------" I-Ultrafiltration & Microfiltration " Izinhloso eziningi, ibona ukushintshwa kwasekhaya!
I-BM Life Science,I-Ultrafiltration Centrifugal Tube (Isihlungi Se-Centrifugal)Imikhiqizo yochungechunge
Iqukethe ishubhu lokuhlunga elingaphakathi (elinolwelwesi) + ishubhu le-centrifuge langaphandle, elingahlanza ngokushesha nangempumelelo futhi ligxilise kuze kufike ku-15ml wamasampula ezinto eziphilayo. Idizayini ehlukile eqondile kanye nendawo yokuhlunga ekhulisiwe inganikeza umthamo wokucubungula isampula esheshayo kanye nezinga eliphezulu lokutakula isampula (ngokuvamile ≥90% ihlanza isixazululo sangempela), kuyilapho kugcinwa ukuhlunga okumaphakathi nendawo yokugxilisa ingqondo ukuze kugcinwe umsebenzi wamaprotheni kanye ne-Conformation.Umklamo oqondile ohlukile ungahlukanisa i-solute futhi inciphise kakhulu ukungcoliswa kwe-membrane yesihlungi okubangelwa i-centrifugation elandelayo. Ngokulungiselela indawo yokumisa isihlungi esibonakalayo kudivayisi yesihlungi, singavimbela isihlungi se-centrifugal ukuthi somele isampula noma sibangele ukulahleka kwesampula ngenxa yokuzungezisa kakhulu. . Izinhlobonhlobo zezihlungi ze-ultrafiltration molecular weight cutoff (MWCO) centrifugal zisebenzisa ubumsulwa obuphezulu, i-adsorption ephansi ye-PES kanye nolwelwesi lwe-RCE, olunezinga eliphansi kakhulu lokubopha amaprotheni ne-nucleic acid.
I-BM Life Science, ukukhiqizwa kwamakamelo ahlanzekile kuzo zonke izixhumanisi zeI-Ultrafiltration Centrifugal Tube (Isihlungi Se-Centrifugal), ukusebenza komugqa wokuhlanganisa, ukuphathwa okugcwele kwe-ERP, imikhiqizo ehlanzekile kakhulu, ayikho i-DNase/RNase, ayikho i-PCR inhibitors, akukho mthombo wokushisa. I-BM Life Science, bonke osayizi beI-Ultrafiltration Centrifugal Tube (Isihlungi Se-Centrifugal)imikhiqizo yochungechunge yenziwe ngokwezifiso, uchungechunge lwemikhiqizo luzinzile ngamaqoqo anomehluko omncane phakathi kwamaqoqo kanye nekhwalithi ephezulu. Ingashintshwa ngemikhiqizo yasekhaya futhi ithunyelwe e-Japan, eNingizimu Korea, eYurophu nase-United States. Kusetshenziswa kakhulu ekuhlolweni okujwayelekile kokuhlukaniswa kwesampula nokuhlunga.
Isicelo
1 UkuhlungaUkushintshwa kwe-buffer, ukuhlanzwa, ukugxilisa ingqondo, kanye nokukhishwa kukasawoti emanzini kwama-antigen, amasosha omzimba, ama-enzyme, namanye amaprotheni, kanye namasampula ebhayoloji anjengama-nucleic acid nama-microorganisms, ukukhishwa kukasawoti ezingxenyeni ze-chromatographic, ukuhlukaniswa kwama-molecule ebhayoloji esikweni lezicubu noma izixazululo ze-cell lysis, ukususwa. yeziqalo, izixhumi, noma omaka bemolekyuli kusukela kungxube yokusabela, ukugxiliswa kwegciwane noma ukuhlukaniswa, kanye nokuhlukaniswa okungenamsoco kwezingxube ze-molecule yebhayoloji.
2 Ukuhlunga okuncaneihlukanisa ama-nucleic acid namaprotheni kuma-gel, amasampula ayahlungwa futhi acaciswe, futhi izinhlayiya ezicutshunguliwe noma ubuhlalu buqoqwa futhi bugezwe.
Isici Somkhiqizo
★Isilinganiso sokuhlushwa okuphezulu, esingafinyelela kalula izikhathi ezingama-80-100 zokuhlushwa;
★Ijubane lokuhlushwa lesampula liyashesha, futhi isikhathi sokuhlushwa esijwayelekile imizuzu eyi-10-60. Iphinde ibe ne-anti-drying lock lock ukugwema ukulimala kwesampula ngenxa ye-centrifugation eyeqile;
★ Izinga lokutakula isampula liphezulu futhi lingafinyelela izinga lokutakula elingaphezu kuka-90%;
★ I-adsorption ephansi, ulwelwesi lwe-PES/RC nomklamo oshelelayo wodonga lwangaphakathi kune-adsorption yamaprotheni ephansi kakhulu kanye nezinga lokubopha le-biomolecule;
4ml/15ml,15ml/50ml
★ Ukucaciswa okuphelele, amashubhu e-ultrafiltration centrifuge anemiqulu emibili engu-4ml/15ml kanye no-15ml/50ml, anobukhulu obuhlukahlukene bembotshana, atholakala ku-3KD, 5KD, 10KD, 30KD, 50KD kanye no-100KD ukuze kuhlangatshezwane nezidingo ezihlukene zokunqanyulwa kwesisindo samangqamuzana;
★ Ukusebenza okulula, yonke inqubo yokuhlukanisa nokuhlunga ingaqedwa ngeI-Multifunctional Centrifuge, kulula ukusebenza;
★ Umkhiqizo uhlanzekile, zonke izixhumanisi zikhiqizwa ekamelweni elihlanzekile, ukusebenza komugqa womhlangano, ukuphathwa okugcwele kwe-ERP, umkhiqizo u-ultra-pure, akukho DNase / RNase, akukho inhibitor ye-PCR, akukho mthombo wokushisa;
★ Ukuchazwa okuphezulu, akukho ingemuva kanye ne-auto-fluorescence, futhi ayithinti imiphumela yokuthola;
★ Ukusebenza okuthembekile, ukubekwa uphawu okuhle, futhi akuphenduli ngamasampula okuhlola, imiphumela yokuhlola inokwethenjelwa, futhi ingamelana nezinga lokushisa eliphakeme elingu-100 ℃!
★ It has anhlobonhlobo izicelo. Ngezinhlelo zokusebenza ezahlukene, izinhlobo ezimbili zeI-Ultrafiltration Centrifugal Tube (Isihlungi Se-Centrifugal)imikhiqizo yochungechunge, i-4ml/15ml kanye ne-15ml/50ml yethulwa ukuze ihlangabezane nezidingo ezihlukene zamakhasimende, ukwamukela ukwenziwa ngokwezifiso nokuthuthukiswa kokusebenza!
Oda Ulwazi
| Ikati.No | Igama | Ukucaciswa | Incazelo | Ama-PC/pk |
| UCT015RCE004003 | I-Ultrafiltration Centrifugal Tube | 4/15ml, 3kD | , I-RCE Membranes | 15 ama-pcs/isikhwama |
| UCT015RCE004010 | I-Ultrafiltration Centrifugal Tube | 4/15ml, 10kD | Amalungu e-RCE | 15 ama-pcs/isikhwama |
| UCT015RCE004030 | I-Ultrafiltration Centrifugal Tube | 4/15ml, 30kD | Amalungu e-RCE | 15 ama-pcs/isikhwama |
| UCT015RCE004100 | I-Ultrafiltration Centrifugal Tube | 4/15ml, 100kD | Amalungu e-RCE | 15 ama-pcs/isikhwama |
| UCT050RCE015003 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 3kD | Amalungu e-RCE | 24 ama-pcs/isikhwama |
| UCT050RCE015010 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 10kD | Amalungu e-RCE | 24 ama-pcs/isikhwama |
| UCT050RCE015030 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 30kD | Amalungu e-RCE | 24 ama-pcs/isikhwama |
| UCT050RCE015100 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 100kD | Amalungu e-RCE | 24 ama-pcs/isikhwama |
| UCT050PES015005 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 5kD | PES Membranes | 12 ama-pcs/isikhwama |
| UCT050PES015010 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 10kD | PES Membranes | 12 ama-pcs/isikhwama |
| UCT050PES015030 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 30kD | PES Membranes | 12 ama-pcs/isikhwama |
| UCT050PES015050 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 50kD | PES Membranes | 12 ama-pcs/isikhwama |
| UCT050PES015100 | I-Ultrafiltration Centrifugal Tube | 15/50ml, 100kD | PES Membranes | 12 ama-pcs/isikhwama |
| Idatha ye-CF050PES015C010 | Isihlungi Se-Centrifugal | 15/50ml, 0.1um | 0.1um, PES Membranes | 12 ama-pcs/isikhwama |
| I-CF050PES015C020 | Isihlungi Se-Centrifugal | 15/50ml, 0.2um | 0.2um, PES Membranes | 12 ama-pcs/isikhwama |
| I-CF050PES015C045 | Isihlungi Se-Centrifugal | 15/50ml, 0.45um | 0.45um, PES Membranes | 12 ama-pcs/isikhwama |
| UCT** | I-Ultrafiltration Centrifugal Tube | Ukwenza ngokwezifiso | Ukwenza ngokwezifiso | ** ama-pcs/isikhwama |
| CF** | Isihlungi Se-Centrifugal | Ukwenza ngokwezifiso | Ukwenza ngokwezifiso | ** ama-pcs/isikhwama |
I-BM Life Sciences, i-Ultrafiltration Centrifugal Tube (Centrifugal Filter) uchungechunge lwamapharamitha womkhiqizo nedatha yokuhlola
Ukusebenza kochungechunge lwe-Ultrafiltration Centrifugal Tube (Centrifugal Filter) kanye nedatha yokuhlola
I-Ultrafiltration Centrifugal yamasampuli:
5 KD 0.25mg/ml Cyt C;
10 KD1mg/ml OVA;
30/50 KD 1mg/ml BSA;
100 KD 1mg/ml IgG.
Izimo zokuhlola: i-rotor evundlile, amandla e-centrifugal 4000×g, 8℃, ivolumu yokuqala engu-15ml.
I-Ultrafiltration Centrifugal yamasampuli:
5 KD 0.25mg/ml Cyt C;
10 KD 1mg/ml OVA;
30/50 KD 1mg/ml BSA;
100 KD 1mg/ml IgG.
Izimo zokuhlola: i-angle rotor engashintshi, amandla e-centrifugal 5000×g, 8℃, ivolumu yokuqala engu-12ml.
Izilinganiso ezijwayelekile zokunqatshelwa kwamazinga esisindo samangqamuzana amaprotheni (4000g x 15ml ivolumu yokuqala yerotha enyakazayo noma ivolumu yokuqala engu-5000g x 12ml ye-engeli engaguquki)
| izilinganiso zesisindo se-molecular/concentration | isisindo samangqamuzana | Ishubhu ye-NMWL Ultrafiltration | amazinga okunqatshwa % Setha ibhakede | Amazinga okunqatshwa % i-engeli engaguquki | isikhathi se-centrifugation |
| I-vitamin B-12 | 1350 | 3K | ＜25 | ＜25 | 60 |
| (0.25mg/ml) I-Cytochrome C | 12400 | ＞95 | ＞95 | 60 | |
| (1mg/ml) α-Chymotrypsinogen | 25000 | ＞95 | ＞95 | 60 | |
| B-12 (0.2mg/ml) ivithamini | 1350 | 10K | ＜5 | ＜5 | 30 |
| 0.25mg/ml) I-Cytochrome C | 12400 | ＞95 | ＞95 | 30 | |
| (1mg/ml) α-Chymotrypsinogen | 25000 | ＞95 | ＞95 | 30 | |
| uvithamini B-12 (0.2mg/ml) | 1350 | 30K | ＜5 | ＜5 | 20 |
| I-Cytochrome C 0.25mg/ml) | 12400 | ＜10 | ＜10 | 20 | |
| I-Ovalbumin (1mg/ml) | 45000 | ＞95 | ＞95 | 20 | |
| I-BSA (1mg/ml) | 67000 | ＞95 | ＞95 | 20 | |
| I-Cytochrome C (0.25mg/ml) | 12400 | 50K | ＜5 | ＜5 | 10 |
| I-Ovalbumin (1mg/ml) | 45000 | 65 | 55 | 10 | |
| I-BSA (1mg/ml) | 67000 | ＞90 | ＞90 | 10 | |
| I-Ovalbumin (1mg/ml | 45000 | 100K | ＜25 | ＜15 | 20 |
| I-IgG (1mg/ml) | 156000 | ＞90 | ＞90 | 20 | |
| I-Thyroglobulin (0.5mg/ml) | 677000 | ＞90 | ＞90 | 20 |
Izinga lokutakula
Amandla okugcina we-15ml ultrafiltration centrifuge tube membrane achazwa yi-molecular weight cutoff (MWCO).
Izisindo zamangqamuzana asolute eduze ne-MWCO zingagcinwa ingxenye kuphela. Ukuze uthole izici ezingcono kakhulu zokugcina,
sicela usebenzise ulwelwesi lwesihlungi se-MWCO enesisindo semolekyuli esingu-1/3 noma ngaphansi kwe-solute.
Ukuqhathaniswa kwesilinganiso sokutholwa komkhiqizo
Ukuqhathaniswa kwamazinga okubuyisela amaprotheni kanye namavolumu afile ezinhlobo ezahlukene zamashubhu e-ultrafiltration
| I-10KD Ukuqhathaniswa kwamazinga okuthola amaprotheni kanye nokufa imiqulu yemikhiqizo ehlukene yamashubhu e-ultrafiltration | |||
| Ibhrendi | (%) Izinga lokutakula | (μl) Ivolumu efile | |
| BM | 1㎎/ml Ukugxila kwamaprotheni | 90 | 87 (Ukugxilisa amaprotheni1㎎/ml) |
| 0.1㎎/ml Ukugxila kwamaprotheni | 83 | ||
| Brand M | 1㎎/ml Ukugxila kwamaprotheni | 93 | 47 (Ukugxilisa amaprotheni1㎎/ml) |
| 0.1㎎/ml Ukugxila kwamaprotheni | 88 | ||
| Brand S | 1㎎/ml Ukugxila kwamaprotheni | 91 | 70 (Ukugxilisa amaprotheni1㎎/ml) |
| 0.1㎎/ml Ukugxila kwamaprotheni | 85 | ||
| Brand K | 1㎎/ml Ukugxila kwamaprotheni | 89 | 186 (Ukugxilisa amaprotheni1㎎/ml) |
| 0.1㎎/ml Ukugxila kwamaprotheni | 77 | ||
| Brand J | 1㎎/ml Ukugxila kwamaprotheni | 83 | |
| 0.1㎎/ml Ukugxila kwamaprotheni | 50 | ||
| Izimo zokuhlola: Amaprotheni e-OVA (isisindo se-molecular 45KD), i-rotor evundlile 4000g | |||
Ukucaciswa kwereferensi yokukhetha amashubhu e-ultrafiltration anesisindo samangqamuzana ahlukene
| ivolumu yokuqala | ＜0.5 ml | ＜2 ml | ＜4 ml | 15ml | |
| Amaprotheni | |||||
| (MW)isisindo semolekyuli | I-MWCO (Da) inqamule isisindo samangqamuzana | ||||
| 6K＜MW＜20K | 3000 | 3000 | 3000 | 3000 | |
| 20K＜MW＜60K | 10000 | 10000 | 10000 | 10000 | |
| 60K＜MW＜100K | 30000 | 30000 | 30000 | 30000 | |
| 100K＜MW＜200K | 50000 | 50000 | 50000 | 50000 | |
| 200K＜MW | 100000 | 100000 | 100000 | 100000 | |
| Ama-nucleic acid anemicu eyodwa nephindwe kabili | |||||
| ubude | I-MWCO (Da) inqamule isisindo samangqamuzana | ||||
| 137-1159bp | 3000 | 3000 | 3000 | 3000 | |
| ama-nanoparticles | |||||
| (DIA) usayizi wezinhlayiyana | I-MWCO (Da) inqamule isisindo samangqamuzana | ||||
| 1.5＜DIA＜3nm | 3000 | 3000 | 3000 | 3000 | |
| 3＜DIA＜5nm | 10000 | 10000 | 10000 | 10000 | |
| 5＜DIA＜7nm | 30000 | 30000 | 30000 | 30000 | |
| 7＜DIA＜10nm | 50000 | 50000 | 50000 | 50000 | |
| 10 nm＜DIA | 100000 | 100000 | 100000 | 100000 | |
Isikhathi sokuthumela: Feb-18-2024
