Iyo general maitiro eiyo solid phase kubviswa ndeiyi inotevera:

1. Kushandisa adsorbent: Shamba zvakasimba chikamu chekubvisa cartridge neine solvent yakakodzera usati watora sampuli kuti uchengetedze adsorbent yakanyorova, iyo inogona kushambadzira zvinonangwa zvinosanganiswa kana kupindira kwemakemikari. Mhando dzakasiyana dzeiyo yakasimba chikamu chekuwedzera cartridge activation inoshandisa zvakasiyana zvinonyungudutsa:

(1) Neak polar or non-polar adsorbents inoshandiswa mu reversed-phase solid-phase extraction inowanzosukurudzwa nemvura-soluble organic solvent, yakadai semethanol, uye yozosukwa nemvura kana kuti buffer solution. Izvo zvakare zvinokwanisika kugezesa neakasimba solvent (yakadai sehexane) usati wageza ne methanol kubvisa tsvina yakashongedzwa pane adsorbent uye kupindira kwavo kune chinangwa chekomboni.

(2) Iyo polar adsorbent inoshandiswa mune yakajairwa-phase solid-phase extraction inowanzo bviswa ne organic solvent (sample matrix) uko kunowanikwa komboni inotangwa.

(3) Iyo adsorbent inoshandiswa muion-exchange solid phase extraction inogona kushambidzwa nesample solvent kana ichishandiswa sampuli mune zvisiri-polar organic solvents; kana ichishandiswa sampuli mu polar solvents, inogona kushambidzwa nemvura-soluble organic solvents Mushure mekugeza, sukurudza nemushonga wemvura wakakodzera pH kukosha uye uine mamwe organic solvents nemunyu.

Kuti uchengetedze sorbent mu SPE cartridge yakanyorova mushure mekushanda uye isati yasvika sampuli yekuwedzera, inenge 1 ml yemushonga wekugadzirisa unofanirwa kuchengetwa pane sorbent mushure mekushanda.

2. Sample loading: Dururirai mvura kana kuti yakanyungudutswa yakasimba sampu mukati meiyo activated solid phase extraction cartridge, wobva washandisa vacuum, pressure kana centrifugation kuita sample ipinde muadsorbent.

3. Kushamba uye kusvibiswa: Mushure mokunge sampu yapinda mu adsorbent uye chinangwa chinoshandiswa chinoshandiswa, iyo isina simba yakachengetwa inopindira inogona kushambidzwa nemhepo isina simba, uye ipapo chidimbu chinotarirwa chinogona kubviswa nechisimba chakasimba uye chinounganidzwa. . Rinse uye Elution Sezvakatsanangurwa kare, iyo eluent kana eluent inogona kupfuura kuburikidza adsorbent nenzira vacuum, pressure kana centrifugation.

Kana iyo adsorbent yakasarudzwa kuti ive isina kusimba kana kuti isina adsorption kune iyo inotarirwa komputa uye yakasimba adsorption kune inopindirana inopindirana, iyo yakanangwa komputa inogonawo kucheneswa uye kuunganidzwa kutanga, nepo chidimbu chinopindira chinochengetwa (adsorption). ) pane adsorbent, maviri akaparadzaniswa. Muzviitiko zvakawanda, chinangwa chemukomboni chinochengetwa pane adsorbent, uye pakupedzisira yakasvibiswa neine solvent yakasimba, iyo inonyanya kubatsira pakucheneswa kwemuenzaniso.