Isishwankathelo:

I-C18W (engavaliweyo) yikholomu yokukhutshwa kwe-PHASE C18 engatywinwanga esekelwe kwi-silica gel, kunye namaqela amaninzi asebenzayo e-silanol ebusweni anikezela ukusebenzisana okongeziweyo kwe-polar. Ukukhutshwa kweekhompawundi ze-nonpolar nge-hydrophobicity. Ngexesha elifanayo, umsebenzi weqela le-silanol wenza kube ngcono kune-C18 ukugcina ezinye i-metabolites zamachiza kunye neekhompawundi ezisisiseko, ngoko ke indlela yokugcinwa kwayo ibandakanya imiphumo ephakathi ye-non-polar kunye ne-polar yesibini. Ikholomu igcinelwe uninzi lwezinto eziphilayo kwaye inoluhlu olubanzi lokukhetha. Sisigaba sendalo yonke sokumisa sokutsalwa kwekhompawundi zepolar nezingezizo ezomhlaba.
Ikholamu ilingana ne-Aglient Accu Bond C18, Bond Elute C18 OH.

iinkcukacha

I-matrix: ijeli ye-silica
Iqela elisebenzayo: i-octadecyl engatywinwanga, iqela elisebenzayo le-silanol
Indlela yokwenza: ukutsalwa kwesigaba sokubuyisela umva
Umxholo weCarbon: 18%
Ubungakanani: 40-75 microns
Indawo engaphezulu: 300m2/g
Umyinge wokuvula: 60
Isicelo: umhlaba; Amanzi; Ulwelo lomzimba (i-plasma / umchamo, njl.); Ukutya; iyeza
Ukusetyenziswa okuqhelekileyo: ukwahlukana kwe-lipids, ukuhlukana kwe-ganglioside yeJPMHW kunye ne-United States CDFA indlela esemthethweni: izitshabalalisi kwiimveliso zendalo zokutya.
Indlela ye-AOAC: uhlalutyo lwe-pigment kunye neswekile ekutyeni, iziyobisi kunye ne-metabolite egazini, i-plasma kunye nomchamo, iprotheni, i-DNA macromolecule isampuli ye-desalting, ukutyetyiswa kwezinto eziphilayo kwisampuli yamanzi okusingqongileyo, ukutsalwa kwe-asidi ephilayo kwisiselo, ngolu hlobo lulandelayo: Ukukhutshwa kunye nokucocwa kwamayeza okubulala iintsholongwane. , barbiturates, phthalazines, caffeine, iziyobisi, idayi, ioyile enevumba elimnandi, iivithamin ezinyibilikayo ngamafutha, imichiza yokungunda, ukususa ukhula ii-agent, i-pesticides, i-carbohydrates, i-p-hydroxytoluene ester, i-phenol, i-phthalate esters, i-steroids, i-surfactants, i-theophylline, njl.