Uvavanyo lwe-Nucleic acid kukufumanisa ukuba ngaba kukho i-nucleic acid (RNA) ye-coronavirus entsha emzimbeni wesifundo sovavanyo. I-asidi ye-nucleic yentsholongwane nganye iqulethe i-ribonucleotides, kwaye inani kunye nomyalelo we-ribonucleotides eziqulethwe kwiintsholongwane ezahlukeneyo zahlukile, okwenza intsholongwane nganye ikhethekileyo.
I-nucleic acid ye-coronavirus entsha ikwahlukile, kwaye ubhaqo lwe-nucleic acid luphawu oluthile lwe-nucleic acid ye-coronavirus entsha. Ngaphambi kovavanyo lwe-nucleic acid, kuyimfuneko ukuqokelela iisampulu zesikhohlela somxholo, i-swab yomqala, i-bronchoalveolar lavage fluid, igazi, njl. Ukufunyaniswa kwe-nucleic acid entsha ye-coronavirus ngokuqhelekileyo kusetyenziselwa ukubonwa kwesampulu yomqala. Isampulu yahlulwe kwaye yahlanjululwa, kwaye i-coronavirus entsha ye-nucleic acid ikhutshwa kuyo, kwaye amalungiselelo ovavanyo alungile.

Ubhaqo lwe-nucleic acid entsha ye-coronavirus ikakhulu isebenzisa itekhnoloji yobungakanani be-fluorescence ye-RT-PCR, eyindibaniselwano yetekhnoloji yobungakanani be-fluorescence ye-PCR kunye netekhnoloji ye-RT-PCR. Kwinkqubo yokufumanisa, iteknoloji ye-RT-PCR isetyenziselwa ukuguqula i-nucleic acid (RNA) ye-coronavirus entsha kwi-deoxyribonucleic acid (DNA); emva koko iteknoloji ye-fluorescence quantitative PCR isetyenziselwa ukuphindaphinda i-DNA efunyenwe ngobuninzi. I-DNA ephindaphindwayo ichongiwe kwaye ibhalwe ngeprobe yesondo. Ukuba kukho i-nucleic acid entsha ye-coronavirus, isixhobo sinokubona isiginali ye-fluorescent, kwaye, njengoko i-DNA iqhubeka nokuphindaphinda, isiginali ye-fluorescent iyaqhubeka nokukhula, ngaloo ndlela ibona ubukho be-coronavirus entsha.