Inkqubo jikelele yokutsalwa kwesigaba esiqinileyo imi ngolu hlobo lulandelayo:

1. Ukuvuselela i-adsorbent: Hlanza i-cartridge yesigaba esiqinileyo kunye ne-solvent efanelekileyo ngaphambi kokuba ukhuphe isampuli ukugcina i-adsorbent emanzi, enokuthi i-adsorb i-compounds ejoliswe kuyo okanye i-interfering compounds. Iindlela ezahlukeneyo zokuvula i-cartridge yesigaba esiqinileyo zisebenzisa izinyibilikisi ezahlukeneyo:

(1) I-polar ebuthathaka okanye i-non-polar adsorbents esetyenziswa kwi-reverse-phase solid-phase extraction ngokuqhelekileyo ihlanjululwe nge-solvent enyibilikayo yamanzi, njenge-methanol, kwaye ihlanjululwe ngamanzi okanye isisombululo se-buffer. Kwakhona kunokwenzeka ukuba uhlambulule nge-solvent enamandla (efana ne-hexane) ngaphambi kokuba uhlanjululwe nge-methanol ukuphelisa ukungcola okufakwe kwi-adsorbent kunye nokuphazamiseka kwabo kwikhompawundi ekujoliswe kuyo.

(2) I-adsorbent ye-polar esetyenziswe kwisigaba esiqhelekileyo sokutsalwa kwesigaba esiqinileyo sivame ukuchithwa kunye ne-solvent ye-organic (isampuli ye-matrix) apho ikhompawundi ekujoliswe kuyo ikhona.

3 xa isetyenziselwa iisampulu kwi-solvents ye-polar, inokuhlanjwa ngamanzi a-soluble organic solvents Emva kokuhlamba, hlambulula ngesisombululo samanzi esinexabiso elifanelekileyo le-pH kwaye iqulethe i-solvents kunye neetyuwa ezithile.

Ukuze ugcine i-sorbent kwi-cartridge ye-SPE imanzi emva kokusebenza kunye nangaphambi kokudibanisa isampuli, malunga ne-1 ml ye-solvent ukuze isebenze kufuneka igcinwe kwi-sorbent emva kokusebenza.

2. Ukulayisha isampulu: Galela ulwelo okanye isampulu ezinyibilikayo eqinileyo kwikhatriji esebenzayo isigaba esomeleleyo, uze usebenzise vacuum, uxinzelelo okanye centrifugation ukwenza isampuli ukungena adsorbent.

3. Ukuhlamba kunye ne-elution: Emva kokuba isampuli ingene kwi-adsorbent kunye nekhompawundi ekujoliswe kuyo i-adsorbed, i-compound egciniweyo ephazamisayo engenamandla inokuhlanjwa kunye ne-solvent ebuthathaka, kwaye ke ikhompawundi ekujoliswe kuyo inokukhutshwa kunye ne-solvent eyomeleleyo kwaye iqokelelwe. . I-Rinse kunye ne-Elution Njengoko kuchaziwe ngaphambili, i-eluent okanye i-eluent inokugqithiswa kwi-adsorbent ngokusebenzisa i-vacuum, uxinzelelo okanye i-centrifugation.

Ukuba i-adsorbent ikhethiweyo ukuba ibe buthathaka okanye ingabikho i-adsorption kwi-target target kunye ne-adsorption enamandla kwi-compound ephazamisayo, i-compound target ingaphinda ihlanjululwe kwaye iqokelelwe kuqala, ngelixa i-compound ephazamisayo igcinwa (adsorption). ) kwi-adsorbent, ezi zimbini ziyahlukana. Kwiimeko ezininzi, i-compound ekujoliswe kuyo igcinwa kwi-adsorbent, kwaye ekugqibeleni ihlanjululwe nge-solvent eqinile, eyona nto ihambelana nokuhlanjululwa kwesampuli.