Oligonucleotide (Oligonucleotide), inowanzoreva mutsara wepolynucleotide fragment ye 2-10 nucleotide residues yakabatanidzwa ne phosphodiester bond, asi kana izwi iri richishandiswa, nhamba ye nucleotide residues Hapana mitemo yakasimba. Mumabhuku akawanda, polynucleotide mamorekuru ane makumi matatu kana anopfuura masara nucleotide anonziwo oligonucleotides. Oligonucleotides inogona kugadzirwa otomatiki nezviridzwa. Anogona kushandiswa seDNA synthesis primers, gene probes, nezvimwewo, uye ane huwandu hwakasiyana hwekushandisa mukutsvagisa kwemazuva ano molecular biology.

application

Oligonucleotides inowanzoshandiswa seprobes kuona chimiro cheDNA kana RNA, uye inoshandiswa mukuita senge gene chip, electrophoresis, uye fluorescence in situ hybridization.

Iyo DNA yakagadzirwa neoligonucleotide inogona kushandiswa muketani yepolymerization reaction, inogona kukwidziridza nekusimbisa zvinenge zvese zvidimbu zveDNA. Mukuita uku, iyo oligonucleotide inoshandiswa seprimer kusanganisa nechakanyorwa chinopindirana chimedu muDNA kuita kopi yeDNA. .

Regulatory oligonucleotides inoshandiswa kuvharidzira RNA zvimedu uye kudzivirira kuti zvisashandurwe kuva mapuroteni, ayo anogona kuita rimwe basa mukumisa basa rekenza maseru.