Ukuhlukaniswa nokuhlanzwa kwamaprotheni kusetshenziswa kakhulu ocwaningweni nasekusetshenzisweni kwe-biochemistry futhi kuyikhono elibalulekile lokusebenza. I-SCG Protein Purification System Company-Saipu Instrument ihlanganise ukuhlukaniswa okungafanele nokuqukethwe okuhle kokuhlukanisaamaprotheniukuhlanzwa kwawo wonke umuntu. Iseli elijwayelekile le-eukaryotic lingaqukatha izinkulungwane zamaphrotheni ahlukene, amanye acebe kakhulu kanti amanye aqukethe amakhophi ambalwa kuphela. Ukuze ufunde iphrotheni ethile, kuyadingeka kuqala ukuhlanza amaprotheni kwamanye amaprotheni nama-molecule angewona amaprotheni.

Ukuhlukana okuqinile

Lapho ukukhishwa kwamaprotheni (ngezinye izikhathi kuxutshwe nama-nucleic acid, ama-polysaccharides, njll.) kutholakala, isethi yezindlela ezifanele ziyakhethwa ukuze kuhlukaniswe oyifunayo.amaprothenikokunye ukungcola. Ngokuvamile, lesi sinyathelo sokuhlukanisa sisebenzisa izindlela ezifana nokukhipha usawoti, ukuqoqwa kwephuzu le-isoelectric kanye nokuhlukaniswa kwe-organic solvent. Lezi zindlela zibonakala ngokulula kanye nomthamo omkhulu wokucubungula, ongasusa ukungcola okuningi futhi ugxilise isisombululo samaprotheni. Okunye okukhishwe kwamaprotheni kukhulu ngevolumu futhi akufanelekile ukugxiliswa ngokunqwabelana noma ukukhipha usawoti. Ungakhetha i-ultrafiltration, i-gel filtration, okomisa i-vacuum eqandisiwe noma ezinye izindlela zokugxilisa ingqondo.

Ukuhlukana okuhle

Ngemuva kokuhlukaniswa okuqinile kwesampula, ivolumu ngokuvamile iba mncane, futhi ukungcola okuningi kususiwe. Ukuze uthole ukuhlanzwa okwengeziwe, izindlela zechromatography ngokuvamile zihlanganisa ukuhlungwa kwejeli, i-ion exchange chromatography, i-adsorption chromatography, kanye ne-affinity chromatography. Uma kunesidingo, ungaphinda ukhethe i-electrophoresis, kuhlanganise ne-zone electrophoresis, isethi ye-isoelectric point, njll njengenqubo yokugcina yokuhlanza. Indlela esetshenziswa ekuhlukaniseni izinga lokuhlukanisa ngokuvamile incane ekuhleleni, kodwa inokulungiswa okuphezulu.

I-Crystallization iyinqubo yokugcina yokuhlukaniswa kwamaprotheni kanye nokuhlanzwa. Nakuba inqubo ye-crystallization ingaqinisekisi ukuthi amaprotheni kufanele afane, kuphela lapho iphrotheni ethile inenzuzo kwisixazululo sokwenza i-crystal. Inqubo ye-crystallization ngokwayo ihambisana nezinga elithile lokuhlanzwa, futhi i-recrystallization ingasusa inani elincane lamaprotheni aphikisiwe. Kusukela denaturedamaprotheniengakaze itholakale ngesikhathi senqubo ye-crystallization, i-protein crystallization ayiyona nje isibonakaliso sokuhlanzeka, kodwa futhi isiqondiso esinamandla sokunquma ukuthi umkhiqizo usesimweni sawo semvelo.