Ibhodlela lesampula liyisitsha sokuhlaziya insimbi yento okufanele ihlaziywe, futhi ukuhlanzeka kwayo kuthinta ngokuqondile umphumela wokuhlaziya. Lesi sihloko sifinyeza izindlela ezahlukahlukene zokuhlanza isampula yebhodlela le-chromatographic, futhi ihlose ukuhlinzeka ngereferensi enengqondo yawo wonke umuntu. Lezi zindlela ziqinisekiswe ngabangane nabanduleli. Zinomphumela omuhle wokuwasha ezinsaleni ezincibilika amafutha kanye nezinsalela ze-organic reagent endaweni.ibhodlela lesampula le-chromatography. Ukuhlanzeka kuhlangabezana nezidingo, izinyathelo zokuhlanza zilula, futhi isikhathi sokuhlanza sincishisiwe, futhi inqubo yokuhlanza ihambisana nemvelo.

Sicela uzikhethele ngokwakho ngokusekelwe esimweni sakho selabhorethri!

Njengamanje, ngentshisekelo ekhulayo yekhwalithi yokudla nokuphepha kuzo zonke izigaba zokuphila, ubuchwepheshe bokuhlaziya i-chromatographic buyasetshenziswa kakhulu ekuhlolweni kwekhwalithi yokudla nokuphepha, ikakhulukazi emkhakheni wokuhlolwa komkhiqizo wezolimo, ubuchwepheshe bokuhlaziya i-chromatographic busetshenziswe kabanzi. Ezweni lami, inani elikhulu lemikhiqizo yezolimo (eminye imikhiqizo yamakhemikhali, i-organic acid, njll.) idinga ukuhlolwa nge-chromatography ewuketshezi kanye ne-chromatography yegesi njalo ngonyaka. Ngenxa yenani elikhulu lamasampula, kunenani elikhulu lamabhodlela esampula adinga ukuhlanzwa phakathi nenqubo yokutholwa, okungachithi nje kuphela isikhathi futhi kunciphise ukusebenza kahle komsebenzi, kodwa futhi ngezinye izikhathi kubangela ukuphambuka emiphumeleni yokuhlola ngenxa yokuhlanzeka komshini. amabhodlela esampula ahlanziwe.

IIbhodlela lesampula le-chromatographicngokuyinhloko yenziwe ngengilazi, akuvamile ukuba ipulasitiki. Amabhodlela amasampula alahlwayo ayabiza, ayamosha, futhi adala ukungcoliswa kwemvelo okukhulu. Amalabhorethri amaningi ahlanza amabhodlela esampula futhi aphinde asetshenziswe. Njengamanje, izindlela ezivame ukusetshenziswa ezindaweni zokucwaninga ukuhlanza ibhodlela lesampula ngokuyinhloko ukwengeza impushana yokuwasha, okokuhlanza, i-organic solvent, kanye ne-acid-base lotion, bese ukukhuhla ngeshubhu elincane lokuhlola elenziwe ngokwezifiso. Le ndlela evamile yokukhuhla inamaphutha amaningi. Isebenzisa izihlanzi namanzi amaningi, ithatha isikhathi eside ukuwashwa, futhi ijwayele ukushiya amakhona afile. Uma kuyisampula yebhodlela lepulasitiki, kulula ukushiya amamaki ebhulashi odongeni lwebhodlela langaphakathi, okuthatha inqwaba yabantu. Ku-glassware engcoliswe kakhulu yizinsalela ze-lipid namaprotheni, isisombululo se-alkaline lysis sisetshenziselwa ukuhlanza, futhi imiphumela emihle ifinyelelwa.

Uma uhlaziya amasampula, ukuhlanzwa kwebhodlela lomjovo kubaluleke kakhulu. Ngokwendlela yokugeza i-glassware, indlela yokuhlanza ikhethwa ngokwezinga lokungcola, futhi ayikho imodi ehleliwe. Isifinyezo sendlela:

1. Thela isisombululo sokuhlola ebhodleleni elomile

2. Gxilisa konke ku-95% wotshwala, ugeze kabili nge-ultrasonic futhi uwuthulule, ngoba utshwala bungena kalula ku-vial engu-1.5mL futhi bungaxubana nezinyibilikisi eziningi eziphilayo ukuze kuzuzwe umphumela wokuhlanza.

3. Thela emanzini ahlanzekile, futhi ugeze kabili nge-ultrasonically.

4. Thela amafutha ebhodleleni elomile bese ubhaka ku-110 degrees Celsius amahora angu-1 ~ 2. Ungalokothi ubhake ezingeni lokushisa eliphezulu.

5. Pholisa futhi wonge.