Karohano le tlhoekiso ea liprotheine e sebelisoa haholo lipatlisisong le ts'ebetsong ea biochemistry mme ke tsebo ea bohlokoa ea ts'ebetso. SCG Protein Purification System Company-Saipu Instrument e hlophisitse karohano e litšila le karohano e ntle eaprotheinetlhoekiso bakeng sa bohle. Sele e tloaelehileng ea eukaryotic e ka ba le likete tsa liprotheine tse fapaneng, tse ling li ruile haholo 'me tse ling li na le likopi tse seng kae feela. E le hore u ithute protheine e itseng, ho hlokahala hore u hloekise protheine pele ho liprotheine tse ling le limolek'hule tse seng tsa protheine.

Karohano e mahoashe

Ha protheine extract (ka linako tse ling e kopantsoe le nucleic acids, polysaccharides, joalo-joalo) e fumanoa, ho khethoa sete ea mekhoa e loketseng ho arola tse lakatsehang.protheineho tsoa litšila tse ling. Ka kakaretso, mohato ona oa karohano o sebelisa mekhoa e joalo ka salting out, isoelectric point accumulation le organic solvent fractionation. Mekhoa ena e khetholloa ka mokhoa o bonolo le matla a maholo a ho sebetsa, a ka tlosang litšila tse ngata le ho tsepamisa mohopolo tharollo ea protheine. Li-extracts tse ling tsa protheine li kholo ka bongata 'me ha li tšoanelehe ho tsepamisoa ka ho bokella kapa ho ntša letsoai. U ka khetha ultrafiltration, gel filtration, ho omisa vacuum e batang kapa mekhoa e meng ea ho tsepamisa mohopolo.

Karohano e ntle

Ka mor'a ho aroloa ho thata ha sampuli, molumo hangata o monyenyane, 'me boholo ba litšila bo tlosoa. Bakeng sa tlhoekiso e tsoelang pele, mekhoa ea chromatography ka kakaretso e kenyelletsa tlhoekiso ea gel, chromatography ea phapanyetsano ea ion, chromatography ea adsorption, le affinity chromatography. Haeba ho hlokahala, u ka boela khetha electrophoresis, ho akarelletsa le zone electrophoresis, isoelectric point set, joalo-joalo e le mokhoa oa ho qetela oa tlhoekiso. Mokhoa o sebelisoang bakeng sa karohano ea boemo ba karohano ka kakaretso o nyane ho rala, empa ka tharollo e phahameng.

Crystallization ke mokhoa oa ho qetela oa karohano ea liprotheine le tlhoekiso. Le hoja ts'ebetso ea crystallization e sa etse bonnete ba hore protheine e tlameha ho tšoana, ke feela ha protheine e itseng e na le molemo ka tharollo ea ho etsa kristale. Ts'ebetso ea crystallization ka boeona e tsamaisana le tekanyo e itseng ea tlhoekiso, 'me recrystallization e ka tlosa palo e nyenyane ea protheine e silafetseng. Ho tloha ka denaturedprotheineha ho mohla e kileng ea fumanoa nakong ea ts'ebetso ea crystallization, protheine crystallization hase feela pontšo ea bohloeki, empa hape ke tataiso e matla ea ho fumana hore sehlahisoa se boemong ba sona ba tlhaho.