Ukwahlula kunye nokuhlanjululwa kweeprotheni zisetyenziswa ngokubanzi kuphando lwe-biochemistry kunye nokusetyenziswa kwaye sisakhono esibalulekileyo sokusebenza. I-SCG yeProtein Purification System Company-Saipu Instrument iye yaqulunqa ukuhlukana okungcolileyo kunye nomxholo wokwahlula okulungileyo.iprotheniukuhlanjululwa komntu wonke. Iseli eqhelekileyo ye-eukaryotic inokuqulatha amawaka eeprotheyini ezahlukeneyo, ezinye zizityebi kakhulu kwaye ezinye zineekopi ezimbalwa kuphela. Ukuze ufunde iprotheni ethile, kuyimfuneko ukucoca kuqala iprotheni evela kwezinye iiprotheni kunye neemolekyuli ezingenazo iiprotheni.

Ukwahlula okurhabaxa

Xa i-protein extract (ngamanye amaxesha ixutywe kunye ne-nucleic acids, i-polysaccharides, njl.)iprothenikobunye ukungcola. Ngokubanzi, eli nyathelo lokwahlula lisebenzisa iindlela ezinje ngetyuwa ngaphandle, ukuqokelelwa kwamanqaku e-isoelectric kunye nokuqhekeka kwe-solvent ye-organic. Ezi ndlela zibonakaliswe ngokulula kunye nomthamo omkhulu wokucubungula, onokususa ukungcola okuninzi kwaye ugxininise isisombululo seprotheni. Ezinye izicatshulwa zeprotheyini zinkulu ngomthamo kwaye azifanelekanga ukugxininiswa ngokuqokelela okanye ukutyumba ngaphandle. Unokukhetha i-ultrafiltration, i-gel filtration, i-vacuum epholileyo yokumisa okanye ezinye iindlela zokuxilisa.

Ukwahlula okulungileyo

Emva kokuqhekeka okukrakra kwesampulu, umthamo uncinci, kwaye ubuninzi bokungcola bususiwe. Ukucocwa ngakumbi, iindlela zechromatography ngokubanzi zibandakanya ukuhluzwa kwejeli, ukutshintshiselana nge-ion chromatography, i-adsorption chromatography, kunye ne-affinity chromatography. Ukuba kuyimfuneko, unokukhetha kwakhona i-electrophoresis, kubandakanywa i-electrophoresis yendawo, i-isoelectric point set, njl. njengenkqubo yokugqibela yokucoca. Indlela esetyenziswayo yokwahlula inqanaba lolwahlulo idla ngokuba yincinci kucwangciso, kodwa inesisombululo esiphezulu.

I-Crystallization yinkqubo yokugqibela yokuhlukana kweeprotheni kunye nokuhlanjululwa. Nangona inkqubo ye-crystallization ayiqinisekisi ukuba iprotheni kufuneka ibe yinto efanayo, kuphela xa iprotheni ethile inenzuzo kwisisombululo sokwenza i-crystal. Inkqubo ye-crystallization ngokwayo ihamba kunye neqondo elithile lokuhlanjululwa, kwaye i-recrystallization inokususa inani elincinci leprotheni egqwethiweyo. Ekubeni denaturediprotheniayizange ifunyanwe ngexesha lenkqubo ye-crystallization, i-protein crystallization ayilona nje uphawu lokucoceka, kodwa kunye nesikhokelo esinamandla sokugqiba ukuba imveliso ikwimeko yendalo.