Iyo yekuenzanisira bhodhoro mudziyo wekuongorora chiridzwa chechinhu chiongororwe, uye kuchena kwayo kunobata zvakananga mhinduro yekuongorora. Ichi chinyorwa chinopfupikisa nzira dzakasiyana-siyana dzekuchenesa chromatographic sampuli bhodhoro, uye ine chinangwa chekupa chirevo chine musoro kune wese munhu. Idzi nzira dzakasimbiswa neshamwari nevakatangira. Ivo vane yakanaka yekugezesa mhedzisiro pamafuta-anonyungudika masara uye organic reagent masara muchromatography muenzaniso bhodhoro. Utsanana hunosangana nezvinodiwa, matanho ekuchenesa ari nyore, uye nguva yekuchenesa inoderedzwa, uye nzira yekuchenesa inonyanya kushamwaridzana .

Ndapota ita sarudzo yako pachako zvichienderana nemamiriro ako erabhoritari!

Parizvino, nekuwedzera kufarira kwemhando yechikafu uye kuchengetedzeka kubva kumatanho ese ehupenyu, tekinoroji yekuongorora chromatographic iri kuwedzera kushandiswa mumhando yechikafu uye kuongororwa kwekuchengetedza, kunyanya mundima yekuongorora chigadzirwa chekurima, tekinoroji yekuongorora chromatographic yakashandiswa zvakanyanya. Munyika yangu, nhamba huru yezvigadzirwa zvekurima (zvimwe zvigadzirwa zvemakemikari, organic acids, nezvimwewo) zvinoda kuongororwa ne liquid chromatography uye gasi chromatography gore negore. Nekuda kwehuwandu hukuru hwemasamples, kune huwandu hukuru hwemabhodhoro emuenzaniso anoda kucheneswa panguva yekuonekwa, izvo zvisingangotambisi nguva uye kuderedza kushanda zvakanaka, asi dzimwe nguva zvinokonzeresa kutsauka kwemhedzisiro yekuedza nekuda kwekuchena kweiyo. mabhodhoro emuenzaniso akacheneswa.

Thechromatographic sample bhodhoroinonyanya kugadzirwa negirazi, kashoma plastiki. Mabhodhoro emuenzaniso anoraswa anodhura, anoparadza, uye anokonzera kusvibiswa kwezvakatipoteredza. Marabhoritari mazhinji anochenesa mabhodhoro emuenzaniso uye oashandisazve. Parizvino, nzira dzinowanzo shandiswa mumarabhoritari kuchenesa bhodhoro remuenzaniso dzinonyanya kuwedzera hupfu hwekugezesa, detergent, organic solvent, uye acid-base lotion, wozokwesha nechubhu diki yekuyedza. Iyi yakajairika nzira yekukwesha ine zvakawanda zvinokanganisa. Inoshandisa yakawanda ye detergent nemvura, inotora nguva yakareba yekugeza, uye inowanzosiya makona akafa. Kana iri bhodhoro remuenzaniso wepurasitiki, zviri nyore kusiya mavara ebhurashi pamadziro ebhodhoro remukati, izvo zvinotora huwandu hwakawanda hwevanhu. Kune glassware iyo yakasvibiswa zvakanyanya ne lipid uye mapuroteni masara, alkaline lysis solution inoshandiswa pakuchenesa, uye zvibereko zvakanaka zvinowanikwa.

Paunenge uchiongorora samples, kucheneswa kwebhodhoro rejekiseni kwakakosha. Maererano negirazi rekugeza nzira, nzira yekuchenesa inosarudzwa maererano nehuwandu hwekusvibiswa, uye hapana yakagadziriswa mode. Nzira pfupiso:

1. Dururira mhinduro yekuedza mubhodhoro rakaoma

2. Inyura zvose mu 95% doro, ugeze kaviri ne ultrasonic uye uidururire kunze, nokuti doro rinopinda nyore nyore muvharo re 1.5mL uye rinogona kusanganiswa nemafuta akawanda emakemikari kuti awane kushanda kwekuchenesa.

3. Dururira mumvura yakachena, uye ultrasonically geza kaviri.

4. Dururira lotion mubhodhoro rakaoma uye gadzira pa 110 degrees Celsius kwe 1 ~ 2 maawa. Usambofa wakabika pane kupisa kwakanyanya.

5. Kutonhorera uye chengetedza.