Ukuhlaziywa kwe-Blot ku-Biopharmaceutical, Medical kanye nezinye Izinkambu

“UHlelo Lweminyaka Emihlanu” lwe-Bioeconomy Development Plan luphakamisa ukuthi i-bioeconomy kufanele iqhutshwe ukuthuthukiswa nenqubekelaphambili yesayensi yezempilo kanye ne-biotechnology, okusekelwe ekuvikelweni, ekuthuthukisweni nasekusetshenzisweni kwemithombo yemvelo, futhi kusekelwe ekudidiyeleni okujulile nokujulile. imithi, ezempilo, ezolimo, ezamahlathi, namandla. , ukuvikelwa kwemvelo, izinto zokwakha kanye nezinye izimboni; kuyacaca ukuthi ukuthuthukiswa kwe-bio-economy kuyisiqondiso esibalulekile sokuhambisana nenkambiso yenguquko esheshayo ye-biotechnology yomhlaba wonke kanye nokuzuza ukuzimela kwezesayensi nobuchwepheshe bezinga eliphezulu. Kuyisinyathelo esibalulekile ukutshala nokwandisa imboni yezokuphila kanye nokukhuthaza ukuthuthukiswa komnotho okuseqophelweni eliphezulu. Ukuhlangabezana nokukhula okusheshayo kwezidingo zempilo kanye nezempilo kanye nokwanelisa ukulangazelela kwabantu impilo engcono kuyisiqinisekiso esibalulekile sokuqinisa ukuvikela nokulawula ubungozi be-biosecurity kazwelonke kanye nokugqugquzela ukwenziwa kwesimanjemanje kohlelo lokubusa lukazwelonke kanye namandla okubusa.

Esabela obizweni lukazwelonke, i-BM izibophezele ekunqobeni ubuchwepheshe bokukhiqiza amafilimu asezingeni eliphezulu futhi kancane kancane ibona ukushintshwa kokungenisa kwezinto ezisetshenziswayo ezinenani eliphezulu emkhakheni wesayensi yezempilo. NgoMeyi 2023, ukukhiqizwa okukhulu kwe-immunochromatography NCulwelwesiitholwe ngempumelelo futhi yasetshenziswa kuma-reagents okuhlonza ngokushesha ahlukahlukene. Njengamanje, ifilimu ye-NC isetshenziswe ekuxilongweni kwe-in vitro yasekhaya, ukuphepha kokudla, ukuhlolwa okusheshayo kwezidakamizwa neminye imikhakha, futhi izuze ukuthunyelwa kwamanye amazwe futhi yancintisana neziqhwaga zamazwe ngamazwe emakethe! Ngemva kokuphothula inkulumo yemakethe yefilimu ye-NC, ngemva kwezinyanga ezimbalwa zocwaningo lobuchwepheshe lwethimba lethu lezobuchwepheshe, liphendula izidingo eziphuthumayo zabasebenzisi emkhakheni wesayensi yempilo yomhlaba wonke ukuze kwehliswe izindleko zezinto ezisetshenziswayo ezinenani eliphezulu, sethule ngempumelelo ukuvala.ulwelwesis, elungele i-biopharmaceuticals, imithi kanye neminye imikhakha. Ukuhlaziywa kwe-Western blotting (Western Blotting, WB)

Isethulo sezici ze-BM Blotting Membranes, : Usayizi wembobo kanye nohlobo lweprotein olusebenzayo elingu-0.1μm olufanele amaprotheni anesisindo sengqamuzana esingaphansi kuka-7kDa 0.22μm afanele amaprotheni anesisindo sengqamuzana esingaphansi kuka-20kDa 0.45μm afanele amaprotheni anesisindo samangqamuzana amakhulu kuno-20kDa Main Izimiso ezibophezela amaprotheni Ugesi omile kanye ne-hydrophobicity Izimo zokudlulisa ezisebenzayo nezindlela zokuthola I-Chemiluminescence Ukutholwa kwe-Fluorescence nge-radiolabeled probe ukudaya okuqondile kwe-Enzyme-linked antibody Inzuzo:

1.Ingemuva eliphansi, ukuzwela okuphezulu

2.Asikho isidingo sokumanzisa i-alcohol reagent

3.Isakhiwo esiyingqayizivele sendawo kanye nezakhiwo zakha isilinganiso esihle kakhulu sesignali-kuya-kumsindo Izinto ezibonakalayo zitholakala emicucu yemvelo, ihambisana nemvelo, futhi ingagcina iphrotheni eboshiwe isebenza isikhathi eside.

Isingeniso sobuchwepheshe bokuhlaziya i-WB Ubuchwepheshe bokuhlaziya i-WB ubuchwepheshe obusetshenziswa kakhulu emkhakheni webhayoloji yamangqamuzana, i-biochemistry, i-immunology neminye imikhakha. Lobu buchwepheshe busebenzisa ukubophezela okukhethekile kwamasosha omzimba kumaphrotheni athile kuzicubu noma amasampula eseli ukuze kuzuzwe ukuhlonza amaprotheni kanye nokuhlaziywa kwenkulumo ngokusekelwe endaweni kanye nokuqina kwebhande lombala, okungukuthi, ukuhlaziywa kwekhwalithi nokulinganisa okuncane. Yahlongozwa okokuqala ngu-Harry Towbin we-Friedrich Miescher Institute e-Switzerland ngo-1979. Sekuyiminyaka engaphezu kwengu-40 edlule futhi isiphenduke indlela yocwaningo lwamaprotheni yakudala kakhulu nesebenzayo.