Ts'ebetso e akaretsang ea ho tlosoa ha karolo e tiileng ke e latelang:

1. Ho sebelisa adsorbent: Hlatsoa cartridge e tsitsitseng ea ho ntša karolo e tiileng ka solvent e loketseng pele u ntša sampuli ho boloka adsorbent e le metsi, e ka khonang ho hlahisa metsoako ea sepheo kapa metsoako e sitisang. Mefuta e fapaneng ea ts'ebetso e tiileng ea khatriji ea ho ntša cartridge e sebelisa li-solvents tse fapaneng:

(1) Li-adsorbents tse fokolang tsa polar kapa tse se nang polar tse sebelisoang ho reverse-phase solid-phase extraction hangata li hlatsuoa ka mokelikeli o qhibilihang ke metsi, o kang methanol, ebe o hlatsuoa ka metsi kapa tharollo ea buffer. Hape hoa khoneha ho hlatsoa ka solvent e matla (e kang hexane) pele u hlatsoa ka methanol ho felisa litšila tse adsorbent le ho kena-kenana ha tsona le motsoako o lebisitsoeng.

(2) The polar adsorbent sebelisoa ka tloaelo-mohato solid-phase extraction hangata eluted le organic solvent (sample matrix) moo ho lebisitsoe motsoako teng.

(3) The adsorbent sebelisoa ka ion-exchange solid phase extraction ka hlatsuoa le sampole solvent ha e sebelisoa bakeng sa disampole ka non-polar organic solvents; ha e sebelisoa bakeng sa lisampole ka lihlapolli tsa polar, e ka hlatsuoa ka li-solvents tse qhibilihang ka metsing Ka mor'a hore e hlatsoe, e hlatsoe ka tharollo e nang le metsi e nang le pH e loketseng le e nang le metsoako e itseng ea tlhaho le matsoai.

E le ho boloka sorbent ka har'a cartridge ea SPE e kolobile ka mor'a ts'ebetso le pele ho kenyelletso ea sampole, hoo e ka bang 1 ml ea solvent bakeng sa ts'ebetso e lokela ho bolokoa ho sorbent ka mor'a hore e sebelisoe.

2. Sample loading: Tšela mokelikeli kapa sampuli e qhibilihisitsoeng e tiileng ka har'a cartridge e tsitsitseng e tiileng ea ho ntša, ebe u sebelisa vacuum, khatello kapa centrifugation ho etsa hore sampuli e kene ka har'a adsorbent.

3. Ho hlatsoa le ho hlatsuoa: Ka mor'a hore sampuli e kene ka har'a adsorbent 'me motsoako o lebisitsoeng o adsorbed, motsoako o fokolang o bolokiloeng o ka hlatsuoa ka mokelikeli o fokolang,' me joale motsoako o lebisitsoeng o ka tlosoa ka motsoako o matla le ho bokelloa. . Rinse le Elution Joalo ka ha ho hlalositsoe pejana, ntho e sa tloaelehang e ka fetisoa ka adsorbent ka vacuum, khatello kapa centrifugation.

Haeba adsorbent e khethiloe hore e be le bofokoli bo fokolang kapa bo se nang li-adsorption ho motsoako o lebisitsoeng le ho ba le papatso e matla ho motsoako o sitisang, motsoako o lebisitsoeng o ka boela oa hlatsuoa 'me oa bokelloa pele, ha motsoako o sitisang o bolokiloe (adsorption). ) ho adsorbent, tse peli li arohane. Maemong a mangata, sepheo sa motsoako se bolokiloe ho adsorbent, 'me qetellong se e-na le solvent e matla, e leng ho loketseng haholoanyane ho hloekisa sampuli.