Nucleic Acid Extraction System (Nucleic Acid Extraction System) ke sesebelisoa se sebelisang li-reagents tse ts'oanang tsa nucleic acid ho phethela sampole ea nucleic acid ka bo eona. E sebelisoa haholo litsing tsa taolo ea mafu, tlhahlobo ea mafu a kliniki, polokeho ea tšelo ea mali, boitsebahatso ba forensic, tlhahlobo ea likokoana-hloko tsa tikoloho, tlhahlobo ea polokeho ea lijo, leruo la liphoofolo le lipatlisiso tsa baeloji ea limolek'hule le likarolo tse ling.
1. Mokhoa oa ho monya, oo hape o tsejoang e le mokhoa oa pipetting, ke ho ntša nucleic acid ka ho thibela lifaha tsa makenete le ho fetisa mokelikeli. Ka kakaretso, phetiso e etsoa ka ho laola letsoho la robotiki ka sistimi e sebetsang. Mokhoa oa ho phunya o tjena:

1) Lysis: Eketsa tharollo ea lysis ho sampuli, 'me u hlokomele ho kopanya le karabelo e feletseng ea tharollo ea karabelo ka ho sisinyeha ha mochine le ho futhumatsa, lisele li lysed,' me nucleic acid e lokolloa.

2) Adsorption: Eketsa lifaha tsa makenete ho sampole ea lysate, kopanya hantle, 'me u sebelise lifaha tsa makenete ho ba le kamano e matla bakeng sa li-nucleic acids tlas'a letsoai le phahameng le pH e tlase ho adsorb nucleic acid. Tlas'a ketso ea matla a khoheli a kantle, likhahla tsa makenete li arohane le tharollo. , sebelisa ntlha ho ntša mokelikeli le ho o lahlela ka tanka ea litšila, 'me u lahle ntlha eo.

3) Ho hlatsoa: Tlosa matla a khoheli a kantle, o nke sebaka ka ntlha e ncha, 'me u kenye bafa ea ho hlatsoa, ​​kopanya hantle ho tlosa litšila,' me u tlose mokelikeli tlas'a ts'ebetso ea matla a khoheli a kantle.

4) Elution: Tlosa matla a khoheli a kantle, o nke sebaka ka ntlha e ncha, eketsa buffer ea elution, kopanya hantle, ebe o arola nucleic acid e tlanngoeng ho lifaha tsa makenete ho fumana asiti e hloekisitsoeng ea nucleic.
2. Magnetic bar mokhoa

Mokhoa oa magnetic rod o hlokomela karohano ea li-nucleic acid ka ho lokisa mokelikeli le ho fetisetsa lifaha tsa makenete. Molao-motheo le ts'ebetso li ts'oana le tsa mokhoa oa ho hula, empa phapang ke mokhoa oa karohano lipakeng tsa lifaha tsa makenete le mokelikeli. Mokhoa oa "magnetic bar" ke ho arola lifaha tsa makenete ho tsoa ho mokelikeli o litšila ka ho kenella ha molamu oa makenete ho ea ho lifaha tsa makenete, ebe o li kenya ka har'a mokelikeli o latelang ho hlokomela ho ntšoa ha nucleic acid.