Kupatsanurwa uye kucheneswa kwemapuroteni kunoshandiswa zvakanyanya mukutsvagisa biochemistry uye kushandisa uye yakakosha hunyanzvi hwekuita. SCG Protein Purification System Kambani-Saipu Instrument yakanyora iyo isina kuchena kupatsanurwa uye yakanaka kupatsanura zvirimo zve.proteinkunatswa kumunhu wose. Sero rakajairwa eukaryotic rinogona kuve nezviuru zvemapuroteni akasiyana, mamwe akapfuma kwazvo uye mamwe ane mashoma makopi. Kuti udzidze imwe puroteni, zvinodikanwa kutanga kuchenesa mapuroteni kubva kune mamwe mapuroteni uye asiri mapuroteni mamorekuru.

Kuparadzana kwakaoma

Kana puroteni yakabviswa (dzimwe nguva yakasanganiswa ne nucleic acids, polysaccharides, nezvimwewo) inowanikwa, seti yenzira dzakakodzera inosarudzwa kuparadzanisa zvinodiwa.proteinkubva kune dzimwe tsvina. Kazhinji, danho iri rekuparadzanisa rinoshandisa nzira dzakadai semunyu kunze, isoelectric point accumulation uye organic solvent fractionation. Iyi nzira dzinoratidzwa nekureruka uye hukuru hwekugadzirisa simba, izvo zvinogona kubvisa tsvina yakawanda uye kuisa pfungwa paprotein solution. Mamwe mapuroteni anotorwa akakura muhuwandu uye haana kukodzera kusungirirwa nekuunganidza kana kuisa munyu kunze. Iwe unogona kusarudza ultrafiltration, gel filtration, kuomesa vacuum kuomesa kana dzimwe nzira dzekuisa.

Kuparadzana kwakanaka

Mushure mekugovewa kwakashata kwemuenzaniso, vhoriyamu kazhinji idiki, uye yakawanda yetsvina yakabviswa. Kuti uwedzere kucheneswa, nzira dzechromatography dzinowanzobatanidza kusefa kwegel, ion exchange chromatography, adsorption chromatography, uye affinity chromatography. Kana zvichidikanwa, unogonawo kusarudza electrophoresis, kusanganisira zone electrophoresis, isoelectric point set, nezvimwewo seyokupedzisira yekuchenesa nzira. Nzira inoshandiswa pakupatsanurwa kwechikamu chechikamu kazhinji idiki mukuronga, asi nekugadziriswa kwepamusoro.

Crystallization ndiyo yekupedzisira maitiro ekuparadzanisa mapuroteni uye kucheneswa. Kunyangwe iyo crystallization process isingaite kuti puroteni inofanirwa kuve yakafanana, zvinongoitika kana imwe puroteni ine mukana mune mhinduro yekuumba kristaro. Iyo crystallization process pachayo inoperekedzwa neimwe dhigirii yekucheneswa, uye recrystallization inogona kubvisa shoma shoma yeprotein yakasvibiswa. Kubva denaturedproteinhaina kumbobvira yawanikwa panguva yekristallisation process, protein crystallization haisi chiratidzo chekuchena chete, asiwo nhungamiro ine simba yekuona kuti chigadzirwa chiri mumamiriro ayo echisikigo.