Molao-motheo oa ho ntša mokhahlelo o tiileng

Solid Phase Extraction (SPE) ke mohlala oa thekenoloji ea kalafo e entsoeng esale pele ho tloha bohareng ba lilemo tsa bo-1980. E hlahisoa ke motsoako oa mokelikeli o tiileng le chromatography ea metsi. Haholo-holo e sebelisoa bakeng sa karohano, tlhoekiso le ho ntlafatsa lisampole. Morero oa mantlha ke ho fokotsa tšitiso ea sampole ea matrix le ho ntlafatsa kutloisiso ea ho lemoha.

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Ho ipapisitsoe le thuto ea chromatography e tiileng ea mokelikeli, theknoloji ea SPE e sebelisa papatso e ikhethileng le tlhahiso e ikhethileng ho ntlafatsa, ho arola le ho hloekisa lisampole. Ke ts'ebetso ea ho ntša 'mele ho kenyeletsa le mekhahlelo ea metsi le e tiileng; e ka boela ea lekanyetsoa ka ho nkoa e le mokhoa o bonolo oa chromatographic.
Sets'oants'o sa schematic sa sesebelisoa sa ho ntša karolo e tiileng
SPE ke molao-motheo oa karohano ea mokelikeli oa chromatography o sebelisang adsorption e ikhethileng le tlhahiso e ikhethileng. Mokhoa o sebelisoang haholo ke ho fetisa tharollo ea sampole ea mokelikeli ka har'a adsorbent, ho boloka ntho e lokelang ho lekoa, ebe o sebelisa mokelikeli o matla o loketseng ho ntša litšila, ebe o ntša ka potlako ntho e lokelang ho lekoa ka tekanyo e nyane. solvent, e le ho finyella sepheo sa karohano e potlakileng, ho hloekisa le ho tsepamisa mohopolo. Hape hoa khoneha ho khetha li-adsorb tse sitisang litšila 'me u lumelle ntho e lekantsoeng hore e phalle; kapa ho adsorb litšila le ntho e lekantsoeng ka nako e le 'ngoe, ebe o sebelisa mokelikeli o loketseng ho khetha ntho e lekantsoeng.
The extractant ea mokhoa o tiileng-mohato oa ho ntša o tiile, 'me molao-motheo oa eona oa ho sebetsa o itšetlehile ka taba ea hore likarolo tse lokelang ho lekanyetsoa le likarolo tse ngata tse sitisang ka har'a sampole ea metsi li na le matla a fapaneng holim'a moemeli oa mokhahlelo o tiileng, e le hore ba. ba arohane ho e mong. Solid phase extraction agent ke filler e khethehileng e nang le C18 kapa C8, nitrile, amino le lihlopha tse ling.


Nako ea poso: Jun-14-2022