Solid Phase Extraction (SPE) muenzaniso wepretreatment tekinoroji yakagadziridzwa kubva pakati-1980s. Iyo inogadziriswa nekubatanidzwa kwe-liquid-solid extraction uye liquid chromatography. Inonyanya kushandiswa kuparadzanisa, kucheneswa uye kupfumisa masampuli. Chinangwa chikuru ndechekudzikisa sampuli matrix kupindira uye kugadzirisa kuona senitivity.

Zvichienderana nedzidziso yemvura-yakasimba chromatography, SPE tekinoroji inoshandisa yakasarudzika adsorption uye yakasarudzika elution kupfumisa, kuparadzanisa uye kuchenesa masampuli. Iyo inzira yekubvisa muviri inosanganisira mvura uye yakasimba zvikamu; inogona zvakare kuverengerwa nekuonekwa seyakapusa chromatographic maitiro.
Schematic dhayagiramu yechinhu chakasimba chekuwedzera chikamu
SPE ndiyo musimboti wekuparadzanisa wemvura chromatography uchishandisa yakasarudzika adsorption uye yakasarudzika elution. Iyo inonyanya kushandiswa nzira ndeyekupfuura iyo yemvura sampuli mhinduro kuburikidza neiyo adsorbent, chengetedza chinhu kuti chiongororwe, uyezve kushandisa chinonyungudutsa chesimba chakakodzera kubvisa tsvina, uye nekukasira kuburitsa chinhu kuti chiongororwe nehuwandu hushoma. solvent, kuitira kuti uwane chinangwa chekukurumidza kuparadzana, kuchenesa uye kuisa pfungwa. Zvinogonekawo kusarudza adsorb inopindira tsvina uye rega chinhu chakayerwa chibude kunze; kana kuti adsorb tsvina uye akapimwa fuma panguva imwe chete, uye ipapo kushandisa zvakakodzera solvent kusarudza elute chakayerwa chinhu.
The extractant of the solid-phase extraction method yakasimba, uye misimboti yayo yekushanda yakavakirwa pakuti zvikamu zvinoyerwa uye zvikamu zvinopindirana zvinopindirana mumucherechedzo wemvura zvine masimba akasiyana pane solid-phase extraction agent, zvekuti vakaparadzaniswa kubva kune mumwe. Solid chikamu chekubvisa mumiriri ndeye yakakosha filler ine C18 kana C8, nitrile, amino nemamwe mapoka.