Solid phase extraction (SPE) inzira yekubvisa yemuviri inosanganisira mvura uye yakasimba zvikamu. Mukuita kwekubvisa, simba rekushambadza reiyo yakasimba kune analyte yakakura kupfuura sampuli yaamai doro. Kana sampuli ichipfuura nepakatiSPEcolumn, analyte inoshambadzirwa panzvimbo yakasimba, uye zvimwe zvikamu zvinopfuura nemukoramu nemuenzaniso wedoro raamai. Pakupedzisira, analyte inobviswa neakakodzera solvent Eluted. SPE ine zvakawanda zvekushandisa, zvakadai sekuongororwa kwemvura yehupenyu inosanganisira ropa, weti, serum, plasma uye cytoplasm; kuongororwa kwekugadziriswa kwemukaka, waini, zvinwiwa nemichero yemichero; kuongororwa nekutariswa kwezviwanikwa zvemvura; michero, miriwo, zviyo, uye siyana chirimwa tishu Mhuka tishu; mishonga yakasimba yakaita semapiritsi. Ongororo yemishonga yezvipembenene uye herbicide masara mumichero, miriwo uye chikafu, kuongororwa kwemishonga inorwisa mabhakitiriya uye mishonga yekiriniki, nezvimwe.

(1) Nyatso tora iyo yakasimba chikamu chekubvisa mudziyo woiisa zvinyoro nyoro pabhenji rebasa.

(2) Nyatsobvisa chivharo chepamusoro cheSPEmudziyo (bata zvinyoro-nyoro kuti usakuvadza chubhu diki), isa iyo yakajairwa test chubhu mugomba rekuparadzanisa mukamuri yekutsvaira, wobva wavhara chivharo chepamusoro chakaoma, uye chengetedza kuti chivharo chinotungamirirwa pasi. Iyo inoyerera chubhu uye test chubhu inoenderana imwe neimwe, uye sikweya yekuisa chisimbiso mhete yevhavha ndiro ine yakanaka yekuisa chisimbiso nekamuri yekutsvaira. Kana zvisiri nyore kuvhara, inogona kusimbiswa nerubber band kuti iwedzere kusimba.

(3) Kana iwe wakatenga yakazvimiririra kugadzirisa, iwe unofanira kutanga waisa valve yekugadzirisa mugomba rekubvisa revhavha;

(4) Kana usingade kuita 12 kana 24 samples panguva imwe, sunga tsono chubhu tight valve mugomba risingashandiswe rekubvisa;

(5) Kana vharuvhu yekudzora yakazvimiririra yakatengwa, shandura bharuvhu yekudzora yegomba risingashandiswe rekubvisa kune yakachinjika yekuisa chisimbiso;

(6) Isai iyo yakasimba chikamu chekubvisa cartridge mugomba rekudhonza kana gomba revharuvha yevhavha yepamusoro (shandura bhavhu revharuvhu rinogadzirisa kune yakavhurika nyika yakatwasuka); batanidza mudziyo wekubvisa uye pombi yevacuum nehose, uye simbisa iyo inodzvinyirira inodzora vharafu;

(7) Majekiseni masampuli kana reagents kuti abudiswe mumutsara wekubvisa, uye tanga pombi yevacuum, ipapo sampuli mumutsara wekuchera ichayerera kuburikidza nekoramu yekuchera kusvika kune test tube iri pasi pechiito chekumanikidza kusina kunaka. Panguva ino, kuyerera kwemvura kunogona kugadziriswa uye kudzorwa nekugadzirisa kudzvinyirira kuderedza valve.

8

(9) Kana iwe usingade kushandisa test chubhu kubatanidza mvura, unogona kutora test tube rack, woiisa mumudziyo wehukuru hwakakodzera, uye woiburitsa mushure mekutanga kudhirowa.

(10) Isa yakachena test chubhu mumudziyo, vhara chivharo, isa SPE katuriji, wedzera chinodiwa chekubvisa chinonyungudutsa pachubhu yetsono, tanga vacuum pombi, dzima simba mushure mekunge mvura yadonha, uye bvisa iyo. test tube yekushandisa. Kudhirowa uye sampuli kugadzirira kwapera.

(11) Isa chubhu yekuyedza mumudziyo wekuomesa nitrogen wochenesa nekunangana nenitrogen, uye kugadzirira kwapera.

(12) Rasa chinonyungudutsa muchubhu yebvunzo, wogeza chubhu yebvunzo kuti uishandise zvakare.

(13) Kuti uchengetedze mutengo wekushandisaSPEcolumn, mushure mekushandisa kwega kwega, iyo SPE koramu inofanirwa kugezwa ne-eluent kuti ive nechokwadi chezvimiro zvekurongedza kwayo.