Izindlela zeukuhlanza amaprotheni:

Indlela yokuhlanzwa kwamaprotheni, ukuhlukaniswa nokuhlanzwa kwamaprotheni, iphrotheni ikhishwa kumangqamuzana okuqala noma izicubu esimweni esincibilikile futhi ihlala esimweni sokuqala semvelo ngaphandle kokulahlekelwa umsebenzi wezinto eziphilayo. Ngenxa yalesi sizathu, impahla kufanele isuse izicubu zezilwane ne-adipose, imbewu kufanele ikhiphe ngisho nokungcoliswa kwejazi lembewu ezintweni ezifana nama-tannins, futhi imbewu kawoyela kufanele isebenzise i-solvent ye-organic ebilisa kancane.

1. Indlela yokuhlanzwa kwamaprotheni, ubukhulu kanye nezindlela ezihlukene zokuhlaziya ukuhlukaniswa: i-dialysis kanye ne-ultrafiltration zisebenzisa lawa maprotheni ukuze adlule emvelweni wama-molecule angakwazi ukudlula ku-membrane ye-semi-permeable; ukuminyana kwe-gradient centrifugation Izinga nokuminyana kwephrotheni phakathi nendawo kuzoba nomthelela omkhulu ezinhlayiyeni. Isivinini sokumisa siyashesha; ukuhlunga kwejeli kuwuhlobo lwekholomu yechromatography.

2. Ukwehlukaniswa komehluko wokuncibilika okusetshenziswayo: i-electro-precipitation ngenxa yokuthi ingqikithi yokushajwa kwama-molecule amaprotheni iku-zero isoelectric point, kunciphisa ukunengwa kwe-electrostatic phakathi kwama-molecule, ukuhlanganisa kalula, kanye nokuncibilika okuncane; isixazululo sikasawoti esisetshenziswa nesixazululo sikasawoti Ukugxila kwandisa noma kunciphisa ukuncibilika kwephrotheni.

3. Inkokhelo ihlukaniswa futhi ihlaziywe ngezindlela ezahlukene, okuqukethwe okuyinhloko kufaka phakathi i-electrophoresis ne-ion exchange chromatography.

4. Izindlela zeukuhlanza amaprotheniI-adsorption ekhethiwe kanye nokwehlukaniswa kwamaprotheni kusebenzisa amandla e-particle adsorption.

5. I-Affinity chromatography isekelwe ekuhlukaniseni ama-ligands. Lesi sici sebhayoloji sivumela ama-molecule wamaprotheni ukuthi abophe ngokuqondile kunokuba ahlangane ngokuhlanganyela kwenye i-molecule ebizwa ngokuthi i-ligand.

6. Indlela yokuhlanza amaprotheni: Indlela yezulu esezingeni eliphansi ye-solvent ye-organic: Sebenzisa izinyibilikisi eziphilayo ezixubana namanzi, njenge-methanol, i-ethanol, ukuze unciphise ukuncibilika kanye nemvula yamaphrotheni amaningi. Le ndlela iphakeme kunokulungiswa kosawoti ngaphandle, kodwa amaprotheni ashintsha kakhulu Uma kulula, kufanele kwenziwe ezingeni lokushisa eliphansi.

Indlela yokuhlanzaukuhlanza amaprotheni:

Indlela yokuhlanza amaprotheni, amaprotheni iyingqamuzana enkulu, kanye nobukhulu obuhlukahlukene be-albumin, kunezindlela ezilula zokuhlukanisa amaprotheni nezinto ezincane ze-molecule, kanye nengxube yamaprotheni ehlukanisiwe. Izindlela eziyinhloko zokuhlukanisa amaprotheni amasayizi amangqamuzana ahlukene i-dialysis, i-ultrafiltration, i-gel filtration, nokuhlukaniswa kwe-centrifugal. I-dialysis kanye ne-ultrafiltration kuvame ukusetshenziswa ezindleleni zokuhlukanisa amaprotheni. Ingxube ye-dialysis ezohlukaniswa ifakwe esikhwameni se-dialysis esenziwe nge-membrane ye-semipermeable, bese icwiliswa kusixazululo se-dialysis ukuze kuhlukaniswe. I-Ultrafiltration inqubo yokudlulisa amanzi namanye ama-molecule amancane kusetshenziswa amandla aphakathi nendawo noma ukucindezela kulwelwesi olungapenyeleka futhi lubambe amaprotheni kulwelwesi olungapenyeleki kancane. Lezi zindlela ezimbili zingahlukaniswa kuma-macromolecules asekelwe kumaprotheni kanye nama-molecule amancane asekelwe kusawoti we-inorganic. Zivamise ukugaywa usawoti, zisetshenziswe ngokuhambisana nendlela yokuncibilikisa usawoti, noma zingethulwa ukuze zikhiphe lezi zindlela ezimbili ngemva kokufakwa kukasawoti kusetshenziswa izixazululo zikasawoti ezingaphili.

1. Ungawahlanza kanjani amaprotheni ngendlela yokukhipha usawoti: Isisekelo sendlela yokukhipha usawoti wukuthi ukuncibilika kweprotheyini esixazululweni sikasawoti esincibilikisiwe kuzokhula ngokwanda kokugxila kukasawoti, kodwa lapho ukugcwala kukasawoti kukhuphukela enanini elithile, umsebenzi wamanzi uzokwanda. Ukuncipha, okungase kubangele ukuthi amanye amaprotheni akhiqize amashaji ngokusebenzisa indawo yesakhiwo samangqamuzana ukuthi anciphe kancane kancane, futhi ulwelwesi lwe-hydration lubhujiswe kancane kancane, olungagcina lubangele amaphrotheni nama-molecule ahlobene ukuthi akhe i-agglomeration kanye nemvula evela kuzixazululo ezahlukahlukene.

2. Indlela yemvula ye-solvent ye-Organic: Kunezizathu ezimbili zokunciphisa ukuncibilika kwamaprotheni kuma-organic solvents: indlela yokuhlanza amaprotheni njengesixazululo sikasawoti ngokuphela kwamanzi; okwesibili, i-dielectric constant of organic solvents kunamanzi ingancishiswa Ku-polar solvents.

3. I-ejenti enisa amaprotheni: Iphrotheni njenge-ejenti edonsayo isebenza kuphela ohlotsheni olulodwa noma olulodwa lwezulu lamaprotheni, ezivamile i-alkaline, amaprotheni, i-lectin, nezinsimbi ezisindayo.

4. Imvula ye-polyethylene glycol: Ungawahlanza kanjani ama-polymer angewona ama-ionic ancibilika emanziniukuhlanza amaprotheni, njenge-polyethylene glycol ne-sodium dextran sulfate ukuze kuncishiswe amaprotheni.