QuEChERS (Ho Potlaka, ho Bonolo, ha e theko e tlase, e sebetsa hantle, e Rugged ebile e Sireletsehile) lilemong tsa morao tjena e hlahisitse mekhoa ea morao-rao ea ho lokisa sampole bakeng sa ho lemoha lihlahisoa tsa temo. E ile ea qalisoa ka lekhetlo la pele ke M Anastassisdes oa lefapha la temo la United States (2003), 'me ka mor'a ho netefatsa le ho ntlafatsa haholo, mokhoa oa QuEChERS o ile oa behoa pele ka molao. Mekhoa ea ho lokisa ea QuEChERS ea masala a chefo e bolaeang likokoanyana ea SPE e ntlafatsa mofuta, e tšoana le phello ea tlhoekiso ea SPE, empa mohato oa ts'ebetso o khuts'oane haholoanyane, o na le litšobotsi tsa ho boloka nako, ts'ebetso e phahameng, moruo mme butle-butle e amoheloa ke tlhahlobo e akaretsang ea basebetsi. Ho sebelisa QuEChERS kit, ho hlokahala mehato e seng mekae feela ho phethela mohlala oa boitokiso ba tlhahlobo ea chefo e bolaeang likokoanyana ea mefuta e mengata le masalla a mangata.

Sehlahisoa sa BM - Q ke mohato o motle ka ho fetisisa oa Shenzhen BM Life Science o ipapisitse le nts'etsopele ea lihlahisoa tsa ts'ebetso tse potlakileng tsa QuEChERS, letoto la sampole e ka sebelisoa ho bona masala a chefo e bolaeang likokoanyana, ho lemoha masala a lithethefatsi tsa bongaka ba liphoofolo, li-additives tsa lijo, ho lemoha le likarolo tse ling. Sehlahisoa sa mofuta ona se ka sebelisoa ka mefuta e mengata e fapaneng ea adsorbent, joalo ka BM - GCB depigmentation, BM - PSA ho tlosa protheine le organic acid, BM - NH2 ho tlosoa ha organic acid, BM - WCX hloekisa lintho tsa alkaline, BM - C18 tlosa mafura le lintho tse ling tse phelang, alumina e tlosa mafura, anhydrous magnesium sulfate e tlosa mongobo. e ka thusa ho ntša meriana hamolemo.Mekhoa e fapaneng ea ho bokellana e ka amoheloa ho ikamahanya le litlhoko tse fapaneng tsa kalafo.

Likarolo tsa letoto la BM-Q:
★Sekhahla se phahameng sa ho hlaphoheloa ha lithethefatsi:sekhahla se khotsofatsang sa ho fola bakeng sa boholo ba chefo e bolaeang likokoanyana ea polar.
★Mefuta e mengata ea lisebelisoa:bakeng sa tlhahlobo ea mefuta e fapaneng ea lisampole.
★Mokhoa o bonolo:mokhoa o bonolo, mehato ea ts'ebetso e nyenyane, 'me phello ea lintlha tsa batho mabapi le ho nepahala ha mokhoa o ka fokotseha, ha ts'ebetso ea laboratori e ka ntlafatsoa' me mosebetsi oa laboratori o ka ntlafatsoa.
★Liphetho lia tšepahala:lisebelisoa tsa mantlha tsa lihlahisoa li tsoa ho mofani a le mong, 'me ho tsitsa le ho tsitsa ha sepheo ho ka tšeptjoa haholoanyane.
★Tšireletso ea tikoloho ea sehlahisoa:tšebeliso ea solvent e nyane, ts'ireletso ea tikoloho e hloekileng, ka mor'a ho eketsa solvent, e ka tiisa setshelo hang-hang, ea fokotsa kotsi ho moetsi.

Tlhaloso ea ts'ebetso:

Mohlala oa ts'ebetso:

Ho etsa bonnete ba ho tšoana ha sampuli.Pele ho sebelisoa, lisampole tse tiileng tse kang litholoana le meroho li lokela ho khangoa 'me li be le homogenated,' me mohlala oa meroho le litholoana hangata ke 10-15g, le homogenation ea mochine oa homogenation oa lithane tse phahameng (RPM 11000). r/min-24000 r/min).

Mohato oa 1: mohlala oa tlhahiso:

Palo e lekantsoeng ea sampole e robehileng e ka har'a tube ea BM-Q ea ho ntšoa, e kenyellelitse li-solvents tse loketseng ho BM-Q extraction tube, 'me homogenizing kapa ultrasonic extraction e ne e sebelisetsoa ho hloekisa mokelikeli.

Mohato oa 2: tlhoekiso

Ho ea ka mohlala, khetha phala e loketseng ea BM-Q ea ho hloekisa, ebe u eketsa mokelikeli ka har'a tube ea ho hloekisa, sisinya hantle 'me u kopanye hantle,' me u centrifuge motsoako oa mokelikeli kapa ho lemoha sampuli e tobileng. tsoela pele ho hloekisa mokelikeli oa ho hloekisa ho finyella phello e ntle ea ho hloekisa.

Lintlha:

Ha sampole e kopane ea purge line, anhydrous magnesium sulfate e kanna ea ba le feberu le ketsahalo ea khase, Litlhahiso tsa leqhubu la ho sisinyeha kamora nako e itseng ka mor'a hore ho buloe tube ea centrifuge e hlakotsoeng ho sisinyeha le ts'ebetso e meng.

Ha metsi a nang le metsi a mangata a kenyelletsoa phala ea tlhoekiso e nang le magnesium sulfate, ka kopo eketsa solvent pele o eketsa sampole ho thibela sephetho sa caking ea sampole le magnesium sulfate.

Ka kopo ela hloko ho tiisoa ha sehlahisoa.Ha sehlahisoa se sa phutholoha, se ka koaloa ka mokotleng o tiisitsoeng kapa sa bolokoa sebakeng se omileng kapa se nang le moea o motlakase.

Mathata a teng ka mokhoa oa mantlha oa QuEChERS:

Sebakeng sa asiti (lamunu), sekhahla sa ho khutlisa chefo e bolaeang likokoanyana sa alkaline se tlase; Esita le ka har'a matrix a sa nke lehlakore, chefo e bolaeang likokoanyana e senyehang ka alkaline ea senyeha.

Litharollo:

Ha a ntse a hlahloba acidic medium (orange) ho kopanela ka ntsa HAC le NaAc motsoako, ka boloka pH tharollo tsamaiso pakeng tsa 4 ho 5, e le ho rarolla bothata ba ho hlaphoheloa alkaline chefo e bolaeang likokoanyana ameha.

BM-QUECHERS Lintlha tsa Odara
     Mohlala oa mokhoa oa ho itokisa, o hlahisitsoeng ke Anastassiades ka 2003, o sebelisitsoe haholo ho fumana lijo, mehlala ea metsi a tikoloho le litšila tsa mobu.Lihlahisoa tsa k'hamphani ea rona li sebetsa lefapheng la temo la Chaena, NY/T, AOAC le eu EN.

Letoto lena la lihlahisoa le ka hlokomela ka botlalo kabo ea othomathiki, ho kenya le ho paka phofo, ho hlokomela boiketsetso, sekala le tlhahiso ea bongata, le ho amohela mokhoa o ikhethileng oa motho.

Amohela bareki ba bacha le ba khale ho botsa, ho buisana ka tšebelisano 'moho le ho batla nts'etsopele e tšoanang!