I-Nucleic Acid Extraction System (I-Nucleic Acid Extraction System) sisixhobo esisebenzisa i-reagents ehambelana ne-nucleic acid extraction ukugqiba ngokuzenzekelayo isampuli ye-nucleic acid extraction. Isetyenziswa kakhulu kumaziko olawulo lwezifo, ukuxilongwa kwezifo zeklinikhi, ukhuseleko lotofelo-gazi, ukuchongwa kwe-forensic, uvavanyo lwe-microbial yokusingqongileyo, uvavanyo lokhuseleko lokutya, ukufuywa kwezilwanyana kunye nophando lwebhayoloji yeemolekyuli kunye nezinye iinkalo.
1. Indlela yokufunxa, ekwabizwa ngokuba yi-pipetting method, kukukhupha i-nucleic acid ngokufaka amaso emagnethi kunye nokudlulisa ulwelo. Ngokubanzi, ukudluliselwa kuphunyezwa ngokulawula ingalo yerobhothi ngokusebenzisa inkqubo yokusebenza. Inkqubo yokutsalwa imi ngolu hlobo lulandelayo:

I-1) I-Lysis: Yongeza isisombululo se-lysis kwisampuli, kwaye uqaphele ukuxuba kunye nokuphendula ngokupheleleyo kwesisombululo sokuphendula ngokunyakaza komatshini kunye nokufudumeza, iiseli zihlanjululwa, kwaye i-nucleic acid ikhutshwe.

I-2) I-Adsorption: Yongeza amaso amagnetic kwisampulu ye-lysate, xuba ngokucokisekileyo, kwaye usebenzise amaso amagnetic ukuze ube nobudlelwane obuqinileyo be-nucleic acids phantsi kwetyuwa ephezulu kunye ne-pH ephantsi ukuze ubhengeze i-nucleic acids. Ngaphantsi kwesenzo sentsimi yamagnetic yangaphandle, ubuhlalu bamagnetic buhlukaniswe kwisisombululo. , sebenzisa incam ukususa ulwelo kwaye ululahle kwitanki yenkunkuma, kwaye ulahle incam.

3) Ukuhlamba: Susa umhlaba wemagnethi wangaphandle, buyisela inqaku elitsha kwaye wongeze isithinteli sokuhlamba, xuba kakuhle ukususa ukungcola, kwaye ususe ulwelo phantsi kwesenzo semagnethi yangaphandle.

4) I-Elution: Susa umhlaba wangaphandle wemagnethi, buyisela inqaku elitsha, yongeza isithinteli se-elution, xuba kakuhle, kwaye emva koko wahlule i-nucleic acid ebotshiweyo kumaso azibuthe ukuze ufumane i-nucleic acid ecocekileyo.
2. Indlela yebha yemagnethi

Indlela ye-magnetic rod iqonda ukuhlukana kwe-nucleic acids ngokulungisa ulwelo kunye nokudlulisa amaso amagnetic. Umgaqo kunye nenkqubo ziyafana nezo zendlela yokufunxa, kodwa umahluko yindlela yokwahlula phakathi kwamaso kazibuthe kunye nolwelo. Indlela yemagnethi yebha kukwahlula amaso emagnethi kulwelo olulahlwayo ngokufaka intonga kazibuthe ukuya kumaso kazibuthe, kwaye uwabeke kulwelo olulandelayo ukuqonda ukutsalwa kwe-nucleic acid.