---" Ultrafiltration & Microfiltration" Ujeedo badan, garashada beddelka gudaha!
BM Sayniska Nolosha,Ultrafiltration Tube Centrifugal (Filter Centrifugal)Alaabooyinka Taxanaha ah
Waxay ka kooban tahay tuubo filter gudaha ah (xub leh) + tuubo centrifuge dibadda ah, kaas oo si dhakhso ah oo waxtar leh u nadiifin kara oo u ururin kara ilaa 15ml oo muunado noole ah. Naqshad toosan oo gaar ah iyo aagga shaandhaynta la kordhiyo waxay ku siin kartaa muunad deg-deg ah awoodda farsamaynta iyo heerka soo kabashada muunadda sare (sida caadiga ah ≥90% xalinta asalka ah), iyadoo la ilaalinayo shaandhaynta khafiifka ah iyo jawi fiirsashada si loo ilaaliyo dhaqdhaqaaqa borotiinka iyo Conformation. Solute oo si weyn u yareeya wasakhda xuubka shaandhada ee ay keento centrifugation-ka xiga. Marka la wanaajiyo barta joogsiga shaandhada shaandhada ee aaladda shaandhada, waxay ka ilaalin kartaa shaandhada centrifugal inay qallajiso muunadda ama ay keento muunadda luminta wareegga xad-dhaafka ah awgeed. . Noocyada kala duwan ee ultrafiltration ultrafiltration molecular cutoff (MWCO) filtarrada centrifugal waxay adeegsadaan nadiif sare, xayaysiin hooseeya PES iyo xuubka RCE, kuwaas oo leh borotiin aad u hooseeya iyo heerarka isku xidhka aashitada nucleic.
BM Life Science, wax soo saarka qolka nadiif ah ee dhammaan xiriiradaUltrafiltration Tube Centrifugal (Filter Centrifugal), Hawlgalka khadka isku dhafka ah, maamulka ERP oo buuxa, alaabada aadka u saafiga ah, DNase/RNase ma jiro, ma laha PCR-horjoogayaasha, ma laha il kulayl. Sayniska Nolosha BM, dhammaan cabbirradaUltrafiltration Tube Centrifugal (Filter Centrifugal)alaabooyin taxane ah ayaa la habeeyey, taxanaha alaabadu waa kuwo xasiloon oo isku dhafan oo leh faraqa ugu yar ee u dhexeeya dufcadaha iyo tayada sare. Waxaa lagu bedeli karaa wax soo saarka gudaha waxaana loo dhoofin karaa Japan, South Korea, Europe, iyo Maraykanka. Si ballaaran loo isticmaalo kala-saaridda muunadda iyo tijaabooyinka sifaynta caadiga ah.
Codsiga
1 UltrafiltrationBeddelka kaydinta, nadiifinta, fiirsashada, iyo nadiifinta antigens, unugyada difaaca jirka, enzymes, iyo borotiinnada kale, iyo sidoo kale muunadaha noolaha sida asiidhka nucleic iyo microorganisms, nadiifinta qaybaha chromatographic, kala soocida molecules noolaha ee dhaqanka unugyada ama xalalka lysis unugga, saarista ee aasaasayaasha, isku xidhayaasha, ama calaamadaha molecular ee isku darka falcelinta, feejignaanta fayraska ama kala soocida, iyo kala soocida cayriin ee isku darka molecule noolaha.
2 Microfiltrationkala saara asiidhyada nucleic-ka ah iyo borotiinada ku jira jeelka, muunado ayaa la sifeeyaa oo la caddeeyaa, waxaana la ururiyaa oo la dhaqaa qaybo warshadaysan ama tusbaxyada.
Tilmaamaha Alaabta
★Saamiga feejignaanta sare, kaas oo si fudud u gaari kara 80-100 jeer saamiga fiirsashada;
★Xawaaraha fiirsiga muunadku waa dhakhso, wakhtiga fiirsashada guudna waa 10-60 daqiiqo. Waxa kale oo ay leedahay nakhshad quful ka-hortagga qallajinta si looga fogaado waxyeellada muunadda sababtoo ah centrifugation xad-dhaaf ah;
Heerka soo kabashada muunadku aad ayuu u sarreeyaa wuxuuna gaadhi karaa heerka soo kabashada in ka badan 90%;
Xayeysiis hooseeya, xuubka PES/RC iyo naqshadeynta gidaarka gudaha ee siman ayaa leh xayeysiis borotiin ah oo aad u hooseeya iyo heerka isku xirka biomolecule;
4ml/15ml,15ml/50ml
Tilmaamo dhammaystiran, tuubooyinka centrifuge ultrafiltration leh laba mug oo 4ml/15ml iyo 15ml/50ml, oo leh cabbirro dalool kala duwan, oo laga heli karo 3KD, 5KD, 10KD, 30KD, 50KD iyo 100KD si ay u buuxiyaan shuruudaha kala duwan ee miisaanka kelli
Hawlgalka ku habboon, dhammaan habka kala soocida iyo sifaynta ayaa lagu dhammayn karaa aCentrifuge Multifunctional, fududahay in lagu shaqeeyo;
Alaabta ayaa nadiif ah, dhammaan isku xirka waxaa lagu soo saaraa qol nadiif ah, hawlgalka khadka shirarka, maamulka ERP oo buuxa, alaabtu waa ultra-sadiif, ma jiro DNase/RNase, ma jiro PCR inhibitor, ma laha il kulayl;
★ Qeexitaan sare, oo aan lahayn asal iyo auto-fluorescence, oo ma saameynayso natiijooyinka ogaanshaha;
★ Waxqabad la isku halayn karo, xidhidh wanaagsan, oo aan ka falcelinayn shaybaarada tijaabada ah, natiijooyinka tijaabada ah waa kuwo la isku halayn karo, waxayna u adkeysan karaan heerkul sare oo 100 ℃ ah!
★ Waxay leedahay codsiyo kala duwan. Codsiyada kala duwan, laba nooc oo ahUltrafiltration Tube Centrifugal (Filter Centrifugal)alaabooyin taxane ah, 4ml / 15ml iyo 15ml / 50ml ayaa loo soo bandhigay si ay u daboolaan baahiyaha macaamiisha kala duwan, aqbalaadda habeynta iyo horumarinta shaqeynta!
Macluumaadka Dalabka
Bisad.Maya | Magaca | Tilmaamid | Sharaxaada | Kumbuyuutar/pk |
UCT015RCE004003 | Ultrafiltration Centrifugal Tube | 4/15ml, 3kD | Xubnaha RCE | 15 pcs/bac |
UCT015RCE004010 | Ultrafiltration Centrifugal Tube | 4/15ml, 10kD | Xubnaha RCE | 15 pcs/bac |
UCT015RCE004030 | Ultrafiltration Centrifugal Tube | 4/15ml, 30kD | Xubnaha RCE | 15 pcs/bac |
UCT015RCE004100 | Ultrafiltration Centrifugal Tube | 4/15ml, 100kD | Xubnaha RCE | 15 pcs/bac |
UCT050RCE015003 | Ultrafiltration Centrifugal Tube | 15/50ml, 3kD | Xubnaha RCE | 24 pcs/bac |
UCT050RCE015010 | Ultrafiltration Centrifugal Tube | 15/50ml, 10kD | Xubnaha RCE | 24 pcs/bac |
UCT050RCE015030 | Ultrafiltration Centrifugal Tube | 15/50ml, 30kD | Xubnaha RCE | 24 pcs/bac |
UCT050RCE015100 | Ultrafiltration Centrifugal Tube | 15/50ml, 100kD | Xubnaha RCE | 24 pcs/bac |
UCT050PES015005 | Ultrafiltration Centrifugal Tube | 15/50ml, 5kD | Xubnaha PES | 12 pcs/bac |
UCT050PES015010 | Ultrafiltration Centrifugal Tube | 15/50ml, 10kD | Xubnaha PES | 12 pcs/bac |
UCT050PES015030 | Ultrafiltration Centrifugal Tube | 15/50ml, 30kD | Xubnaha PES | 12 pcs/bac |
UCT050PES015050 | Ultrafiltration Centrifugal Tube | 15/50ml, 50kD | Xubnaha PES | 12 pcs/bac |
UCT050PES015100 | Ultrafiltration Centrifugal Tube | 15/50ml, 100kD | Xubnaha PES | 12 pcs/bac |
CF050PES015C010 | Shaandhaynta Centrifugal | 15/50ml, 0.1um | 0.1um, PES Membrane | 12 pcs/bac |
CF050PES015C020 | Shaandhaynta Centrifugal | 15/50ml, 0.2um | 0.2um, PES Membrane | 12 pcs/bac |
CF050PES015C045 | Shaandhaynta Centrifugal | 15/50ml, 0.45um | 0.45um, Xubnaha PES | 12 pcs/bac |
UCT** | Ultrafiltration Centrifugal Tube | Habaynta | Habaynta | ** pcs/bac |
CF** | Shaandhaynta Centrifugal | Habaynta | Habaynta | ** pcs/bac |
Sayniska Nolosha ee BM, Ultrafiltration Centrifugal Tube (Centrifugal Filter) cabbiraadaha taxanaha ah iyo xogta tijaabada
Ultrafiltration Centrifugal Tube (Centrifugal Filter) taxanaha waxqabadka alaabta iyo xogta tijaabada ah
Ultrafiltration Centrifugal of samples:
5 KD 0.25mg/ml Cyt C;
10 KD1mg/ml OVA;
30/50 KD 1mg/ml BSA;
100 KD 1mg/ml IgG.
Xaaladaha tijaabada ah: rotor siman, xoogga centrifugal 4000 × g, 8 ℃, mugga bilawga ah 15ml.
Ultrafiltration Centrifugal of samples:
5 KD 0.25mg/ml Cyt C;
10 KD 1mg/ml OVA;
30/50 KD 1mg/ml BSA;
100 KD 1mg/ml IgG.
Xaaladaha tijaabada ah: rotor xagal go'an, xooga centrifugal 5000 × g, 8℃, mugga bilawga ah 12ml.
Heerarka diidmada caadiga ah ee heerarka miisaanka unugyada borotiinka (4000g x 15ml bilowga mugga rotor ruxida ama 5000g x 12ml bilowga mugga rotor xagal go'an)
Heerarka miisaanka molecular/fiirsashada | miisaanka kelli | NMWL Ultrafiltration tube | Heerarka diidmada % Deji baaldiga | Heerarka diidmada % xagal go'an | waqtiga centrifugation |
fitamiin B-12 | 1350 | 3K | 25 | 25 | 60 |
(0.25mg/ml) cytochrome C | 12400 | >95 | >95 | 60 | |
(1 mg/ml) α-Cymotrypsinogen | 25000 | >95 | >95 | 60 | |
B-12 (0.2mg/ml) vitamin | 1350 | 10K | 5 | 5 | 30 |
0.25mg/ml) cytochrome C | 12400 | >95 | >95 | 30 | |
(1 mg/ml) α-Cymotrypsinogen | 25000 | >95 | >95 | 30 | |
Vitamin B-12 (0.2mg/ml) | 1350 | 30K | 5 | 5 | 20 |
Cytochrome C 0.25mg/ml | 12400 | 10 | 10 | 20 | |
Ovalbumin (1 mg/ml) | 45000 | >95 | >95 | 20 | |
BSA (1 mg/ml) | 67000 | >95 | >95 | 20 | |
Cytochrome C (0.25mg/ml) | 12400 | 50K | 5 | 5 | 10 |
Ovalbumin (1 mg/ml) | 45000 | 65 | 55 | 10 | |
BSA (1 mg/ml) | 67000 | >90 | >90 | 10 | |
Ovalbumin (1 mg/ml | 45000 | 100K | 25 | 15 | 20 |
IgG (1 mg/ml) | 156000 | >90 | >90 | 20 | |
Thyroglobulin (0.5mg/ml) | 677000 | >90 | >90 | 20 |
Heerka soo kabashada
Awoodda xajinta ee 15ml xuubka centrifuge ultrafiltration xuubka tuubada waxaa lagu sifeeyay goynta miisaanka kelli (MWCO).
Miisaanka molecular solute ee u dhow MWCO waxa laga yaabaa in qayb ahaan la sii hayo. Si loo helo sifooyinka ugu wanaagsan ee xajinta,
fadlan isticmaal xuub filter MWCO oo leh miisaan kelli ah 1/3 ama ka yar solute.
Isbarbardhigga heerka soo kabashada alaabta
Isbarbardhigga heerarka soo kabashada borotiinka iyo tirada dhintay ee noocyada kala duwan ee tuubooyinka ultrafiltration
10KD Isbarbardhigga heerarka soo kabashada borotiinka iyo kuwa dhintay mugga noocyada kala duwan ee tuubooyinka ultrafiltration | |||
Summada | (%) Heerka soo kabashada | (μl) Mugga dhintay | |
BM | 1 ㎎/ml xoojinta borotiinka | 90 | 87 |
0.1㎎/ml xoojinta borotiinka | 83 | ||
Summada M | 1 ㎎/ml xoojinta borotiinka | 93 | 47 |
0.1㎎/ml xoojinta borotiinka | 88 | ||
Summada S | 1 ㎎/ml xoojinta borotiinka | 91 | 70 |
0.1㎎/ml xoojinta borotiinka | 85 | ||
Summada K | 1 ㎎/ml xoojinta borotiinka | 89 | 186 (U-fiirsashada borotiinka1㎎/ml) |
0.1㎎/ml xoojinta borotiinka | 77 | ||
Brand J | 1 ㎎/ml xoojinta borotiinka | 83 | |
0.1㎎/ml xoojinta borotiinka | 50 | ||
Xaaladaha tijaabada: borotiinka OVA (miisaanka kelli 45KD), rotor toosan 4000g | |||
Tilmaamaha xulashada tixraaca ee tuubooyinka ultrafiltration ee leh miisaanyo unugyo kala duwan
bilowga mugga | 0.5ml | 2 ml | 4ml | 15ml | |
Protein | |||||
(MW)miisaanka kelli | MWCO (Da) goynta miisaanka molecular | ||||
6K<MW20K | 3000 | 3000 | 3000 | 3000 | |
20K |MW 60K | 10000 | 10000 | 10000 | 10000 | |
60K |MW 100K | 30000 | 30000 | 30000 | 30000 | |
100K<MW200K | 50000 | 50000 | 50000 | 50000 | |
200K<MW | 100000 | 100000 | 100000 | 100000 | |
Asiidhyada nucleic-ka ah ee hal-xidhan iyo laba-geeska ah | |||||
dhererka | MWCO (Da) goynta miisaanka molecular | ||||
137-1159bp | 3000 | 3000 | 3000 | 3000 | |
nanoparticles | |||||
(DIA) cabbirka walxaha | MWCO (Da) goynta miisaanka molecular | ||||
1.5 DIA 3nm | 3000 | 3000 | 3000 | 3000 | |
3 DIA 5nm | 10000 | 10000 | 10000 | 10000 | |
5<DIA<7nm | 30000 | 30000 | 30000 | 30000 | |
7 DIA 10nm | 50000 | 50000 | 50000 | 50000 | |
10 nm DIIA | 100000 | 100000 | 100000 | 100000 |
Waqtiga boostada: Febraayo-18-2024