Uhlalutyo lweBlot kwiBiopharmaceutical, Medical and Other Fields
"Isicwangciso se-14 seminyaka emihlanu" iSicwangciso soPhuhliso lwe-Bioeconomy siphakamisa ukuba uqoqosho lwendalo kufuneka luqhutywe kuphuhliso kunye nenkqubela phambili yesayensi yobomi kunye ne-biotechnology, ngokusekelwe kukhuseleko, uphuhliso kunye nokusetyenziswa kwemithombo yebhayoloji, kunye nokusekwe kuhlanganiso olubanzi nolunzulu lwendalo. amayeza, impilo, ezolimo, amahlathi, namandla. , ukhuseleko lokusingqongileyo, imathiriyeli kunye namanye amashishini; kucacile ukuba uphuhliso lwe-bio-economy lukhokelo olubalulekileyo lokuthobela intsingiselo yendaleko ekhawulezileyo ye-biotechnology yehlabathi kunye nokufikelela kwinqanaba eliphezulu lokuzimela kwezenzululwazi kunye nobuchwepheshe. Linyathelo elibalulekileyo ukulima kunye nokwandisa i-bio-industry kunye nokukhuthaza uphuhliso loqoqosho olukumgangatho ophezulu. Ukuhlangabezana nokukhula okukhawulezayo kweemfuno zobomi kunye nempilo kunye nokwanelisa ulangazelelo lwabantu lobomi obungcono sisiqinisekiso esibalulekileyo sokuqinisa uthintelo nolawulo lwe-biosecurity ye-biosecurity kunye nokukhuthaza ukuphuculwa kwenkqubo yolawulo yesizwe kunye namandla olawulo.
Ekuphenduleni umnxeba wesizwe, i-BM izinikele ekoyiseni iteknoloji yokuvelisa ifilimu ephezulu kunye nokuqonda ngokuthe ngcembe ukutshintshwa kwezinto ezithengiswayo zexabiso eliphezulu kwintsimi yesayensi yobomi. NgoMeyi ka-2023, ukuveliswa kobuninzi be-immunochromatography ye-NC membranes kwaphunyezwa ngempumelelo kwaye kwasetyenziswa kwii-reagents ezahlukeneyo zokufumanisa ngokukhawuleza. Okwangoku, ifilimu ye-NC isetyenzisiwe kuxilongo lwe-in vitro yasekhaya, ukhuseleko lokutya, uvavanyo olukhawulezayo lweziyobisi kunye nezinye iinkalo, kwaye iphumelele ukuthunyelwa ngaphandle kunye nokukhuphisana nezigebenga zamazwe ngamazwe kwimarike! Emva kokugqiba intetho yentengiso yefilimu ye-NC, emva kweenyanga ezininzi zophando lobugcisa liqela lethu lobugcisa, ekuphenduleni iimfuno ezingxamisekileyo zabasebenzisi kwintsimi yesayensi yobomi behlabathi ukunciphisa ixabiso lezinto ezityiwayo zexabiso eliphezulu, siqalise ngempumelelo iimbumba zokuvala. , efanelekileyo kwi-biopharmaceuticals, iyeza kunye nezinye iindawo. Uhlalutyo lweblothi yaseNtshona (Ukubhula eNtshona, iWB)
Intshayelelo kwiimpawu zeBM Blotting Membranes, : Ubungakanani bepore kunye nohlobo lweprotheyini esebenzayo 0.1μm ilungele iiproteni ezinobunzima bemolekyuli obungaphantsi kwe-7kDa 0.22μm ezifanelekileyo kwiiproteni ezinobunzima bemolekyuli obungaphantsi kwe-20kDa 0.45μm ezifanelekileyo kwiiproteni ezinobunzima bemolekyuli obukhulu kune-20kDa Main Imigaqo yokubophelela iiproteni Umbane ongatshintshiyo kunye ne-hydrophobicity Iimeko zogqithiso olusebenzayo kunye neendlela zokubona I-Chemiluminescence Ubhaqo lwe-Fluorescence olubhalwe nge-radiolabhele probe ukudaya ngokuthe ngqo I-antibody edityaniswe yi-enzyme Inzuzo:
1.Imvelaphi ephantsi, uvakalelo oluphezulu
2.Akukho mfuneko ye-reagent yotywala kwangaphambili
I-3.Isakhiwo esiyingqayizivele somgangatho kunye neempawu zenza i-signal-to-noise ratio ebalaseleyo Izinto eziphathekayo zivela kwimicu yendalo, ihambelana nokusingqongileyo, kwaye inokugcina iprotheni eboshiweyo ixesha elide.
Intshayelelo yetekhnoloji yohlalutyo lwe-WB yetekhnoloji yohlalutyo lwe-WB bubuchwepheshe obusetyenziswa kakhulu kwiinkalo zebhayoloji yemolekyuli, i-biochemistry, i-immunology kunye nezinye iinkalo. Le teknoloji isebenzisa ukubophelela okukhethekileyo kwee-antibodies kwiiprotheni ezithile kwizicubu okanye iisampulu zeseli ukufezekisa ukuchongwa kweprotheyini kunye nohlalutyo lwentetho esekelwe kwisikhundla kunye nobukhulu bebhanti yombala, oko kukuthi, uhlalutyo lomgangatho kunye nesiqingatha sobuninzi. Yacetywa okokuqala nguHarry Towbin weFriedrich Miescher Institute eSwitzerland ngo-1979. Sekuyiminyaka engaphezu kwama-40 edlulileyo kwaye ibe yindlela yophando lweprotheyini eqhelekileyo kakhulu kunye nesebenzayo.
Ixesha lokuposa: Feb-02-2024
