaphandle, buyisela ithiphu elisha, engeza isigcinalwazi se-elution, hlanganisa kahle, bese uhlukanisa i-nucleic acid eboshiwe kubuhlalu kazibuthe ukuze uthole i-nucleic acid ehlanzekile.
2. Indlela yebha kaMagnetic

Indlela yenduku kazibuthe ibona ukuhlukaniswa kwama-nucleic acid ngokulungisa uketshezi nokudlulisa ubuhlalu kazibuthe. Umgomo kanye nenqubo kuyafana naleyo yendlela yokumunca, kodwa umehluko yindlela yokuhlukanisa phakathi kobuhlalu kazibuthe noketshezi. Indlela yebha kazibuthe iwukuhlukanisa ubuhlalu kazibuthe kusukela kumfucumfucu ngokusebenzisa i-adsorption yentonga kazibuthe iye kubuhlalu kazibuthe, bese ibufaka oketshezini olulandelayo ukuze kubonakale ukukhishwa kwe-nucleic acid.