Blot Analysis in Biopharmaceutical, Medical and Other Fields
"Leano la 14th la Lilemo Tse Hlano" Leano la Ntlafatso ea Moruo oa Boipheliso le sisinya hore moruo oa tikoloho o lokela ho tsamaisoa ke nts'etsopele le tsoelopele ea mahlale a bophelo le theknoloji ea baeloji, e ipapisitseng le ts'ireletso, nts'etsopele le ts'ebeliso ea lisebelisoa tsa baeloji, 'me e ipapisitse le kopanyo e pharaletseng le e tebileng. meriana, bophelo bo botle, temo, meru, le matla. , tšireletso ea tikoloho, thepa le liindasteri tse ling; ho hlakile hore ntshetsopele ya moruo wa baeloji ke tataiso ya bohlokwa ya ho ikamahanya le tlwaelo e potlakisang ya ho iphetola ha dintho ya lefatshe ka bophara le ho fihlella boitshepo ba maemo a hodimo ba saense le thekenoloji. Ke mohato oa bohlokoa ho hlaolela le ho holisa indasteri ea bioloji le ho khothaletsa nts'etsopele ea maemo a holimo ea moruo. Ho kopana le kgolo e potlakileng ya ditlhoko tsa bophelo le tsa bophelo bo botle le ho kgotsofatsa tabatabelo ya batho ya bophelo bo betere ke tiisetso ya bohlokwa bakeng sa ho matlafatsa thibelo le taolo ya naha ya dikotsi tsa biosecurity le ho kgothaletsa ntjhafatso ya tsamaiso ya naha ya puso le bokgoni ba puso.
E le ho arabela pitso ea naha, BM e ikemiselitse ho hlōla theknoloji ea tlhahiso ea lifilimi ea boemo bo phahameng le ho lemoha butle-butle ho kenya sebaka sa thepa ea boleng bo phahameng lefapheng la saense ea bophelo. Ka Mots'eanong 2023, tlhahiso e kholo ea li-membrane tsa immunochromatography NC e ile ea finyelloa ka katleho mme ea sebelisoa ho li-reagents tse fapaneng tsa ho lemoha kapele. Hajoale, filimi ea NC e se e sebelisitsoe tlhahlobong ea in vitro ea malapeng, polokeho ea lijo, tlhahlobo e potlakileng ea lithethefatsi le likarolo tse ling, 'me e fihletse thepa e kantle ho naha mme e qothisana lehlokoa le linaha tse kholo tsa' marakeng! Kamora ho phethela puo ea 'maraka oa lifilimi oa NC, kamora likhoeli tse' maloa tsa lipatlisiso tsa tekheniki ke sehlopha sa rona sa botekgeniki, ho arabela litlhoko tse potlakileng tsa basebelisi lefapheng la mahlale a bophelo lefatšeng ka bophara ho fokotsa litšenyehelo tsa lisebelisoa tsa boleng bo holimo, re ile ra qala ka katleho li-membrane tsa blotting. , e loketseng biopharmaceuticals, meriana le masimo a mang. Tlhahlobo ea blot ea Bophirimela (Western Blotting, WB)
Kenyelletso ea likarolo tsa BM Blotting Membranes, : Boholo ba pore le mofuta oa protheine e sebetsang 0.1μm e loketseng liprotheine tse nang le boima ba molek'hule ka tlase ho 7kDa 0.22μm tse loketseng liprotheine tse nang le boima ba molek'hule ka tlase ho 20kDa 0.45μm tse loketseng liprotheine tse boima ba molek'hule tse kholo ho feta 20kDa Main Melao-motheo e tlamang liprotheine Motlakase o tsitsitseng le hydrophobicity Maemo a sebetsang a phetisetso le mekhoa ea ho lemoha Chemiluminescence Ho lemoha ha Fluorescence ka radiolabeled probe ho daea ka ho otloloha ho daea Sesole sa 'mele se hokahaneng le Enzyme Molemo :
1.Low Background, high sensitivity
2.Ha ho tlhokahalo ea reagent ea joala pele ho metsi
3.Sebopeho se ikhethang sa holim'a metsi le thepa e etsa hore ho be le tekanyo e babatsehang ea pontšo-ho-lerata Thepa e tsoa ho likhoele tsa tlhaho, e na le tikoloho, 'me e ka boloka protheine e tlamiloeng e sebetsa ka nako e telele.
Selelekela sa theknoloji ea tlhahlobo ea WB Theknoloji ea tlhahlobo ea WB ke theknoloji e sebelisoang haholo mafapheng a biology ea limolek'hule, biochemistry, immunology le mafapha a mang. Theknoloji ena e sebelisa tlamahano e khethehileng ea li-antibodies ho liprotheine tse itseng tse ka har'a lisele kapa lisampole tsa lisele ho fihlela boitsebahatso ba protheine le tlhahlobo ea polelo e ipapisitseng le boemo le matla a sehlopha sa mebala, ke hore, tlhahlobo ea boleng le karolo ea boholo. E ile ea hlahisoa ka lekhetlo la pele ke Harry Towbin oa Friedrich Miescher Institute Switzerland ka 1979. E se e le lilemo tse fetang 40 tse fetileng 'me e se e le mokhoa oa khale haholo le o sebetsang oa ho etsa lipatlisiso tsa protheine.
Nako ea poso: Feb-02-2024
