------" I-Ultrafiltration & Microfiltration " Iinjongo ezininzi, ukuqonda ukutshintshwa kwasekhaya!
BM Life Science,Umbhobho we-Ultrafugal Centrifugal (Isihluzo se-Centrifugal)Series Products
Iqukethe ityhubhu yokucoca yangaphakathi (nge-membrane) + ityhubhu ye-centrifuge yangaphandle, enokuthi ihlambuluke ngokukhawuleza kwaye iphumelele kwaye igxininise ukuya kwi-15ml yeesampuli zezinto eziphilayo. Uyilo olulodwa oluthe nkqo kunye nommandla wokucoca ophakamileyo unokubonelela ngesampulu ekhawulezayo yokucubungula umthamo kunye nesantya esiphezulu sokubuyisela isampuli (ngokuqhelekileyo ≥90% ihlambulule isisombululo sokuqala), ngelixa igcina ukuhluzwa okuncinci kunye nokugxininiswa kwendawo yokugcina umsebenzi weprotheyini kunye neConformation.Uyilo olulodwa oluthe nkqo lunokwenza i-polarze i-solute kunye nokunciphisa kakhulu ukungcola kwe-membrane yokucoca okubangelwa yi-centrifugation elandelayo. Ngokulungisa indawo yokumisa isihluzo somzimba kwisixhobo sokucoca, inokuthintela isihluzo se-centrifugal ekomiseni isampuli okanye ibangele ilahleko yesampulu ngenxa yokujikeleza okugqithisileyo. . Iintlobo ngeentlobo ze-ultrafiltration molecular weight cutoff (MWCO) izihluzi ze-centrifugal zisebenzisa i-high-purity, i-adsorption ephantsi ye-PES kunye nee-membrane ze-RCE, ezineprotheyini ephantsi kakhulu kunye namazinga okubopha i-nucleic acid.
I-BM Life Science, imveliso yegumbi elicocekileyo kuzo zonke iikhonkco zeUmbhobho we-Ultrafugal Centrifugal (Isihluzo se-Centrifugal), ukusebenza komgca wendibano, ulawulo olupheleleyo lwe-ERP, iimveliso ze-ultra-pure, akukho DNase / RNase, akukho inhibitors ye-PCR, akukho mthombo wokushisa. I-BM Life Science, zonke iisayizi zeUmbhobho we-Ultrafugal Centrifugal (Isihluzo se-Centrifugal)iimveliso zothotho zenziwe ngokwezifiso, uthotho lweemveliso zizinzile kwiibhetshi ezinomahluko omncinci phakathi kweebhetshi kunye nomgangatho ophezulu. Zinokutshintshwa zimveliso zasekhaya kwaye zithunyelwe eJapan, eMzantsi Korea, eYurophu naseUnited States. Isetyenziswa ngokubanzi kwisampulu yokwahlulwa kunye novavanyo lokucoca oluqhelekileyo.
Isicelo
1 UkuhluzaUkutshintshwa kwe-buffer, ukucocwa, ukugxila, kunye nokukhupha ityuwa kwii-antigens, izilwa-buhlungu, ii-enzymes, kunye nezinye iiproteni, kunye neesampulu zebhayoloji ezifana ne-nucleic acids kunye ne-microorganisms, ukukhutshwa kwetyuwa kumalungu e-chromatographic, ukwahlukana kweemolekyuli zebhayoloji kwinkcubeko yezicubu okanye isisombululo se-cell lysis. ye-primers, izihlanganisi, okanye iziphawuli zemolekyuli ezivela kwimixube yokusabela, ukugxilwa kwentsholongwane okanye ukwahlula, kunye nokwahlula okukrwada kwemixube yebhayoloji.
2 Microfiltrationihlukanisa i-nucleic acids kunye neeprotheni kwiigels, iisampulu ziyahluzwa kwaye zicaciswe, kwaye amasuntswana acutshungulwayo okanye amaso aqokelelwa kwaye ahlanjwe.
Uphawu lweMveliso
★Umlinganiselo ophezulu woxinaniso, onokufikelela ngokulula kumlinganiselo we-80-100 woxinaniso;
★Isantya soxinzelelo lwesampulu sikhawuleza, kwaye ixesha lokugxininiswa ngokubanzi yimizuzu eyi-10-60. Ikwanayo noyilo lokutshixa oluchasene nokomisa ukuthintela umonakalo kwisampulu ngenxa ye-centrifugation egqithisileyo;
★ Izinga lokubuyisela isampuli liphezulu kwaye linokufikelela kwinqanaba lokubuyisela ngaphezu kwe-90%;
★ I-adsorption ephantsi, i-PES/RC inwebu kunye noyilo olugudileyo eludongeni luneprotein ephantsi kakhulu kunye nesantya sokubopha i-biomolecule;
4ml/15ml, 15ml/50ml
★ Iinkcukacha ezipheleleyo, iityhubhu ze-ultrafiltration centrifuge ezinemiqulu emibini ye-4ml/15ml kunye ne-15ml/50ml, ezinobukhulu obahlukeneyo bobupore, obufumaneka kwi-3KD, 5KD, 10KD, 30KD, 50KD kunye ne-100KD ukuhlangabezana neemfuno ezahlukeneyo zokunqunyulwa kwemolekyuli;
★ Ukusebenza ngokulula, yonke inkqubo yokwahlula kunye nokucoca ingagqitywa ngeI-Multifunctional Centrifuge, kulula ukusebenza;
★ Imveliso ihlambulukile, zonke izixhumanisi ziveliswa kwigumbi elicocekileyo, ukusebenza komgca wendibano, ulawulo olupheleleyo lwe-ERP, imveliso i-ultra-pure, akukho DNase / RNase, akukho inhibitor ye-PCR, akukho mthombo wokushisa;
★ Inkcazo ephezulu, akukho mva kunye ne-auto-fluorescence, kwaye ayichaphazeli iziphumo zobhaqo;
★ Ukusebenza okuthembekileyo, ukutywinwa okulungileyo, kwaye akusebenzi kunye neesampuli zovavanyo, iziphumo zovavanyo zithembekile, kwaye zinokumelana nokushisa okuphezulu kwe-100 ℃!
★ Inoluhlu olubanzi lwezicelo. Kwizicelo ezahlukeneyo, iindidi ezimbini zeUmbhobho we-Ultrafugal Centrifugal (Isihluzo se-Centrifugal)iimveliso zechungechunge, i-4ml / 15ml kunye ne-15ml / 50ml zingeniswa ukuhlangabezana neemfuno ezahlukeneyo zabathengi, ukwamkela ukulungiswa kunye nophuhliso olusebenzayo!
Ulwazi lweodolo
| Ikati.No | Igama | Inkcazo | Inkcazo | IiPC/pk |
| UCT015RCE004003 | Umbhobho we-Ultrafugal we-Ultrafugal | 4/15ml, 3kD | , iiMembranes zeRCE | 15 iipcs / isikhwama |
| UCT015RCE004010 | Umbhobho we-Ultrafugal we-Ultrafugal | 4/15ml, 10kD | IiMbrane zeRCE | 15 iipcs / isikhwama |
| UCT015RCE004030 | Umbhobho we-Ultrafugal we-Ultrafugal | 4/15ml, 30kD | IiMbrane zeRCE | 15 iipcs / isikhwama |
| UCT015RCE004100 | Umbhobho we-Ultrafugal we-Ultrafugal | 4/15ml, 100kD | IiMbrane zeRCE | 15 iipcs / isikhwama |
| UCT050RCE015003 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 3kD | IiMbrane zeRCE | 24 iipcs / isikhwama |
| UCT050RCE015010 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 10kD | IiMbrane zeRCE | 24 iipcs / isikhwama |
| UCT050RCE015030 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 30kD | IiMbrane zeRCE | 24 iipcs / isikhwama |
| UCT050RCE015100 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 100kD | IiMbrane zeRCE | 24 iipcs / isikhwama |
| UCT050PES015005 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 5kD | PES Membranes | 12 iipcs / isikhwama |
| UCT050PES015010 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 10kD | PES Membranes | 12 iipcs / isikhwama |
| UCT050PES015030 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 30kD | PES Membranes | 12 iipcs / isikhwama |
| UCT050PES015050 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 50kD | PES Membranes | 12 iipcs / isikhwama |
| UCT050PES015100 | Umbhobho we-Ultrafugal we-Ultrafugal | 15/50ml, 100kD | PES Membranes | 12 iipcs / isikhwama |
| CF050PES015C010 | Isihluzo se-Centrifugal | 15/50ml, 0.1um | 0.1um, iiMembranes zePES | 12 iipcs / isikhwama |
| CF050PES015C020 | Isihluzo se-Centrifugal | 15/50ml, 0.2um | 0.2um, iiMembranes zePES | 12 iipcs / isikhwama |
| CF050PES015C045 | Isihluzo se-Centrifugal | 15/50ml, 0.45um | 0.45um, iiMembranes zePES | 12 iipcs / isikhwama |
| UCT** | Umbhobho we-Ultrafugal we-Ultrafugal | Ukwenza ngokwezifiso | Ukwenza ngokwezifiso | ** iipcs / ibhegi |
| CF** | Isihluzo se-Centrifugal | Ukwenza ngokwezifiso | Ukwenza ngokwezifiso | ** iipcs / ibhegi |
BM Life Sciences, Ultrafiltration Centrifugal Tube (Centrifugal Filter) series parameters product and experimental data
I-Ultrafiltration Centrifugal Tube (Isihluzo se-Centrifugal) uthotho lwemveliso yokusebenza kunye neenkcukacha zovavanyo
I-Ultrafiltration Centrifugal yeesampulu:
5 KD 0.25mg/ml Cyt C;
10 KD1mg/ml OVA;
30/50 KD 1mg/ml BSA;
100 KD 1mg/ml IgG.
Iimeko zovavanyo : i-rotor ethe tye, i-centrifugal force 4000×g, 8℃, ukuqala kwe-15ml.
I-Ultrafiltration Centrifugal yeesampulu:
5 KD 0.25mg/ml Cyt C;
10 KD 1mg/ml OVA;
30/50 KD 1mg/ml BSA;
100 KD 1mg/ml IgG.
Iimeko zovavanyo: i-rotor ye-angle esisigxina, i-centrifugal force 5000×g, 8℃, ukuqala kwe-12ml.
Amazinga aqhelekileyo okwaliwa kwemigangatho yobunzima bemolekyuli yeprotheyini (4000g x 15ml isiqalo somthamo werotor eshukumayo okanye 5000g x 12ml ukuqalisa umthamo we-engile yerotha esisigxina)
| imigangatho yobunzima bemolekyuli/noxinaniso | ubunzima bemolekyuli | Ityhubhu yokucoca i-NMWL | amazinga okulahlwa % Seta ibhakethi | Amazinga okwala % i-engile esisigxina | ixesha le-centrifugation |
| B-12 ivithamin | 1350 | 3K | ＜25 | ＜25 | 60 |
| (0.25mg/ml) iCytochrome C | 12400 | ＞95 | ＞95 | 60 | |
| (1mg/ml) α-Chymotrypsinogen | 25000 | ＞95 | ＞95 | 60 | |
| B-12 (0.2mg/ml) ivithamin | 1350 | 10K | ＜5 | ＜5 | 30 |
| 0.25mg/ml）Cytochrome C | 12400 | ＞95 | ＞95 | 30 | |
| (1mg/ml) α-Chymotrypsinogen | 25000 | ＞95 | ＞95 | 30 | |
| ivithamin B-12 (0.2mg/ml) | 1350 | 30K | ＜5 | ＜5 | 20 |
| Cytochrome C 0.25mg/ml） | 12400 | ＜10 | ＜10 | 20 | |
| Ovalbumin (1mg/ml) | 45000 | ＞95 | ＞95 | 20 | |
| BSA (1mg/ml) | 67000 | ＞95 | ＞95 | 20 | |
| Cytochrome C (0.25mg/ml) | 12400 | 50K | ＜5 | ＜5 | 10 |
| Ovalbumin (1mg/ml) | 45000 | 65 | 55 | 10 | |
| BSA (1mg/ml) | 67000 | ＞90 | ＞90 | 10 | |
| I-Ovalbumin (1mg/ml | 45000 | 100K | ＜25 | ＜15 | 20 |
| IgG (1mg/ml) | 156000 | ＞90 | ＞90 | 20 | |
| Thyroglobulin (0.5mg/ml) | 677000 | ＞90 | ＞90 | 20 |
Izinga lokubuyisela
Umthamo wokugcinwa kwe-15ml ultrafiltration centrifuge tube membrane ichazwa yi-molecular weight cutoff (MWCO).
Ubunzima bemolekyuli eSolute kufutshane ne-MWCO bunokugcinwa kuphela ngokuyinxenye. Ukuze ufumane ezona mpawu zilungileyo zokugcina,
Nceda usebenzise inwebu yokucoca i-MWCO enobunzima bemolekyuli ye-1/3 okanye ngaphantsi kwe-solute.
Ukulinganisa izinga lokubuyisela imveliso
Ukuthelekiswa kwamazinga okubuyisela iiprotheyini kunye nomthamo ofileyo weempawu ezahlukeneyo zeetyhubhu ze-ultrafiltration
| I-10KD Ukuthelekiswa kwamazinga okubuyisela iprotheni kunye nokufa imiqulu yeempawu ezahlukeneyo zeetyhubhu ze-ultrafiltration | |||
| Uphawu | (%) Izinga loBuyiselo | (μl) Umthamo ofileyo | |
| BM | 1㎎/ml Uxinzelelo lweprotheyini | 90 | 87 (Uxinzelelo lweprotheyini1㎎/ml) |
| 0.1㎎/ml Uxinzelelo lweprotheyini | 83 | ||
| Ibhrendi M | 1㎎/ml Uxinzelelo lweprotheyini | 93 | 47 (Uxinzelelo lweprotheyini1㎎/ml) |
| 0.1㎎/ml Uxinzelelo lweprotheyini | 88 | ||
| Uphawu S | 1㎎/ml Uxinzelelo lweprotheyini | 91 | 70 (Uxinzelelo lweprotheyini1㎎/ml) |
| 0.1㎎/ml Uxinzelelo lweprotheyini | 85 | ||
| Brand K | 1㎎/ml Uxinzelelo lweprotheyini | 89 | 186 (Uxinzelelo lweprotheyini1㎎/ml) |
| 0.1㎎/ml Uxinzelelo lweprotheyini | 77 | ||
| Brand J | 1㎎/ml Uxinzelelo lweprotheyini | 83 | |
| 0.1㎎/ml Uxinzelelo lweprotheyini | 50 | ||
| Iimeko zovavanyo: Iprotheyini ye-OVA (ubunzima bemolekyuli 45KD), i-rotor ethe tye 4000g | |||
Inkcazo yokukhetha ireferensi yeetyhubhu ze-ultrafiltration ezinobunzima beemolekyuli ezahlukeneyo
| ivolumu yokuqala | ＜0.5ml | ＜2ml | ＜4ml | ＜15ml | |
| Iprotheni | |||||
| (MW) ubunzima bemolekyuli | MWCO (Da) ukusika ubunzima bemolekyuli | ||||
| 6K＜MW＜20K | 3000 | 3000 | 3000 | 3000 | |
| 20K＜MW＜60K | 10000 | 10000 | 10000 | 10000 | |
| 60K＜MW＜100K | 30000 | 30000 | 30000 | 30000 | |
| 100K＜MW＜200K | 50000 | 50000 | 50000 | 50000 | |
| 200K＜MW | 100000 | 100000 | 100000 | 100000 | |
| I-nucleic acids enemisonto enye kunye ne-double-stranded | |||||
| ubude | MWCO (Da) ukusika ubunzima bemolekyuli | ||||
| 137-1159bp | 3000 | 3000 | 3000 | 3000 | |
| nanoparticles | |||||
| (DIA) ubungakanani bamasuntswana | MWCO (Da) ukusika ubunzima bemolekyuli | ||||
| 1.5＜DIA＜3nm | 3000 | 3000 | 3000 | 3000 | |
| 3＜DIA＜5nm | 10000 | 10000 | 10000 | 10000 | |
| 5＜DIA＜7nm | 30000 | 30000 | 30000 | 30000 | |
| 7＜DIA＜10nm | 50000 | 50000 | 50000 | 50000 | |
| 10 nm＜DIA | 100000 | 100000 | 100000 | 100000 | |
Ixesha lokuposa: Feb-18-2024
