Mekhoa eatlhoekiso ea protheine:

Mokhoa oa ho hloekisa liprotheine, karohano le tlhoekiso ea protheine, protheine e lokolloa liseleng tsa pele kapa lisele tse boemong bo qhibilihileng 'me e lula e le boemong ba tlhaho ntle le tahlehelo ea ts'ebetso ea likokoana-hloko. Ka lebaka lena, thepa e lokela ho tlosa nama ea liphoofolo le adipose, lihlahisoa tsa peo li lokela ho tlosa tšilafalo ea seaparo sa peo ho tsoa linthong tse kang li-tannins, 'me peo ea oli e lokela ho sebelisoa ka ho khetheha ho sebelisa motsoako o tlaase o belang.

1. Mokhoa oa ho hloekisa liprotheine, boholo le mekhoa e fapaneng ea ho hlahloba karohano: dialysis le ultrafiltration li sebelisa liprotheine tsena ho feta ka tlhaho ea limolek'hule tse ke keng tsa feta ka har'a lera la semi-permeable; density gradient centrifugation Boleng le boima ba protheine bohareng bo tla ba le tšusumetso e kholo ho likaroloana. Lebelo la ho lokisa le potlakile; filtration ea gel ke mofuta oa chromatography ea kholomo.

2. Karohano ea solubility phapang e sebelisoang: electro-precipitation hobane letlooa tefiso ea limolek'hule protheine ke lefela isoelectric ntlha, ho fokotsa repulsion electrostatic pakeng tsa limolek'hule, bonolo aggregate, le bonyane solubility; motsoako oa letsoai o sebelisoang ka motsoako oa letsoai Motsoako o eketsa kapa o fokotsa ho qhibiliha ha protheine.

3. Tefiso e arohane le ho hlahlojoa ka mekhoa e sa tšoaneng, litaba tse ka sehloohong li kenyelletsa electrophoresis le ion exchange chromatography.

4. Mekhoa eatlhoekiso ea protheinePhatlalatso e ikhethileng le karohano ea liprotheine li sebelisa matla a phallo ea likaroloana.

5. Affinity chromatography e itšetlehile ka ho arohana ha li-ligands. Tšobotsi ena ea baeloji e lumella limolek'hule tsa protheine hore li tlamahane ka ho khetheha ho e-na le ho tlama molek'hule e 'ngoe e bitsoang ligand.

6. Mokhoa oa tlhoekiso ea liprotheine: Mocheso o tlase oa organic solvent precipitation method: Sebelisa li-solvents tse tsoakaneng le metsi, tse kang methanol, ethanol, ho fokotsa ho qhibiliha le ho na ha liprotheine tse ngata. Mokhoa ona o phahame ho feta tharollo ea salting, empa protheine e fokotseha haholo Ha e le bonolo, e lokela ho etsoa ka mocheso o tlase.

Mokhoa oa ho hloekisatlhoekiso ea protheine:

Mokhoa oa ho hloekisa protheine, protheine ke molek'hule e kholo, le boholo bo fapaneng ba limolek'hule tsa albumin, ho na le mekhoa e meng e bonolo ea ho arola liprotheine le lisebelisoa tse nyenyane tsa molek'hule, hape le motsoako oa protheine o arohaneng. Mekhoa ea mantlha ea ho arola liprotheine tsa boholo bo fapaneng ba limolek'hule ke dialysis, ultrafiltration, filtration ea gel, le karohano ea centrifugal. Dialysis le ultrafiltration li sebelisoa hangata mekhoeng ea karohano ea liprotheine. Motsoako oa dialysis o lokelang ho aroloa o kenngoa ka mokotleng oa dialysis o entsoeng ka lera le khonang ho phunyeletsa, ebe o qoelisoa ka tharollo ea dialysis bakeng sa karohano. Ultrafiltration ke ts'ebetso ea ho fetisa metsi le limolek'hule tse ling tse nyane ho sebelisa matla a centrifugal kapa khatello ka har'a lera le nang le semi-permeable le ho ts'oara liprotheine ka har'a lera la semi-permeable. Mekhoa ena e 'meli e ka aroloa ho li-macromolecules tse thehiloeng ho protheine le limolek'hule tse nyane tse thehiloeng letsoai. Hangata li ntšoa ka letsoai, tse sebelisoang hammoho le mokhoa oa ho qhibiliha letsoai, kapa li ka hlahisoa ho ntša mekhoa ena e 'meli ka mor'a hore letsoai le sebelisoe ho sebelisa metsoako ea letsoai le inorganic.

1. Mokhoa oa ho hloekisa protheine ka mokhoa oa ho tsoa letsoai: Motheo oa mokhoa oa letsoai ke hore ho qhibiliha ha protheine ka har'a motsoako oa letsoai ho tla eketseha ka keketseho ea letsoai, empa ha letsoai le eketseha ho fihlela boleng bo itseng. ts'ebetso ea metsi e tla eketseha. Ho fokotseha, ho ka etsang hore liprotheine tse ling li hlahise litefello ka holim'a sebopeho sa limolek'hule hore li fokotsehe butle-butle, 'me lera la hydration le senyeha butle-butle, e leng se ka qetellang se etsa hore liprotheine le limolek'hule tse amanang le tsona li be le agglomeration le precipitate ho tsoa litharollo tse fapaneng.

2. Mokhoa oa ho noa ha li-solvents: Ho na le mabaka a mabeli a ho fokotsa ho qhibiliha ha protheine ho li-solvents: mokhoa oa ho hloekisa protheine e le tharollo ea letsoai ka ho felloa ke metsi; ea bobeli, dielectric constant of organic solvents ho feta metsi a ka fokotswa Ka solvents polar.

3. Protein precipitating Agent: protheine e le precipitating agent e sebetsa feela mofuteng o le mong kapa o mong oa liprotheine tsa pula, tse tloaelehileng ke alkaline, protheine, lectin, le lirafshoa tse boima.

4. Polyethylene glycol precipitation: Mokhoa oa ho hloekisa li-polymer tse sa qhibilihang ka metsing tse se nang ionic bakeng satlhoekiso ea protheine, joalo ka polyethylene glycol le sodium dextran sulfate ho fokotsa liprotheine.