Ibhotile yesampuli isitya sokuhlalutya isixhobo sezinto eziza kuhlalutywa, kwaye ukucoceka kwayo kuchaphazela ngqo umphumo wohlalutyo. Eli nqaku lishwankathela iindlela ezahlukeneyo zokucoca ibhotile yesampulu yechromatographic, kwaye lijonge ukubonelela ngereferensi enentsingiselo kuye wonke umntu. Ezi ndlela ziqinisekisiwe ngabahlobo kunye nabangaphambili. Zinempembelelo entle yokuhlamba kwiintsalela ezinyibilikayo ezinamafutha kunye neentsalela ze-organic reagentibhotile yesampuli yechromatography. Ucoceko luhlangabezana neemfuno, amanyathelo okucoca alula, kwaye ixesha lokucoca liyancipha, kwaye inkqubo yokucoca ihambelana nokusingqongileyo .

Nceda wenze ukhetho lwakho ngokusekelwe kwimeko yakho yaselabhoratri!

Okwangoku, ngomdla okhulayo kumgangatho wokutya kunye nokhuseleko kuzo zonke iinkalo zobomi, itekhnoloji yohlalutyo lwechromatographic isanda kusetyenziswa kumgangatho wokutya kunye novavanyo lokhuseleko, ngakumbi kwicandelo lovavanyo lwemveliso yezolimo, itekhnoloji yohlalutyo lwechromatographic isetyenziswe ngokubanzi. Ezweni lam, inani elikhulu leemveliso zezolimo (ezinye iimveliso zeekhemikhali, i-organic acids, njl.) kufuneka zivavanywe nge-chromatography yamanzi kunye ne-chromatography yegesi minyaka yonke. Ngenxa yenani elikhulu leisampulu, kukho inani elikhulu leebhotile zesampulu ezifuna ukucocwa ngexesha lenkqubo yokubhaqwa, engachithi nje ixesha kwaye inciphise ukusebenza kakuhle, kodwa ngamanye amaxesha ibangela ukutenxa kwiziphumo zovavanyo ngenxa yokucoceka kombane. iibhotile zesampuli ezicocekileyo.

Iibhotile yesampuli yechromatographicubukhulu becala yenziwe ngeglasi, ayifane ibe yiplastiki. Iibhotile zesampulu ezilahlwayo zineendleko, ziyinkcitho, kwaye zibangela ungcoliseko olumandla lokusingqongileyo. Iilabhoratri ezininzi zicoca iibhotile zesampuli kwaye ziphinde zisetyenziswe. Okwangoku, iindlela eziqhele ukusetyenziswa kwiilabhoratri zokucoca ibhotile yesampuli ikakhulu ukongeza umgubo wokuvasa, isefu, isinyibilikisi se-organic, kunye ne-acid-base lotion, kwaye emva koko ukhuhle ngetyhubhu encinci yovavanyo. Le ndlela yesiqhelo yokukhuhla ineziphene ezininzi. Isebenzisa isixa esikhulu sesicoci kunye namanzi, ithatha ixesha elide ukuyihlamba, kwaye ithanda ukushiya iikona ezifileyo. Ukuba yisampuli yebhotile yeplastiki, kulula ukushiya amanqaku ebrashi kudonga lwebhotile yangaphakathi, nto leyo ethatha abantu abaninzi. Kwi-glassware engcoliswe kakhulu yi-lipid kunye ne-protein residues, isisombululo se-alkaline lysis sisetyenziselwa ukucoca, kwaye iziphumo ezilungileyo zifezekiswa.

Xa uhlalutya iisampuli, ukucocwa kwebhotile yokutofa kubaluleke kakhulu. Ngokwendlela yokuhlamba i-glassware, indlela yokucoca ikhethwe ngokwezinga lokungcola, kwaye akukho ndlela emiselweyo. Indlela isishwankathelo:

1. Thela isisombululo sovavanyo kwibhotile eyomileyo

2. Gxila yonke into kwi-95% yotywala, uhlambe kabini nge-ultrasonic kwaye uyithulule, kuba utywala bungena lula kwi-vial ye-1.5mL kwaye inokuxutywa kunye neninzi ye-solvents ye-organic ukuze ufezekise umphumo wokucoca.

3. Thela emanzini acocekileyo, kwaye uhlambe i-ultrasonically kabini.

4. Galela i-lotion kwibhotile eyomileyo kwaye ubhake kwi-110 degrees Celsius nge-1 ~ 2 iiyure. Ungaze ubhake kwiqondo lokushisa eliphezulu.

5. Pholisa kwaye ugcine.