Inqubo evamile yokukhipha isigaba esiqinile imi kanje:

1. Ukwenza i-adsorbent isebenze: Hlanza i-cartridge yesigaba esiqinile sokukhipha nge-solvent efanele ngaphambi kokukhipha isampula ukuze ugcine i-adsorbent imanzi, engakha izinhlanganisela ezihlosiwe noma izinhlanganisela eziphazamisayo. Izindlela ezihlukene zokuvula i-cartridge yesigaba esiqinile zisebenzisa izinyibilikisi ezihlukene:

(1) Izithasiselo ze-polar ezibuthakathaka noma ezingezona ze-polar ezisetshenziswa ekukhishweni kwesigaba esiqinile sesigaba esihlehlisiwe ngokuvamile zihlanjululwa ngesithambisi semvelo esincibilika emanzini, njenge-methanol, bese sihlanjululwa ngamanzi noma ngesixazululo sebhafa. Kungenzeka futhi ukugeza nge-solvent enamandla (efana ne-hexane) ngaphambi kokugeza nge-methanol ukuze kuqedwe ukungcola okukhangisiwe ku-adsorbent kanye nokuphazanyiswa kwayo nenhlanganisela eqondiwe.

(2) I-polar adsorbent esetshenziswa ekukhishweni kwesigaba esiqinile sesigaba esivamile ngokuvamile ikhishwa nge-organic solvent (isampula le-matrix) lapho inhlanganisela eqondiwe ikhona.

(3) I-adsorbent esetshenziswa ekukhishweni kwesigaba esiqinile se-ion-exchange ingagezwa nge-solvent eyisampula uma isetshenziselwa amasampula ezincibilikisi ze-organic non-polar; uma isetshenziselwa amasampula ku-solvents ye-polar, ingagezwa ngezincibilikisi eziphilayo ezincibilika emanzini Ngemva kokugeza, hlambulula ngesisombululo samanzi se-pH efanelekile futhi equkethe ama-solvents athile aphilayo nosawoti.

Ukuze ugcine i-sorbent ku-cartridge ye-SPE imanzi ngemuva kokusebenza nangaphambi kokwengezwa kwesampula, cishe i-1 ml ye-solvent ukuze isebenze kufanele igcinwe ku-sorbent ngemva kokusebenza.

2. Ukulayisha isampula: Thela uketshezi noma isampuli eqinile encibilikisiwe kukhatriji yokukhipha isigaba esiqinile, bese usebenzisa i-vacuum, ingcindezi noma i-centrifugation ukuze wenze isampula ingene ku-adsorbent.

3. Ukugeza kanye ne-elution: Ngemva kokuba isampula ingene ku-adsorbent futhi inhlanganisela ehlosiwe isikhangisiwe, isakhi esiphazamisayo esigcinwe buthaka singagezwa nge-solvent ebuthaka, bese inhlanganisela ehlosiwe ingakhishwa ngesixazululi esinamandla futhi siqoqwe. . Hlambulula kanye Nokukhanya Njengoba kuchaziwe ngaphambili, i-eluent noma i-eluent ingadluliselwa ku-adsorbent ngokusebenzisa i-vacuum, ingcindezi noma i-centrifugation.

Uma i-adsorbent ikhethwa ukuthi ibe ne-adsorption ebuthakathaka noma engekho ku-compound eqondiwe kanye ne-adsorption eqinile ku-compound ephazamisayo, inhlanganisela ehlosiwe ingabuye ihlanjululwe futhi iqoqwe kuqala, kuyilapho i-compound ephazamisayo igcinwa (adsorption). ) ku-adsorbent, bobabili bahlukene. Ezimweni eziningi, inhlanganisela ehlosiwe igcinwa ku-adsorbent, futhi ekugcineni ikhishwe nge-solvent eqinile, evumela kakhulu ukuhlanzwa kwesampula.