Karohano le tlhoekiso ea liprotheine e sebelisoa haholo lipatlisisong le ts'ebetsong ea biochemistry mme ke tsebo ea bohlokoa ea ts'ebetso. Sele e tloaelehileng ea eukaryotic e ka ba le likete tsa liprotheine tse fapaneng, tse ling li ruile haholo 'me tse ling li na le likopi tse seng kae feela. E le hore u ithute ntho e itsengprotheine, hoa hlokahala ho qala ho hloekisa protheine ho liprotheine tse ling le limolek'hule tse seng tsa protheine.

1. Mokhoa oa ho ntša letsoai oaprotheine:

Letsoai le sa nke lehlakore le na le phello e kholo ho solubility ea protheine. Ka kakaretso, ka ho eketseha ha letsoai tlas'a letsoai le tlaase, ho qhibiliha ha protheine hoa eketseha. Sena se bitsoa salting; ha letsoai le ntse le eketseha, ho qhibiliha ha protheine ho fokotseha ho ea ka likhato tse sa tšoaneng 'me ho arohana ka ho latellana. Ketsahalo ena e bitsoa salting out.

2. Mokhoa oa ho bokella lintlha tsa Isoelectric:

The electrostatic repulsion between particles ke e nyenyane ka ho fetisisa ha protheine e sa sisinyehe, kahoo solubility le eona ke e nyenyane ka ho fetisisa. Lintlha tsa isoelectric tsa liprotheine tse fapaneng li fapane. PH ea tharollo ea boemo e ka sebelisoa ho fihlela ntlha ea isoelectric ea protheine E etsa hore e bokelle, empa mokhoa ona o sebelisoa ka seoelo o le mong 'me o ka kopanngoa le mokhoa oa ho tsoa letsoai.

3. Dialysis le ultrafiltration:

Dialysis e sebelisa lera la semi-permeable ho arola liprotheine tsa boholo bo fapaneng ba limolek'hule. Mokhoa oa ultrafiltration o sebelisa khatello e phahameng kapa matla a centrifugal ho etsa hore metsi le limolek'hule tse ling tse nyane tsa solute li fete ka har'a lesela le nang le semi-permeable, haprotheinee sala holim'a lera. U ka khetha boholo bo fapaneng ba pore ho thibela liprotheine tsa boima bo fapaneng ba limolek'hule.

Mokhoa oa ho hloekisa 4.Gel:

E boetse e bitsoa size exclusion chromatography kapa molecular sieve chromatography, ona ke o mong oa mekhoa e sebetsang haholo ea ho arola metsoako ea protheine ho latela boholo ba limolek'hule. Lisebelisoa tsa ho paka tse sebelisoang haholo kholomong ke gel ea tsoekere (Sephadex ged) le gel ea agarose (gel ea agarose).

5. Electrophoresis:

Tlas'a boemo bo tšoanang ba pH, liprotheine tse fapaneng li ka aroloa ka lebaka la boima ba tsona bo fapaneng ba limolek'hule le litefiso tse fapaneng tšimong ea motlakase. Ho bohlokoa ho ela hloko electrophoresis ea isoelectric, e sebelisang ampholyte joalo ka sejari. Nakong ea electrophoresis, ampholyte e etsa pH gradient butle-butle e eketsoa ho tloha ho electrode e ntle ho ea ho electrode e mpe. Ha protheine e nang le tefiso e itseng e sesa ho eona, e tla fihla ho e mong. Boemo ba pH ba ntlha ea motlakase ha bo tsoelepele, 'me mokhoa ona o ka sebelisoa ho sekaseka le ho lokisa liprotheine tse fapaneng.

6.Ion puisano chromatography:

mahlahana a puisano a ion a kenyelletsa mahlahana a puisano a cationic (joalo ka carboxymethyl cellulose; CM-cellulose) le mahlahana a puisano a anionic (diethylaminoethyl cellulose). Ha o feta kholomong ea puisano ea ion chromatography, protheine e nang le tefiso e fapaneng le moemeli oa puisano ea ion e adsorbed ho moemeli oa puisano oa ion, ebe e adsorbed.protheinee hlahisoa ka ho fetola pH kapa matla a ionic.

7. Affinity chromatography:

Affinity chromatography ke mokhoa o sebetsang haholo oa ho arola liprotheine. Hangata ho hloka mohato o le mong feela ho arola protheine e itseng ho hloekisoa motsoako o litšila oa protheine o nang le bohloeki bo phahameng.

Mokhoa ona o ipapisitse le ho tlamahanngoa ha liprotheine tse itseng ho molek'hule e 'ngoe e bitsoang ligand (Ligand).

Molao-motheo oa motheo:

Liprotheine li teng ka har'a motsoako o bohlasoa oa lisele kapa lisele, 'me mofuta o mong le o mong oa sele o na le liprotheine tse fapaneng tse likete. Ka hona, phapang pakeng tsa liprotheine ke karolo ea bohlokoa ea biochemistry, 'me ha ea ka ea e-ba eona feela. Kapa sete ea mekhoa e lokiselitsoeng e ka tlosa mofuta ofe kapa ofe oa protheine ho tsoa ho protheine e kopantsoeng e litšila, kahoo mekhoa e mengata e atisa ho sebelisoa hammoho.