Nucleic Acid Extraction System (Nucleic Acid Extraction System) chiridzwa chinoshandisa kuenzanisa nucleic acid yekubvisa reagents kuti ipedze otomatiki sampuli nucleic acid kutorwa. Inoshandiswa zvakanyanya munzvimbo dzekudzivirira zvirwere, kuongororwa kwechirwere chekiriniki, kuchengetedzeka kwekuwedzerwa ropa, chitupa chekuzivikanwa, kuongororwa hutachiona hwezvakatipoteredza, kuongororwa kwekuchengetedza chikafu, kupfuya mhuka uye mamorekuru biology kutsvagisa uye mamwe minda.
1. Nzira yekukweva, inozivikanwawo senzira yepipetting, ndeyekubvisa nucleic acid nekumisa magineti mabedhi uye kutamisa mvura. Kazhinji, kuchinjisa kunoitwa nekudzora robhoti ruoko kuburikidza neiyo inoshanda sisitimu. Nzira yekubvisa ndeyotevera:

1) Lysis: Wedzera lysis mhinduro kune sampuli, uye ziva kusanganiswa uye kuzere kuita kwemhinduro mhinduro kuburikidza ne mechanical kufamba uye kupisa, masero ari lysed, uye nucleic acid inosunungurwa.

2) Adsorption: Wedzera magineti mabhedhi kune sampuli lysate, sanganisa zvakakwana, uye shandisa magineti mabedhi kuti uve nehukama hwakasimba hwenyucleic acids pasi pemunyu wakanyanya uye yakaderera pH kuti adsorb nucleic acids. Pasi pechiito chekunze kwemagineti, magineti mabhesi anoparadzaniswa kubva kune mhinduro. , shandisa muromo kuti ubvise mvura uye uirase kutangi remarara, uye urase muromo.

3) Kugeza: Bvisa iyo yekunze magineti, tsiva netipi nyowani uye wowedzera washing buffer, sanganisa zvakanaka kubvisa tsvina, uye bvisa iyo mvura pasi pechiito chekunze kwemagineti.

4) Elution: Bvisa iyo yekunze magineti, tsiva neinotipi, wedzera elution buffer, sanganisa zvakanaka, wozoparadzanisa yakasungwa nucleic acid kubva kune magineti bead kuti uwane yakacheneswa nucleic acid.
2. Magnetic bar nzira

Iyo magnetic rod nzira inoona kupatsanurwa kwe nucleic acids nekugadzirisa mvura uye kuendesa magineti bead. Nheyo uye maitiro akafanana neaya enzira yekukweva, asi mutsauko inzira yekuparadzanisa pakati pemagineti bead uye mvura. Iyo magineti bar nzira ndeyekuparadzanisa magineti mabedhi kubva mumarara emvura kuburikidza neiyo adsorption yegineti tsvimbo kune magineti bead, woiisa mumvura inotevera kuona kubviswa kwenucleic acid.