Blot Analysis muBiopharmaceutical, Medical uye Mamwe Mamunda

Chirongwa che "14th-Makore mashanu" chirongwa cheBioeconomy Development Plan chinokurudzira kuti bioeconomy inofanirwa kufambiswa nebudiriro uye kufambira mberi kwesainzi yehupenyu nebiotechnology, zvichibva pakuchengetedzwa, kusimudzira uye kushandiswa kwezviwanikwa zvebiology, uye zvichibva mukubatanidzwa kwakadzama uye kwakadzama. mushonga, hutano, kurima, masango, uye simba. , kuchengetedza kwezvakatipoteredza, zvinhu uye mamwe maindasitiri; zviri pachena kuti kusimukira kwebio-economy igwara rakakosha kutevedzera kukurumidza kushanduka kwepasi rose biotechnology uye kuwana hunyanzvi hwekuzvimirira hwepamusoro hwesainzi uye tekinoroji. Icho chiyero chakakosha kurima nekuwedzera iyo bio-indasitiri uye kusimudzira hupfumi hwemhando yepamusoro. Kuzadzikisa kukurumidza kukura kwehupenyu nezvinodiwa zvehutano uye kugutsa chishuwo chevanhu chehupenyu huri nani vimbiso yakakosha yekusimbisa kuchengetedzwa kwenjodzi yenyika nekudzora nekusimudzira kuvandudzwa kwehurongwa hwekutonga kwenyika uye kugona kwekutonga.

Mukupindura kudanwa kwenyika, BM yakazvipira kukunda tekinoroji yekugadzira mafirimu epamusoro uye zvishoma nezvishoma nekuona kutsiva kwekunze kwezvinhu zvinodhura zvakanyanya mumunda wesainzi yehupenyu. Muna Chivabvu 2023, kugadzirwa kwakawanda kwe immunochromatography NCmembranes yakawanikwa zvakabudirira uye yakashandiswa kune akasiyana siyana anokurumidza kuona reagents. Parizvino, firimu reNC rave richishandiswa mudzimba in vitro diagnostics, kuchengetedzwa kwechikafu, kukurumidza kuyedza zvinodhaka uye mamwe minda, uye yakawana reverse kunze kwenyika uye inokwikwidza hofori dzepasirese pamusika! Mushure mekupedza hurukuro yemusika wemufirimu weNC, mushure memwedzi yakati wandei yekutsvagisa tekinoroji nechikwata chedu chehunyanzvi, mukupindura kune zvinodikanwa zvevashandisi mundima yesainzi yehupenyu hwepasirese kudzikisa mutengo wezvakanyanya kukosha-kukosha kwekushandisa, isu takabudirira kutanga kudzima.membranes, iyo yakakodzera biopharmaceuticals, mushonga uye mamwe minda. Kuongorora kweWestern blot (Western Blotting, WB)

Nhanganyaya yezvimiro zveBM Blotting Membranes, : Saizi yePore uye inoshanda mapuroteni erudzi 0.1μm akakodzera mapuroteni ane huremu hwemamorekuru asingasviki 7kDa 0.22μm akakodzera mapuroteni ane huremu hwemamorekuru asingasviki 20kDa 0.45μm akakodzera mapuroteni ane huremu hwemamorekuru kupfuura 20kDa Main protein binding principles Static magetsi uye hydrophobicity Mamiriro ekufambisa anoshanda uye nzira dzekuona Chemiluminescence Fluorescence yekuona radiolabeled probe yakananga kudhaya Enzyme-yakabatana antibody Advantage:

1.Low background, high sensitivity

2.No kudiwa doro reagent pre-wetting

3.Unique pamusoro pemaitiro uye zvivakwa zvinogadzira yakanakisa chiratidzo-ku-ruzha chiyero Iyo nyaya inotorwa kubva kune yakasikwa fibers, inoshamwaridzana nemhoteredzo, uye inogona kuchengetedza yakasungwa protein inoshanda kwenguva yakareba.

Nhanganyaya yeWB yekuongorora tekinoroji WB yekuongorora tekinoroji tekinoroji inoshandiswa zvakanyanya mundima dzemolecular biology, biochemistry, immunology uye mamwe minda. Iyi tekinoroji inoshandisa chaiyo inosunga masoja ekudzivirira chirwere kumapuroteni chaiwo ari munyama kana maseru sampuli kuti awane kuzivikanwa kweprotein uye ongororo yekutaura zvichienderana nechinzvimbo uye kusimba kwebhendi reruvara, ndiko kuti, hunhu uye semi-quantitative ongororo. Yakatanga kutaurwa naHarry Towbin weFriedrich Miescher Institute muSwitzerland muna 1979. Pave nemakore anopfuura makumi mana apfuura uye yave nzira yemhando yepamusoro uye inoshanda yeprotein yekutsvaga.