I-Solid phase extraction (SPE) yinkqubo yokukhutshwa komzimba equka izigaba zolwelo kunye neziqinileyo. Kwinkqubo yokukhutshwa, amandla e-adsorption ye-slid ukuya ku-analyte inkulu kune-sample mother yotywala. Xa isampuli idlula kwiSPEikholamu, i-analyte i-adsorbed kumphezulu oqinileyo, kunye namanye amacandelo adlula kwikholamu kunye notywala besampula. Ekugqibeleni, i-analyte isuswe nge-solvent efanelekileyo Eluted. I-SPE inoluhlu olubanzi lwezicelo, ezifana nohlalutyo lwe-biological fluids kuquka igazi, umchamo, i-serum, i-plasma kunye ne-cytoplasm; uhlalutyo lokwenziwa kobisi, iwayini, iziselo kunye nejusi yeziqhamo; ukuhlalutya kunye nokubeka iliso kwimithombo yamanzi; iziqhamo, imifuno, iinkozo, kunye neentlobo ezahlukeneyo zezicubu zezityalo Izicubu zezilwanyana; amayeza aqinileyo afana neepilisi. Uhlalutyo lwe-pesticide kunye ne-herbicide residues kwiziqhamo, imifuno kunye nokutya, uhlalutyo lwe-antibiotics kunye neziyobisi zeklinikhi, njl.

(1) Thatha ngokucokisekileyo isixhobo sokutsalwa kwesigaba esiqinileyo kwaye usibeke ngobunono kwibhentshi yokusebenza.

(2) Ngononophelo khupha isigqubuthelo esingaphezulu seSPEisixhobo (ubambe ngobunono ukuze ungonakalisi ityhubhu encinci), faka ityhubhu yovavanyo oluqhelekileyo kumngxuma wesahlulelo kwigumbi lokucoca, kwaye emva koko uvale ikhava eyomileyo ephezulu, kwaye uqinisekise ukuba isigqubuthelo sikhokelwa phantsi. Umbhobho wokuhamba kunye netyhubhu yokuvavanya ihambelana nganye nganye, kwaye ikhonkco lokutywinwa kwesikwele seplate yokugubungela inomsebenzi wokutywina kakuhle kunye negumbi lokucoca. Ukuba akulula ukutywinwa, inokuqiniswa ngebhendi yerabha ukunyusa ukuqina.

(3) Ukuba uthenge ukulungiswa okuzimeleyo, kufuneka uqale ufake i-valve yokulungelelanisa kwingxuma yokukhutshwa kwekhava;

(4) Ukuba akukho mfuneko yokuba wenze iisampulu ezili-12 okanye ezingama-24 ngexesha, qhaga ityhubhu yevalve yenaliti kumngxunya ongasetyenziswanga;

(5) Ukuba isivalo solawulo esizimeleyo sithengiwe, jika iqhoshana levalve yolawulo lomngxuma ongasetyenziswanga wokutsalwa ukuya kwindawo yokutywina ethe tye;

(6) Faka ikhatriji yesigaba esiluqilima kumngxuma wokutsalwa okanye umngxuma wevalvu yesigqubuthelo esingaphezulu (jika iqhosha levalve elawulayo kwindawo evuleke nkqo); qhagamshela isixhobo sokukhupha kunye nepompo yokucoca nge-hose, kwaye uqinise i-valve yokulawula uxinzelelo;

(7) Faka iisampulu okanye i-reagents ukuba ikhutshwe kwikholamu yokukhutshwa, kwaye iqalise ipompo ye-vacuum, ngoko isampuli kwikholamu yokukhupha iya kuhamba ngekholomu yokukhutshwa kwityhubhu yokuvavanya engezantsi phantsi kwesenzo soxinzelelo olubi. Ngeli xesha, isantya sokuhamba kwamanzi sinokuhlengahlengiswa kwaye silawulwe ngokulungelelanisa i-valve yokunciphisa uxinzelelo.

(8) Emva kokuba ulwelo olukwityhubhu yenaliti lumpontshiwe, cima impompo yokufunxa, khupha ikholamu yokuphucula kwisixhobo, susa isiciko esingaphezulu sesixhobo, khupha ityhubhu yovavanyo kwaye uyigalele.

(9) Ukuba awufuni ukusebenzisa ityhubhu yokuvavanya ukudibanisa ulwelo, unokukhupha i-tube rack yokuvavanya, uyibeke kwisitya sobukhulu obufanelekileyo, kwaye uyikhuphe emva kokutsalwa kokuqala.

(10) Faka ityhubhu yovavanyo ecocekileyo kwisixhobo, vala isigqubuthelo, faka i-cartridge ye-SPE, yongeza i-solvent efunekayo kwityhubhu yenaliti, qalisa impompo yokufunxa, cima umbane emva kokuba ulwelo lukhutshiwe, kwaye ukhuphe umbane. ityhubhu yovavanyo yokusetyenziswa. Utsalo kunye nolungiselelo lwesampulu lugqityiwe.

(11) Beka ityhubhu yokuvavanya kwisixhobo sokumisa initrogen kwaye uhlambulule kwaye ugxininise ngenitrogen, kwaye ukulungiswa kugqityiwe.

(12) Lahla i-solvent kwi-tube yokuvavanya, kwaye uhlambulule ityhubhu yokuvavanya ukuze uyisebenzise kwakhona.

(13) Ukuze kugcinwe iindleko zokusebenzisa iSPEikholamu, emva kokusetyenziswa ngakunye, ikholamu ye-SPE kufuneka ihlanjwe nge-eluent ukuqinisekisa iimpawu zokupakishwa kwayo.