Ndeipi nzira yekuchenesa nayoprotein purification system? Izvo zvinodikanwa kuziva kurongeka kweDNA kutevedzana kweprotein yakacheneswa, kuona kuti ndeapi maseru kana matishu akanyanya kuwedzeredzwa mune inotarirwa gene, uye kugadzira gene primers kukwidziridza arf yeinonangwa chidimbu cheDNA. Uku ndiko kunonzi kutorwa kwezvimedu zvemajini.

Kuvaka kwekutaura vector: Mu prokaryotic kana eukaryotic expression vector ine hunhu hweiyo yakawanikwa gene kutaura pachayo, dambudziko guru redanho iri nderekuvaka plasmid uye gene rekufarira, uye yekutaura system. Iyo prokaryotic kutaura nguva ipfupi, mutengo wakaderera, uye huwandu hukuru hwekutaura chinhu chakakosha; jini haina kuratidzwa muE. coli, uye dambudziko rinoitika mukodon optimization. Mukufunga nezve basa riri nani uye kucheneswa kweprotein, vaongorori vakasarudza kutaura muPichi mbiriso. Kubudirira kutaura kwekodon optimization kwakakosha.

Ndeipi nzira yekuchenesa yeprotein yekuchenesa system:

1. Kunaya kwemvura.

2. Electrophoresis: Puroteni inopomerwa yakakwirira kana kuti yakaderera pane isoelectric point uye inogona kutamirwa kune electrode yakaipa kana kuti electrode yakanaka yemunda wemagetsi mumunda wemagetsi. Tsigira firimu electrophoresis, electrophoresis, nezvimwe.

3. Dialysis: Nzira inoshandisa mabhegi maviri edialysis kuti aparadzanise mamorekuru makuru kubva kumapuroteni uye madiki mamorekuru organic compounds.

4. Chromatography: Ion exchange chromatography inoshandisa mahara mapuroteni. Pasi peiyo pH chaiyo, machaji uye zvimiro zvemapuroteni zvakasiyana, uye anogona kupatsanurwa neion exchange chromatography. Mune anion exchange chromatography, mapuroteni ane simba rakaderera anotorwa kutanga. Masefa emolecular, anozivikanwawo sekuti gel filtration. Mapuroteni maduku anopinda mumapores uye anogara mavari kwenguva yakareba. Mapuroteni akakura haakwanise kupinda mumapores uye anoyerera achibuda zvakananga.

5. Ndeipi nzira yekuchenesa yeprotein purification system? Ultracentrifugation: Kucheneswa kweprotein kunogona kushandiswa kuona huremu hwema molecular uye inogona kushandiswa seprotein. Kuumbwa kwemapuroteni ane density yakasiyana inoparadzaniswa.