SPME ine matatu akakoshakutorwamodes: Direct Ectraction SPME, Headspace SPME uye membrane-yakachengetedzwa SPME.

1) Kubudisa zvakananga

Mune yakananga yekubvisa nzira, iyo quartz fiber yakavharwa neiyokutorwastationary phase inopinzwa zvakananga mumuenzaniso matrix, uye izvo zvinonangwa zvinotamiswa zvakananga kubva kumuenzaniso matrix kuenda kune yekubvisa yakamira chikamu. Munguva yekushanda kwerabhoritari, nzira dzekukuchidzira dzinowanzo shandiswa kukurumidza kuparadzirwa kwezvikamu zvekuongorora kubva kumuenzaniso matrix kusvika kumucheto kwechikamu chekuburitsa chakamira. Kumasampuli egasi, kutenderera kwechisikigo kwegasi kunokwana kukurumidza kuenzana kwezvikamu zvekuongorora pakati pezvikamu zviviri. Asi kune sampuli dzemvura, kukurumidza kwekupararira kwezvikamu mumvura ndeye 3-4 mirairo yehukuru yakaderera pane iyo mumagasi, saka teknolojia yekubatanidza inoshanda inodiwa kuti uwane kukurumidza kupararira kwezvikamu mumuenzaniso. Iyo inonyanya kushandiswa kusanganisa nzira dzinosanganisira: kukurumidza kukurumidza kuyerera kwemuenzaniso, kuzunungusa iyo yekubvisa fiber musoro kana sampuli mudziyo, rotor inosimudzira uye ultrasound.

Kune rimwe divi, idzi nzira dzekusanganisa dzinomhanyisa chiyero chekupararira kwezvikamu zviri muhombe-vhoriyamu sampuli matrix, uye kune rimwe divi, kuderedza iyo inonzi "kurasikirwa zone" mhedzisiro inokonzerwa nedhizaini remvura firimu rinodzivirira sheath rakaumbwa pa. rusvingo rwekunze rwechikamu chekusimudza chakamira.

2) Headspace kutorwa

Mune iyo headspace yekubvisa modhi, iyo yekubvisa maitiro inogona kukamurwa kuita matanho maviri:
1. Chikamu chakaongororwa chinopararira uye chinopinda kubva pachikamu chemvura kusvika kune gasi chikamu;
2. Chikamu chakaongororwa chinotamiswa kubva pachikamu chegasi kuenda kuchikamu chekumisikidza.
Kugadziridzwa uku kunogona kudzivirira chikamu chekubvisa chakamira kubva pakusvibiswa neakakwira-mamorekuru zvinhu uye zvisiri-zvinoshanduka mune mamwe emuenzaniso matrices (akadai sekuvanzika kwevanhu kana weti). Mukuita kwekubvisa uku, kumhanya kwedanho rechipiri kunowanzo hukuru kupfuura kumhanya kwekupararira kwedanho 1, saka nhanho yekutanga inova nhanho yekudzora yekubvisa. Naizvozvo, zvinhu zvinoshanduka zvine kukurumidza kudhirowa mwero kupfuura semi-volatile components. Muchokwadi, kune zvikamu zvinoshanduka, pasi pemamiriro akafanana ekusanganisa mamiriro, nguva yekuenzanisa yekubvisa musoro ipfupi pane iyo yekubvisa zvakananga.

3) Membrane kudzivirira kubviswa

Chinangwa chikuru chekudzivirira membrane SPME kuchengetedzakutorwayakamira chikamu kubva mukukuvadzwa kana uchiongorora akasviba zvakanyanya samples. Kuenzaniswa neiyo headpace kudhirowa SPME, iyi nzira inonyanya kubatsira pakubvisa uye kupfumisa yezvakaoma-ku-kuvhuvhuta zvikamu. Mukuwedzera, iyo inodzivirira firimu yakagadzirwa nezvinhu zvakakosha inopa imwe dhigirii yekusarudza kwekuita kwekubvisa.