Yedu Inopisa-Kutengesa Zvigadzirwa Ultrafiltration Centrifugal Tube (Centrifugal Sefa)Series Zvigadzirwa

------" Ultrafiltration & Microfiltration " Multipurpose, uchiziva kutsiva mudzimba!

BM Hupenyu Sayenzi,Ultrafiltration Centrifugal Tube (Centrifugal Sefa)Series Zvigadzirwa

Iyo ine yemukati sefa chubhu (ine membrane) + yekunze centrifuge chubhu, inogona kukurumidza uye zvinobudirira kuchenesa uye kuisa pfungwa kusvika ku15ml yemasampuli ehupenyu. Iyo yakasarudzika vertical dhizaini uye yakakura filtration nzvimbo inogona kupa nekukurumidza sampuli yekugadzirisa simba uye yakakwira sampuli yekudzoreredza mwero (kazhinji ≥90% dilute yepakutanga mhinduro), uchichengetedza yakapfava kusefa uye yekumisikidza nharaunda kuchengetedza mapuroteni chiitiko uye Conformation.Iyo yakasarudzika vertical dhizaini inogona polarize iyo solute uye inoderedza zvakanyanya kusvibiswa kwesefa membrane inokonzerwa neinotevera centrifugation. Nekugadzirisa iyo yemuviri sefa yekumisa poindi musefa mudziyo, inogona kudzivirira iyo centrifugal sefa kubva pakuomesa sampuli kana kukonzera kurasikirwa kwemuenzaniso nekuda kwekutenderera kwakanyanya. . Yakasiyana-siyana ye Ultrafiltration molecular weight cutoff (MWCO) centrifugal mafirita anoshandisa yakakwirira-kuchena, yakaderera-adsorption PES uye RCE membranes, ine yakaderera zvakanyanya mapuroteni uye nucleic acid inosunga mazinga.

BM Hupenyu Sayenzi, yakachena kamuri kugadzirwa mune zvese zvinongedzo zveUltrafiltration Centrifugal Tube (Centrifugal Sefa), kushanda kwemutsara wegungano, kutonga kwakazara kweERP, ultra-pure products, hapana DNase / RNase, hapana PCR inhibitors, hapana kupisa. BM Hupenyu Sayenzi, ese masaizi eUltrafiltration Centrifugal Tube (Centrifugal Sefa)zvigadzirwa zvakatevedzana zvakagadzirirwa, nhevedzano yezvigadzirwa yakagadzikana mumabhechi ane misiyano mishoma pakati pemabhechi uye akakwira mumhando. Inogona kutsiviwa nezvigadzirwa zvemumba uye kutumirwa kuJapan, South Korea, Europe, uye United States. Inoshandiswa zvakanyanya mune yakajairwa sampuli yekuparadzanisa uye kusefa kuyedza.

Application

1 UltrafiltrationBuffer kutsiva, kucheneswa, kuisa pfungwa, uye kubvisa munyu kwemaantigens, masoja ekudzivirira chirwere, ma enzymes, uye mamwe mapuroteni, pamwe nemasampuli ehupenyu akadai senyucleic acids uye ma microorganisms, desalination ye chromatographic components, kupatsanurwa kwemamorekuru ebhayoloji kubva kumatishu tsika kana sero lysis mhinduro, kubvisa. zvemaprimers, zvinobatanidza, kana mamorekuru mamaki kubva kune reaction musanganiswa, hutachiona hwehutachiona kana kupatsanurwa, uye kupatsanurwa kwakashata kwebiological molecule musanganiswa.

2 Microfiltrationinoparadzanisa nucleic acids uye mapuroteni mumajeri, sampuli dzinocheneswa uye kujekeswa, uye zvidimbu zvakagadziriswa kana mabhesi anounganidzwa uye anogezwa.

Feature yeChigadzirwa

High concentration ratio, iyo inogona kusvika nyore 80-100 times concentration ratio;

Sample yekumhanyisa kumhanya inokurumidza, uye iyo general concentration nguva ndeye 10-60 maminetsi. Iyo zvakare ine anti-kuomesa yekiyi dhizaini kudzivirira kukuvadzwa kune sampuli nekuda kwekunyanyisa centrifugation;

★ Muenzaniso wekudzoreredza mwero wakakwira uye unogona kusvika padanho rekudzoreredza rinopfuura 90%;

★ Yakaderera adsorption, PES/RC membrane uye yakatsetseka yemukati madziro dhizaini ine yakanyanya kuderera protein adsorption uye biomolecule inosunga mwero;

4ml/15ml, 15ml/50ml

★ Kunyatsotsanangurwa kwakazara, ultrafiltration centrifuge tubes ane mavhoriyamu maviri e4ml/15ml uye 15ml/50ml, ane hukuru hwakasiyana hwepore, anowanikwa mu3KD, 5KD, 10KD, 30KD, 50KD uye 100KD kuti isangane neyakasiyana molecular weight cutoff;

★ Kushanda kuri nyore, iyo yese yekuparadzanisa uye kusefa maitiro inogona kupedzwa neMultifunctional Centrifuge, nyore kushanda;

★ Chigadzirwa chacho chakachena, zvose zvinongedzo zvinogadzirwa muimba yakachena, mutsara wekushanda, hutungamiri hwakazara ERP, chigadzirwa chacho chinonzi ultra-pure, hapana DNase / RNase, hapana PCR inhibitor, hapana chitubu chekupisa;

★ Tsanangudzo yepamusoro, isina kumashure uye auto-fluorescence, uye haikanganisi mibairo yekuona;

★ Kuita kwakavimbika, kuiswa chisimbiso kwakanaka, uye hakuite nemasampuli ekuedza, mhedzisiro yekuyedza yakavimbika, uye inogona kumira tembiricha yakakwira ye100 ℃!

★ Iine huwandu hwakawanda hwekushandisa. Kune akasiyana maapplication, maviri marudzi eUltrafiltration Centrifugal Tube (Centrifugal Sefa)zvigadzirwa zvakatevedzana, 4ml/15ml uye 15ml/50ml zvinounzwa kuti zvisangane nezvinodiwa nevatengi zvakasiyana, kubvuma kugadzirisa uye kusimudzira kushanda!

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avsdv (4)
avsdv (5)
avsdv (6)

Order Information

Katsi.No Zita Tsanangudzo Tsanangudzo Pcs/pk
UCT015RCE004003 Ultrafiltration Centrifugal Tube 4/15ml, 3kD , RCE Membranes 15 pcs / bhegi
UCT015RCE004010 Ultrafiltration Centrifugal Tube 4/15ml, 10kD RCE Membranes 15 pcs / bhegi
UCT015RCE004030 Ultrafiltration Centrifugal Tube 4/15ml, 30kD RCE Membranes 15 pcs / bhegi
UCT015RCE004100 Ultrafiltration Centrifugal Tube 4/15ml, 100kD RCE Membranes 15 pcs / bhegi
UCT050RCE015003 Ultrafiltration Centrifugal Tube 15/50ml, 3kD RCE Membranes 24 pcs / bhegi
UCT050RCE015010 Ultrafiltration Centrifugal Tube 15/50ml, 10kD RCE Membranes 24 pcs / bhegi
UCT050RCE015030 Ultrafiltration Centrifugal Tube 15/50ml, 30kD RCE Membranes 24 pcs / bhegi
UCT050RCE015100 Ultrafiltration Centrifugal Tube 15/50ml, 100kD RCE Membranes 24 pcs / bhegi
UCT050PES015005 Ultrafiltration Centrifugal Tube 15/50ml, 5kD PES Membranes 12 pcs / bhegi
UCT050PES015010 Ultrafiltration Centrifugal Tube 15/50ml, 10kD PES Membranes 12 pcs / bhegi
UCT050PES015030 Ultrafiltration Centrifugal Tube 15/50ml, 30kD PES Membranes 12 pcs / bhegi
UCT050PES015050 Ultrafiltration Centrifugal Tube 15/50ml, 50kD PES Membranes 12 pcs / bhegi
UCT050PES015100 Ultrafiltration Centrifugal Tube 15/50ml, 100kD PES Membranes 12 pcs / bhegi
CF050PES015C010 Sefa yeCentrifugal 15/50ml, 0.1um 0.1um, PES Membranes 12 pcs / bhegi
CF050PES015C020 Sefa yeCentrifugal 15/50ml, 0.2um 0.2um, PES Membranes 12 pcs / bhegi
CF050PES015C045 Sefa yeCentrifugal 15/50ml, 0.45um 0.45um, PES Membranes 12 pcs / bhegi
UCT** Ultrafiltration Centrifugal Tube Customizing Customizing ** pcs/bhegi
CF** Sefa yeCentrifugal Customizing Customizing ** pcs/bhegi

BM Hupenyu Sayenzi, Ultrafiltration Centrifugal Tube (Centrifugal Filter) akatevedzana zvigadzirwa paramita uye yekuedza data.

Ultrafiltration Centrifugal Tube (Centrifugal Filter) akatevedzana chigadzirwa kuita uye kuyedza data

avsdv (8)
avsdv (9)

Ultrafiltration Centrifugal yemasampuli:

5 KD 0.25mg/ml Cyt C;

10 KD1mg/ml OVA;

30/50 KD 1mg/ml BSA;

100 KD 1mg/ml IgG.

Mamiriro ekuedza: yakachinjika rotor, centrifugal simba 4000 × g, 8 ℃, kutanga vhoriyamu 15ml.

Ultrafiltration Centrifugal yemasampuli:

5 KD 0.25mg/ml Cyt C;

10 KD 1mg/ml OVA;

30/50 KD 1mg/ml BSA;

100 KD 1mg/ml IgG.

Mamiriro ekuedza: yakagadziriswa angle rotor, centrifugal simba 5000 × g, 8 ℃, kutanga vhoriyamu 12ml.

Yakajairika yekurambwa mwero yeprotein molecular uremu zviyero (4000g x 15ml kutanga vhoriyamu yekuzunguza rotor kana 5000g x 12ml yekutanga vhoriyamu yeyakagadziriswa angle rotor)

molecular weight standards/concentration molecular uremu NMWL Ultrafiltration chubhu kurambwa % Seta bhaketi Rejection rates % fixed angle centrifugation nguva
B-12 vitamini 1350 3K <25 <25 60
(0.25mg/ml) Cytochrome C 12400   >95 >95 60
(1mg/ml) α-Chymotrypsinogen 25000   >95 >95 60
B-12 (0.2mg/ml) vhitamini 1350 10K <5 <5 30
0.25mg/ml) Cytochrome C 12400   >95 >95 30
(1mg/ml) α-Chymotrypsinogen 25000   >95 >95 30
vhitamini B-12 (0.2mg/ml) 1350 30K <5 <5 20
Cytochrome C 0.25mg/ml) 12400   <10 <10 20
Ovalbumin (1mg/ml) 45000   >95 >95 20
BSA (1mg/ml) 67000   >95 >95 20
Cytochrome C (0.25mg/ml) 12400 50K <5 <5 10
Ovalbumin (1mg/ml) 45000   65 55 10
BSA (1mg/ml) 67000   >90 >90 10
Ovalbumin (1mg/ml 45000 100K <25 <15 20
IgG (1mg/ml) 156000   >90 >90 20
Thyroglobulin (0.5mg/ml) 677000   >90 >90 20

Recovery rate

Kukwanisa kuchengetedza kwe15ml ultrafiltration centrifuge tube membrane inotsanangurwa ne molecular weight cutoff (MWCO).

Solute molecular weights padyo neMWCO inogona kungochengetwa zvishoma. Kuti uwane akanakisa ekuchengetedza maitiro,

ndapota shandisa MWCO sefa membrane ine molecular uremu hwe 1/3 kana pasi pe solute.

Chigadzirwa chekudzorera chiyero kuenzanisa

avsdv (10)

Kuenzanisa kweprotein kudzoreredza mazinga uye akafa mavhoriyamu emhando dzakasiyana dze ultrafiltration machubhu

10KD Kuenzanisa kweprotein kudzoreredza mitengo uye akafa

mavhoriyamu emhando dzakasiyana dze ultrafiltration chubhu

Brand (%) Recovery rate (μl) Vhoriyamu yakafa
BM 1㎎/ml Kuiswa kweprotein 90 87 (Protein concentration1㎎/ml)
0.1㎎/ml Protein concentration 83  
Brand M 1㎎/ml Kuiswa kweprotein 93 47 (Protein concentration1㎎/ml)
  0.1㎎/ml Protein concentration 88  
Brand S 1㎎/ml Kuiswa kweprotein 91 70 (Protein concentration1㎎/ml)
  0.1㎎/ml Protein concentration 85  
Brand K 1㎎/ml Kuiswa kweprotein 89 186 (Protein concentration1㎎/ml)
  0.1㎎/ml Protein concentration 77  
Brand J 1㎎/ml Kuiswa kweprotein 83  
  0.1㎎/ml Protein concentration 50  
  Mamiriro ekuedza: OVA protein (molecular uremu 45KD), yakachinjika rotor 4000g
 

Reference kusarudzwa kutsanangurwa kweiyo ultrafiltration machubhu ane akasiyana ma molecular uremu

  kutanga volume <0.5ml <2ml <4ml <15ml
Mapuroteni
(MW) molecular uremu   MWCO (Da) molecular uremu cutoff      
  6K<MW<20K 3000 3000 3000 3000
  20K<MW<60K 10000 10000 10000 10000
  60K<MW<100K 30000 30000 30000 30000
  100K<MW<200K 50000 50000 50000 50000
  200K<MW 100000 100000 100000 100000
Single-stranded uye kaviri-stranded nucleic acids
urefu   MWCO (Da) molecular uremu cutoff      
  137-1159bp 3000 3000 3000 3000
nanoparticles
(DIA) size   MWCO (Da) molecular uremu cutoff      
  1.5<DIA<3nm 3000 3000 3000 3000
  3<DIA<5nm 10000 10000 10000 10000
  5<DIA<7nm 30000 30000 30000 30000
  7<DIA<10nm 50000 50000 50000 50000
  10 nm<DIA 100000 100000 100000 100000

Nguva yekutumira: Feb-18-2024