I-Oligonucleotide (i-Oligonucleotide), ngokuqhelekileyo ibhekisela kumgca we-polynucleotide fragment ye-2-10 i-nucleotide residues edityaniswe ngamabhondi e-phosphodiester, kodwa xa eli gama lisetyenziswa, inani leentsalela ze-nucleotide Akukho migaqo engqongqo. Kwiincwadi ezininzi, iimolekyuli ze-polynucleotide eziqulethe i-nucleotide ezingama-30 okanye ngaphezulu zibizwa ngokuba yi-oligonucleotides. I-Oligonucleotides inokwenziwa ngokuzenzekelayo ngezixhobo. Zingasetyenziswa njenge-DNA synthesis primers, gene probes, njl., kwaye zineendlela ezininzi zokusetyenziswa kuphando lwangoku lwebhayoloji yemolekyuli.

isicelo

I-Oligonucleotides isoloko isetyenziswa njenge-probes ukumisela ubume be-DNA okanye i-RNA, kwaye isetyenziswa kwiinkqubo ezifana ne-gene chip, i-electrophoresis, kunye ne-fluorescence in situ hybridization.

I-DNA eyenziwe yi-oligonucleotide ingasetyenziselwa kwi-polymerization chain reaction, enokuthi ikhulise kwaye iqinisekise phantse zonke iziqwenga ze-DNA. Kule nkqubo, i-oligonucleotide isetyenziswe njenge-primer ukudibanisa kunye ne-label complementary fragment kwi-DNA ukwenza ikopi ye-DNA. .

I-oligonucleotides elawulayo isetyenziselwa ukuthintela amaqhekeza e-RNA kwaye ithintele ukuba iguqulelwe kwiiprotheni, ezinokudlala indima ethile ekumiseni umsebenzi weeseli zomhlaza.