I-Solid phase extraction (SPE) inqubo yokukhipha ngokomzimba ehlanganisa izigaba eziwuketshezi neziqinile. Enqubweni yokukhipha, amandla e-adsorption okuqinile kuya ku-analyte makhulu kunesampula yotshwala kamama. Lapho isampula idlula ku-I-SPEikholomu, i-analyte ikhangiswe endaweni eqinile, futhi ezinye izingxenye zidlula kukholamu nesampula yotshwala kamama. Ekugcineni, i-analyte isuswa nge-solvent efanele Eluted. I-SPE inezinhlobonhlobo zezinhlelo zokusebenza, njengokuhlaziywa koketshezi lwebhayoloji okuhlanganisa igazi, umchamo, i-serum, i-plasma ne-cytoplasm; ukuhlaziywa kokwenziwa kobisi, iwayini, iziphuzo namajusi ezithelo; ukuhlaziywa kanye nokuqapha imithombo yamanzi; izithelo, imifino, okusanhlamvu, kanye nezicubu zezitshalo ezihlukahlukene Izicubu zezilwane; imithi eqinile njengamaphilisi. Ukuhlaziywa kwezinsalela ze-pesticide kanye ne-herbicide ezithelweni, imifino nokudla, ukuhlaziywa kwama-antibiotic kanye nezidakamizwa zomtholampilo, njll.

(1) Khipha ngokucophelela idivayisi yokukhipha isigaba esiqinile bese usibeka ngobumnene ebhentshini lokusebenza.

(2) Khipha ngokucophelela ikhava engaphezulu ye-I-SPEidivayisi (ubambe ngobumnene ukuze ungalimazi ishubhu elincane), faka ishubhu lokuhlola elivamile embotsheni yokwahlukanisa egumbini le-vacuum, bese uvala ikhava eyomile engaphezulu, futhi uqinisekise ukuthi ikhava iqondiswa phansi. Ishubhu eligelezayo kanye neshubhu lokuhlola kuhambelana ngayinye ngayinye, futhi indandatho yesikwele yokuvala yecwecwe lekhava inomsebenzi omuhle wokuvala ngegumbi levacuum. Uma kungelula ukuvala, ingaqiniswa ngebhande lenjoloba ukwandisa ukucinana.

(3) Uma uthenge ukulungiswa okuzimele, kufanele uqale ufake i-valve yokulungisa emgodini wokukhipha wekhava;

(4) Uma ungadingi ukwenza amasampula angu-12 noma angu-24 ngesikhathi, xhuma i-valve enaliti eqinile emgodini wokukhipha ongasetshenzisiwe;

(5) Uma kuthengwa ivalvu yokulawula ezimele, vula iphinifa levalvu yembobo engasetshenziswanga liye endaweni yokuvala evundlile;

(6) Faka igobolondo lesigaba esiqinile sokukhipha imbobo yokukhipha noma imbobo yevalvu yekhava engaphezulu (vula ifindo levalvu elilawulayo endaweni evulekile eqondile); xhuma idivayisi yokukhipha kanye nepompo ye-vacuum nge-hose, futhi uqinise i-valve elawula ukucindezela;

(7) Jova amasampula noma ama-reagents azokhishwa kukholamu yokukhipha, bese uqala ipompo ye-vacuum, bese isampula kukholomu yokukhipha izogeleza ngekholomu yokukhipha ithubhu yokuhlola engezansi ngaphansi kwesenzo sokucindezela okungalungile. Ngalesi sikhathi, izinga lokugeleza kwe-liquid lingashintshwa futhi lilawulwe ngokulungisa i-valve yokunciphisa ingcindezi.

(8) Ngemva kokuba uketshezi olukwishubhu yenaliti lumpontshwa, vala iphampu yevacuum, khipha ikholomu yokucebisa ocingweni, khipha ikhava engaphezulu yocingo, khipha ishubhu lokuhlola bese ulithela.

(9) Uma ungafuni ukusebenzisa ishubhu lokuhlola ukuze uxhume uketshezi, ungakhipha indawo yokubeka ishubhu yokuhlola, uyifake esitsheni esinosayizi ofanele, bese uyikhipha ngemva kokukhipha okokuqala.

(10) Faka ishubhu lokuhlola elihlanzekile ocingweni, vala ikhava, faka i-cartridge ye-SPE, engeza isincibilikisi esidingekayo epayipini yenaliti, qala ipompo yokuvala, vala amandla ngemva kokukhishwa koketshezi, bese ukhipha test tube ukusetshenziswa. Ukukhishwa kanye nokulungiswa kwesampula kuqediwe.

(11) Faka ishubhu lokuhlola entweni yokomisa i-nitrogen futhi uhlanze futhi ugxile nge-nitrogen, futhi ukulungiswa sekuphelile.

(12) Lahla okokuncibilikisa epayipini lokuhlola, bese ugeza ishubhu lokuhlola ukuze liphinde lisetshenziswe.

(13) Ukuze wonge izindleko zokusebenzisa i-I-SPEikholomu, ngemva kokusetshenziswa ngakunye, ikholomu ye-SPE kufanele igezwe nge-eluent ukuze kuqinisekiswe izici zokupakishwa kwayo.