I-Fumonisin Affinity Chromatography

Umgomo wokuhlanza wekholomu ekhethekile ye-Fumonisin iyimpendulo yokuzivikela komzimba phakathi kwama-antigen. Amasosha omzimba e-Monoclonal aqukethe ukuhlolwa kwe-Fumonisin agxilwe ekusekelweni okuqinile kwesigaba sekholomu yokuthola. Isampula ekhishwe equkethe ama-Fumonisin isetshenziselwa ukuthola ikholomu ekhethekile ye-Fumonisin, engahlanganiswa ne-antibody ukuze kwakhe i-antigen antibody complex, esuke igezwa ngamanzi ukuze iye kulokho okuhlosiwe. Okokugcina, ukucacisa nge-eluent, qoqa i-eluent, sebenzisa i-HPLC ukuthola okuqukethwe kwe-Fumonisin.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

AmaFumonisin awubuthi besikhunta obukhiqizwa amasikela, okwamanje kukhona okuphuma kokunye okwaziwayo okungu-28, Olunye lwezifundo ezivame kakhulu nezijulile yi-FB1. Kutholakale ukuthi ummbila nemikhiqizo yawo, njengokuphakelwa kwezilwane, kungcoliswa kalula yi-FB1. I-FB1 inamandla kakhulu kumaFumonisin futhi inomthelela omubi kakhulu we-toxicological ezilwaneni eziningi. Ucwaningo luye lwabonisa ukuthi ihhashi elimhlophe le-FB1 lingabangela ukuthamba kwesifo sobuchopho, i-swine pulmonary edema syndrome, ngaphezu kwalokho, lisengakwazi ukufaka umdlavuza we-esophageal kanye nomdlavuza wesibindi, umdlavuza wesisu nezinye izifo, ukufuywa kwezilwane kanye nempilo yabantu. nayo ibalulekile.

Uchungechunge oluyinhloko lwekholomu ekhethekile yokuthola ubuthi be-B&M ukutholwa kwe-immuno-affinity kanye nekholomu ekhethekile ye-FB1 (FB1).Le kholomu ingakhanga ngokukhetha i-vomatoxin B1 (FB1) kusixazululo sesampula, ngaleyo ndlela umphumela wokuhlanza wekholomu ungaqondiswa. , futhi isampula ingahlolwa ngokuqondile yi-HPLC ngemva kokuba ikholomu isihlanziwe.

Isicelo:
Ukolweni; okuphakelayo; amafutha adliwayo, njll.
Izicelo Ezijwayelekile:
Isetshenziselwa ukuhlanzwa kwe-Fumonisin
ama-substrates ayinkimbinkimbi futhi umkhawulo ophansi. Ukuhlaziywa komthamo
ye-TLC/HPLC/GC/LC-MS/EIA;
Ingakwazi ukukala ngekhwalithi nangokwezibalo
inani elisele le-Fumonisin emmbila, ukudla, amafutha adliwayo
namanye amasampula

Oda Ulwazi

Ama-sorbents

Ifomu

Ukucaciswa

Ama-PC/pk

Ikati.No

I-Cartridge yokuthola ama-Fumonisin Ikhatriji 1mL

25

I-FB-IAC0001
I-Cartridge yokuthola ama-Fumonisin   3mL

20

I-FB-IAC0003
Ikholomu engenalutho ye-affinity chromatography   1mL, Izingcezu ezimbili ze-hydrophilic Frits

100

I-ACC001
Ikholomu engenalutho ye-affinity chromatography   3mL, Izingcezu ezimbili ze-hydrophilic Frits

50

I-ACC003

  • Okwedlule:
  • Olandelayo:

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